Studies on the effect of diesel exhaust particles on autoimmune disease and its mechanisms
| Project/Area Number |
09672237
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Saga Medical School |
|---|
Principal Investigator |
YOSHINO Shin Saga Medical School Microbiology Assistant Professor, 医学部, 講師 (00260729)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | diesel exhaust particles / autoimmune disease / collagen arthritis / rheumatoid arthritis / cytokine |
|---|
| Research Abstract |
The present study was undertaken to investigate the effect of diesel exhaust particles (DEP) on collagen-Induced arthritis (CiA) in mice which Is an experimental model of autolmmune disease. CIA was induced by subcutaneous injection of type II collagen (CII) emulsified with complete Freund's adjuvant Into the base of the tall (day 0) followed by a booster injection on day 21. Varying doses of DEP were intranasally administered every 2 days from day 0 to day 20. The results showed that administration of DEP enhanced both the Incidence and the severity of CIA.The enhancement of the disease was associated with pronounced production of anti-CII IgG and IgG2a antibodies. Treatment with DEP also augmented proliferative responses of spleen cells to CII.There was marked secretion of IFN-gamma, IL-2, and IL-4 from the lymphoid cells in DEP-treated mice. Administration of DEP after onset of CIA was also effective in enhancing the severity of the disease as well as production of anti-CII IgG and IgG2a antibodies and secretion of IFN-gamma , IL-2, and IL-4. These results suggest that exposure to DEP may influence autoimmune disease.
|
Report
(3 results)
Research Products
(24 results)
