| Project/Area Number |
09672238
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
SHOJI Shozo Kumamoto Univ., Pharmaceutical Sciences, Professor, 薬学部, 教授 (60040317)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Ichiro Teikyo Univ., School of Medicine, Instructor, 医学部, 講師 (40091045)
HORIUCHI Seiko Kumamoto Univ., School of Medicine, Professor, 医学部, 教授 (10117377)
MISUMI Shogo Kumamoto Univ., Pharmaceutical Sciences, Researcher, 薬学部, 助手 (40264311)
FURUISHI Kazuchika Kumamoto Univ., Pharmaceutical Sciences, Researcher, 薬学部, 助手 (40238663)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | HIV-1 / N-Myristoylation / Bystander effect / HiIV-1 |
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| Research Abstract |
The role of the N-myristoylation of the human immunodeficiency virus type 1 (HIV- 1) gag protein in ACH-2 cells was studied. The infectivity of HLV-1 from the cells stimulated with phorbol 12-myristate 13-acetate (PMA) was suppressed by pretreatment with N-myristoyl glycinal diethylacetal (N-Myr-GOA), a potent N-myristoylation inhibitor, and the blockage of myristoylation resulted in accumulation of immature gag precursors. The viral particles which budded from the non-N-Myr-GOA-treated ACH-2 cells stimulated with PMA exhibited a typical viral phenotype, whereas those which budded from the N-Myr-GOA-treated ACH-2 cells stimulated with PMA were twisted, as observed electron microscopically. In electron microscopic analyses with gold-labeled monoclonal antibodies to gag and env, gag and env were detected adjacent to each other in the PMA-stimulated ACH-2, but no env was detected in the cells treated with N-Myr-GOA.Taken together, the results suggest that the myristoylation of HIV-1 gag seems to be responsible for both maturation of gag and acquisition of HIV-1 infectivity.
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