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Protection against oxidative stress by polyunsaturated fatty acids

Research Project

Project/Area Number 09672257
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

KIKUGAWA Kiyomi  School of Pharmacy, Tokyo University of Pharmacy and Life Science, Professor, 薬学部, 教授 (90120146)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsReactive oxyten species / Oxidative stress / Polyunsaturated fatty acid / Lipid peroxidation / Aging / Thiobarbituric acid test / 脂質過酸化 / 赤血球
Research Abstract

1. Use and limitation of thiobarbituric acid (TBA) test for lipid peroxidation was clarified.
2. Wistar male rats were fed a diet containing n-6 fatty acid-rich safflower oil or n-3 fatty acid-rich fish oil with an equal amunt of vitamin E for 6 weeks. n-3 Fatty acid content in erythrocyte membranes of rats fed fish oil was significantly higher than that of rats fed safflower oil. The degree of membrane lipid peroxidation and protein aggregation of ras fed fish oil was not significantly higher than that of rats fed safflower oil when the amounts of phospholipid hydroperoxides, TBA-reactive substances and detergent-insoluble protein aggregaates were measured. If a sufficient amount of vitamin E is supplied, n-3 fatty acid supplementation may give no undeseirable oxidative effects on rat erythrocytes in the circulation.
3. Wistar male rats were fed a diet containing safflower oil orfish oil with an equal amunt of vitamin E for 6 weeks. n-3 Fatty acid contents in lipids in brain, liver, lung, heazt, kidney, spleen, stomach ant testis were significantly higher than those of rats fed safflower oil. The degree of lipid peroxidation of the organs of ras fed fish oil was not significantly higher than that of rats fed safflower oil when the amounts of phospholipid hydroperoxides and TBA-reactive substances were measured. n-3 Fatty acid supplementation may give no undeseirable oxidative effects on rat organs.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 菊川清見: "生体内脂質の過酸化-測定法と正常値" 脂質栄養学. 6(1). 88-91 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyomi Kikugawa: "Use and limitation of thiabarbituric acid test ・・・" Recent Res.Develop.in Lipid Res.1. 73-96 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadamichi Inoue & Kiyomi Kigugawa: "Blus fluorescence generated during lipid oxidation ・・・・" Biol.Pharm.Bull.21(4). 319-325 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ken Ando, ・・・・Kiyomi Kikugawa et al: "Effect of n-3 fatty acid supplementation ・・・・・" Lipids. 33(5). 505-512 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 安藤 堅, ・・・ 菊川清見 ・・・: "ラット、赤血球膜の酸化的ストレスに対するn-3系多価不飽和脂肪酸摂取の影響" 脂質生化学研究. 40. 259-262 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadamichi Inoue : ・・ Kiyomi Kikugawa: "Specific determination of malonaldehyde ・・・・" J.Am.Oil Chem.Soc. 75(5). 597-606 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 奥山治美, 菊川清見編: "脂質栄養と脂質過酸化" 日本脂質栄養学会, 213 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyomi Kikugawa: "Lipid peroxidation in vivo-Measurement and normal level" Journal of Lipid Nutrition. 6(1). 88-91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyomi Kikugawa: "Use and limitation of thiobarbituric acid (TBA) test for lipid peroxidation" Recent Res.Devel, in Lipid Res.1. 73-96 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadamichi Inoue and Kiyomi Kikugawa: "Blue fluorescence generated during lipid oxidation of rat liver microsomes cannot be derived from malonaldehyde but can be from other aldehyde species" Biol.Pharm.Bull.21(4). 319-325 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ken Ando, Kunihide Nagata, Masatoshi Beppu, Kiyomi Kikugawa, Terue Kawabata, Kyoko Hasegawa and Masao Suzuki: "Effect of n-3 fatty acid supplementation on lipid peroxidation and protein aggregation in rat erythrocyte membranes" Lipids. 33(5). 505-512 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ken Ando, Kunihide Nagata, Masatoshi Beppu, Kiyomi Kikugawa, Terue Kawabata, Kyoko Hasegawa and Masao Suzuki: "Effect of n-3 fatty acid supplementation on lipid peroxidation and protein aggregation in rat erythrocyte membranes" Proceedings of Jpn.Conf.Biochem.Lipids. 40. 259-262 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadamichi Inoue, Ken Ando and Kiyomi Kikugawa: "Specific determination of malonaldehyde by N-methyl-2-phenyl-indole or thiobarbituric acid" J.Am.Oil Chem.Soc.75(5). 597-600 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyomi Kikugawa, Ken Ando, Rie Yoshida, Kunihide Nagata and Masao Suzuki: "Effects of dietary fats and polyphenolics on tissue lipid peroxidation" Proceedings of Jpn.Conf.Biochem.Lipids. 41 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Harumi Okuyama and Kiyomi Kikugawa ed.: "Lipid Nutrition Series 2, Lipid Nutrition and Lipid Peroxidation -Is lipid peroxidation in vivo damaging or protective? -" Gakkai Senta Kansai. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ken Ando, …, Kiyomi Kikugawa et al: "Effect of n-3 fatly acid supplementation on lipid Peroxidation and Protein ayyccgation in rat erythrocyte momleranes" Lipids. 33(5). 505-512 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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