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Antagonistic regulation of cell migration by epidermal growth factor and glucocorticoid.

Research Project

Project/Area Number 09672265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionSETSUNAN UNIVERSITY

Principal Investigator

ITO Fumiaki  SETSUNAN UNIVERSITY,FACUTY OF PHARMACEUTICAL SCIENCES,PROFESSOR, 薬学部, 教授 (80111764)

Co-Investigator(Kenkyū-buntansha) SHIBAMOTO Sayumi  SETSUNAN UNIVERSITY,FACUTY OF PHARMACEUTICAL SCIENCES,RESEARCH ASSOCIATE, 薬学部, 助手 (80178920)
HORI Takamitsu  SETSUNAN UNIVERSITY,FACUTY OF PHARMACEUTICAL SCIENCES,ASSISTANT PROFESSOR, 薬学部, 講師 (00199522)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsEpidermal growth factor / Glucocorticoid / Integrin / Cell migration / Extracellular matrix / Collagen / Cell adhesion / 接着斑
Research Abstract

Cell migration is a critical event in morphogenesis, tissue repair inflammatory reactions, and interactions in the immune system. A variety of growth factors such as epidermal growth factor (EGF) have been known to stimulate cell migration. In fact, we found that EGF stimulated cell migration of TMK-l, a cell line derived from a human gastric carcinoma. In this study, we have reported that the EGF-induced cell migration was inhibited if the cells were pretreated with dexamethasone, a synthetic glucocorticoid. Since interaction of cells with a variety of extracellular matrices such as collagen, fibronectin, and laminin is thought to be crucial for cell migration, we studied the effect of glucocorticoid and EGF on cell adhesion of TMK-1 to extracellular matrices. Dexamethasone increased cell adhesion to collagen typelV and laminin, but not to poly-L-lysine and fibronectin. In contrast, EGF did not affect cell adhesion to these extracellular matrices whether dexamethasone was present or n … More ot.
Cell-extracellular matrix interactions are mediated by a family of integriris, each of which is a cell-surface protein consisting of an alpha subunit noncovalently associated with a beta subunit. We then examined the expression level of a variety of alpha and beta subunits in TMK-1 cells which had been pretreated with dexamethasone or EGF.Dexamethasone enhanced the protein levels of both alpha 1 and beta 1 integrin subunits, and that of the alpha1beta 1 heterodimer. Further, flow cytometric analysis revealed that dexamethasone increased the expression of alpha 1and beta 1 subunits at the cell surface, whereas EGF increased expression of alpha 2 and beta 1 subunits at the cell surface. Antibodies against alpha 1 and beta 1 integrin subunits inhibited the increased cell adhesion seen in the presence of dexamethasone. These results suggest that glucocorticoid increased cell adhesion to the extracellular matrix via alpha 1beta1 integrin, and thereby antagonized EGF-induced cell migration. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Murakami Norie: "Antagonistic regulation of cell migration by epidermal growth factor and glucocorticoid in human gastric carcinoma cells" Journal of Cellular Physiology. 176. 127-137 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Murakami: "Antagonistic regulation of cell migration by epidermal growth factor and gllucocorticoid in human gastric carcinoma cells." J.Cell.Physiol.176. 127-137 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Murakami Norie: "Antagonistic regulation of cell migration by epidermal growth factor and glucocorticoid in human gastric cercinoma cells" Journal of Cellular Physiology. 176. 127-137 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Murakami Norie: "Antagonistic regulation of cell migration by epidermal growth factor and glucocorticoid in human gastric carcinomd cells" Journal of Cellular Physiology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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