| Project/Area Number |
09672284
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
医薬分子機能学
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| Research Institution | Faculty of Pharmaceutical Sciences, Kumamoto University |
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Principal Investigator |
OKAWARA Tadashi Faculty of Pharmaceutical Sciences, Kumamoto University, Associate Professor, 薬学部, 助教授 (60040325)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | 2'-5' oligoadenylate / nuclease / 1,3-dioxolane / adenyl phosphonate / bis-1,3-dioxolane / interferon / acyclic dinucleoside / protein synthetase inhibitor / 2',5'-オリゴアデニレート / ジオキソラン / ジヌクレオシド / 2'-5'-オリゴアデニレート |
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| Research Abstract |
2'-5' Oligo adenylate (2-5 A) found in the cell cultured extract from inferferon treated with virus is a strong inhibitor of protein synthetase. 2-5 A is considered to activate ribonuclease L (RNase L) to cleave m RNA resulting in inhibiting protein synthetase. Since then, preparation of wild type 2-5 A have been investigated by many group. However, 2-5 A is easily subject to cleavage of phosphate with nuclease. The half-value period has 2-3 minutes. If this weak point is overcome, 2-5 A will be a candidate of a potent anti-virus agent with immune activator. To prepare 2-5 A like acydlic oligoadenylate, the three following preparations were investigated.
1) Adenyl phosphonate was successfully prepared from 1, 3-dioxolane.
2) Dinucleosides were obtained from bis-1, 3-dioxolane by one-pot reaction.
3) Preparation of oligoadenylate from oligodioxolane was examined by substitution of phosphonate with sulfur linkage, but the intermediate dioxolane from diol and hydroxyaldehyde has not been isolated in large scale. Further preparation is being investigated.
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