| Project/Area Number |
09672292
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
医薬分子機能学
|
|---|
| Research Institution | Kobe Pharmaceutical University |
|---|
Principal Investigator |
ITO Masayoshi Kobe Pharm. Univ., Professor, 薬学部, 教授 (20068331)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
WADA Akimori Kobe Pharm. Univ., Associate Professor, 薬学部, 助教授 (80158683)
|
|---|
| Project Period (FY) |
1997 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Tricarbonyliron complex / 11Z-Retinal analog / 9-Substituted 11Z-retinal / Vision / Rhodopsin / Activation of signal transduction / 11Z-9位置換レチナール / β-イオノンアナログ鉄カルボニル錯体 / β-イオノンアナログ / 11Z-レチナ-ルアナログ / 9-ベンジルレチナ-ル / 9-フェニルレチナ-ル |
|---|
| Research Abstract |
Rhodopsin is the visual pigment which contains 11Z-retinal as a chromophore bound to the ε-amino group of the apoprotein lysine residue through a protonated Schiff base. Recently, it was suggested that an interaction between the 9-methyl group of retinal and the amino acid residues of apoprotein played an important role for exhibiting the biological function. In order to clarify the effect of 9-substituents in rhodopsin chromophore, we prepared the various retinal analogs, which have the different substituents at 9 position in retinal (Et, iso-Pr, iso-Bu, t-Bu, Ph, CHィイD22ィエD2Ph, CHィイD22ィエD2CィイD26ィエD2HィイD24ィエD2-OMe-p, 1-naphthylmethyl, 2-naphthylmethy and 3-indolylmethyl), according to our developed method using tricarbonyliron complex and their interaction with bovine opsin was investigated. The retinal analogs except for t-Bu, Ph and 1-naphthylmethyl bounded to apoprotein and the artificial rhodopsin analogs were produced. And also, it was found that the iso-Pr and benzly analogs exhibited about 70-80% activation of signal transduction compared to that of native rhodopsin.
|
