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Platelet activation induced by oxidized-LDL.-Involvement of CD36.

Research Project

Project/Area Number 09672345
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MATSUNO Kazuhiko  College of Medical Technology, Hokkaido Univ., Professor, 医療技術短期大学部, 教授 (70102332)

Co-Investigator(Kenkyū-buntansha) CHIBA Hitoshi  Medical hospital School of Med., Hokkaido Univ., Lec., 医学部・附属病院, 講師 (70197622)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsplatelets / CD36 / lipoprotein / LDL / oxidized-LDL / total cholesterol / CD62P / CD63 / カルシウム
Research Abstract

Oxidized-low density lipoprotein (ox-LDL), but not native LDL (n-LDL), induced platelet aggregation in washed platelet suspension, and induced the expression of CD62P and CD63 on platelets. N-LDL and native high density lipoprotein (n-HDL) inhibited ox-LDL-induced platelet aggregation. Monoclonal antibody against CD36 inhibited low concentration of ox-LDL-induced potentiation of thrombin-induced platelet aggregation. Ox-LDL-induced CD62P and CD63 expression was inferior in CD36 negative subjects than in CD36 positive subjects. Taken together, ox-LDL-induced platelet activation was considered to be partly mediated by CD36 on platelets.
We screened CD36 antigen on platelets and monocytes using flow cytometry in 1094 Japanese healthy volunteers, and studied the phenotype-genotype relationship of CD36 deficiency. The type I CD36 deficiency (negative CD36 on both platelets and monocytes) and type II CD36 deficiency (negative CD36 on only platelets) were found in 9(0.82%) and 59(5.39%), respectively. Of the 56 with phenotypic CD36 deficiency, 34(61%) had one or two reported mutations. No reported mutation was detected in 22 with typeII deficiency. The substituion of T for C at nt 478 constituted the major cause of type I deficiency. Total cholesterol level and LDL-cholesterol level were higher in CD36 negative subjects than in CD36 positive subjects. It is considered that the relationship LDL (and/or ox-LDL) and platelets was changed in CD36 negative subjects.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Takahashi, Y: "Significance of membrane glycoproteins in platelet interaction with oxidized low-density lipoprotein." Sem.Thrombos.Hemostas.24・3. 251-253 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 松野 一彦: "血栓形成に関わる血小板と酸化脂質の相互作用" 臨床血液. 39・2. 113-115 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 松野 一彦: "食事による血小板機能亢進改善の可能性" 日本臨床栄養学会雑誌. 20・2. 77-82 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukihiro Takahashi, et al.: "Significance of membrane glycoproteins in platelet interaction with oxidized low-density lipoprotein." Sem.Thrombos.Hemostas.24 (3). 251-253 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuhiko Matsuno, et al.: "Relationship between platelets and oxidized lipid in thrombus formation" JPN J.Clin.Hematol.39 (2). 113-115 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuhiko Matsuno: "Possibility of the improvement in platelet hyperacyivity by diet" J.JPN.Society Clin.Nutrition. 20 (2). 77-82 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi,Y.: "Significance of membrane glycoproteins in platelet interaction with oxidized low-density lipoprotein" Sem.Thrombos.Hemostas.24・3. 251-253 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 松野 一彦: "血栓形成に関わる血小板と酸化脂質の相互作用" 臨床血液. 39・2. 113-115 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 松野 一彦: "食事による血小板機能亢進改善の可能性" 日本栄養学会雑誌. 20・2. 77-82 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsuno,K., et al.: "Oxidized-low density lipoprotein (LDL) activates platelets via CD36." Thrombos.Haemostas.77 (Suppl). 260- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuno,K., et al.: "Heterogeneity of CD36 expression on human platelets." Thrombos.Haemostas.77 (Suppl). 341- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takahashi,Y., et al.: "Significance of membrane glycoproteins in platelet interaction with oxidized low density-lipoprotein" Sem.Thrombos.Haemostas.(in print). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松野一彦ら: "血栓形成に関わる血小板と酸化脂質の相互作用" 臨床血液. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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