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Studies on the function and the regulation of expression of platelet membrane protein GPIV (CD36)

Research Project

Project/Area Number 09672346
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionAsahikawa Medical College

Principal Investigator

HAYASHI Yukiko (1999-2000)  Asahikawa Medical College, Dept. of Medicine, Assistant Prof, 医学部, 講師 (50125407)

池田 久實 (1997-1998)  旭川医科大学, 医学部, 教授 (90091561)

Co-Investigator(Kenkyū-buntansha) KOHMURA Chikashi  Asahikawa Medical College, Dept. of Medicine, Instructor, 医学部, 助手 (40261408)
KAWABATA Isao  Asahikawa Medical College, Dept. of Medicine, Instructor, 医学部, 助手 (50195129)
林 由紀子  旭川医科大学, 医学部, 講師 (50125407)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
KeywordsPlatlet / Signal transduction / PKC / Arachidonic acid / CD36 / PUFA / 膜蛋白CD36 / 多価不飽和脂肪酸
Research Abstract

We have studied the effects of platelet membrane protein CD36 on the platelet signal transduction using CD36 defective platelets. One of the reported roles of CD36 is the transporter of unsaturated fatty acids. Compared to normal ones little characteristic responses of CD36 defective platelets have been reported. Arachidonic acid (AA), one of the unsaturated fatty acid, has pivotal role in platelet aggregation through its metabolite TxA2. We observed that under the decreasing store Ca2+ conditions CD36 defective platelets showed clear differences in the reactions where AA metabolites have more essential roles. We found that AA inhibits platelet aggregation induced by its metabolites TxA2. The IC50 of AA for normal platelets was 26.5±12.2 μM,and that for C36 defective platelets was 105±44 μM respectively. These results corroborate the role of CD36 as fatty acid transporter. This is the first report on the impaired functions of CD36 defective platelets. AA and other unsaturated fatty acids activate Ca2+ independent PKC in the cytosol independently of membrane lipids and cytosolic Ca2+ Physiological significances of the PKC isotypes have not been clearly understood. Hence the mechanisms of the inhibiting effects of AA were next studied. It was confirmed that AA inhibits PLCβ activation through serine phosphorylation by Ca2+ independent PKC.Consequently the Ca2+ dependent PKC closely connected to aggregation are not activated. Thus PKC isotypes are linked to each other leading to the physiologically proper responses of platelets. More important point is that AA itself regulates reactions induced by its descendants through the PKC isotypes.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y,Hayashi: "Preparation of discoid washed platelets by differential centrifugation"Uinia Chimica Acta. 275. 99-105 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.Kohmura: "Detection of Activated Platelets by Flow Cytometry"臨床病理. 47. 447-452 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.Kohmura: "Flour Cytometric Evaluation of Platelet Activation-Sncrease of Surface Antigen Expression and Sts Reduction by Anti-platelet Agonts"Cytometry Research. 9. 67-72 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Hayashi, S.Takenaka, C.Kohmura, H.Ikeda.: "Preparation of discoid washed platelets by differential centrifugation"Clinica Chimica Acta. 275. 99-105 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Hayashi, H.Ikeda: "Inhibition of platelet aggregation by arachidonic acid"Japanese J.Thrombo. and Hemost.. 10. 369 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Hayashi, C.Kohmura, H.Ikeda: "Uptake of arachidonic acid and its inhibitory effects in CD36 defective platelets"Japanese J.Thrombo. and Hemost.. 9. 300 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Hayashi, C.Kohmura, H.Ikeda: "Relationship between released prostaglandines and irreversible aggregation during preparation of washed platelets."Japanese J.Thrombo. and Hemost.. 8. 275 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 林 由紀子、池田久實: "アラキドン酸による血小板凝集阻害反応"日本血栓止血学会誌. 10巻、5号. 369 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 幸村近、池田久實: "フローサイトメトリーを用いた抗血小板薬の効果判定"日本血栓止血学会誌. 10巻、5号. 378 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 幸村近、林由紀子、池田久實: "フローサイトメトリーを用いた活性化血小板の検出法"臨床病理. 47巻、5号. 447-452 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 幸村近、池田久實: "フローサイトメトリーによる血小板活性化の評価"Cytometry Reseach. 9(2). 67-72 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 幸村近、井出夏代、林由紀子、池田久實: "血小板の形態変化とGPIIb/IIIa活性化"臨床病理. 47巻補冊. 294 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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