| Project/Area Number |
09672348
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
FUNATO Tadao Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (70165455)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHINO Atsushi Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (60241600)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | drug resistance / leukemia / real-time RT-PCR / Multidrug reistance / cytosine arabinoside / 多剤耐性遺伝子(MDR) / 薬剤耐性遺伝子 / 遺伝子診断 |
|---|
| Research Abstract |
The failure of chemotherapy to eradicate human leukemic cells is often due to the development of drug resistance. MDR (multidrug resistance) whose one form of drug resistance results from a decreased intracellular accumulation of the drugs, most often mediated by the overexpression of P-glycoprotein. Multidrug-related protein (MRP) also related to pump function of cell membrane in acute leukemia. Decreased deoxycytidine kinase (dCK) gene was observed in cytosine arabinoside resistant leukemia cells (ara-C) which we have established. We have developed the new quantitative assay based on real-time reverse transcriptase polymerase chain reaction (RT-PCR) to mesure expression of drug-resistance related genes such as MDR-1, MRP, and dCK in clinical samples. These results indicates that real-time RT-PCR system is a releable method to quantitatively determine drug resistant genes expression, it may be to predict responsiveness to chemotherapy by using this technique.
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