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Quantification of rouleaux formation by red cells in whole blood

Research Project

Project/Area Number 09672359
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKOCHI MEDICAL SCHOOL

Principal Investigator

IRIMAJIRI Akihiko  KOCHI MEDICAL SCHOOL, Department of Physiology, Professor, 医学部, 教授 (30025605)

Co-Investigator(Kenkyū-buntansha) IMAMURA Jun  KOCHI MEDICAL SCHOOL, Dept. of Laboratory Medicine, Assistant Professor, 医学部・附属病院, 講師 (30232614)
ANDO Tomonari  KOCHI MEDICAL SCHOOL, Department of Physiology, Research Associate, 医学部, 助手 (20222789)
RAICU Valerica  KOCHI MEDICAL SCHOOL, Department of Physiology, Research Associate, 医学部, 助手 (20304678)
ICHINOWATARI Takako  KOCHI MEDICAL SCHOOL, Department of Physiology, Instructor, 医学部, 教務職員 (90145152)
竹内 俊二  高知医科大学, 医学部, 助手 (50188158)
渡辺 牧夫  高知医科大学, 医学部・附属病院, 助手 (00220921)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordswhole blood / impedance / permittivity / ESR / rouleaux formation / red cell / aggregation / laboratory medicine / 赤血球凝集 / 病態検査法 / 血小板
Research Abstract

To develop a quick method of obtaining a practical index replaceable for the conventional erythrocyte sedimentation rate (ESR), we examined the possibility of applying the dielectric spectroscopy to the measurement of rouleaux formation in whole blood. The main findings follow.
(1) Rouleaux formation is a key determinant of the ESR.
(2) The degree of rouleaux formation in whole blood is readily detectable through measurements of relative permittivity (ε) for the blood at low frequencies (10-100 kHz).
(3) We fabricated a rotary cell (I.e. a sample housing with a pair of electrodes as the sensor) with which to measure c while changing the shear stress to be exerted on the blood sample.
(4) Upon varying the shear rate stepwise, the low-frequency level of ε traced stepwise decreases with increasing shear rate and showed a mirror image with decreasing shear rate.
(5) These stepwise changes in whole blood s could be correlated with ESR.
(6) Another approach was also pursued which employed a pair of open-ended coaxial probes set at both ends of a blood-filled, vertical, Perspex column. This method enabled a simultaneous determination of ESR and low-frequency ε. Preliminary experiments indicated that these two separate indices correlated well with each other.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 入交昭彦: "全血の誘電挙動からみた赤血球凝集(連銭形成)"生物工学会誌. 79,(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Raicu,V.: "Dielectric dispersion of biological matter : Model combining Debye-type and 'universal' responses"Physical Review E. 60. 4677-4680 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Raicu,V.: "Dielectric properties of rat liver in vivo : A noninvasive approach using an open-ended coaxial probe at audio/radio frequencies"Bioelectrochemistry & Bioenergetics. 47. 325-332 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Raicu,V.: "Dielectric properties of rat liver in vivo : Analysis by modeling hepatocytes in the tissue architecture"Bioelectrochemistry & Bioenergetics. 47. 333-342 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Raicu,V.: "Multifrequency method for dielectric monitoring of cold-preserved organs"Physics in Medicine & Biology. 45,(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Raicu,V.: "A quantitative approach to the dielectric properties of the skin"Physics in Medicine & Biology. 45. L1-L4 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A. Irimajiri, V. Raicu, J. Imamura: "Red cell aggregation (rouleaux formation) as viewed from dielectric behavior of whole blood"Seibutsu-Kogaku Kaishi. 78(5)(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A. Irimajiri, M. Ando, S. Takeuchi: "Low-frequency permittivity can report the degree of rouleaux formation in whole blood"Proc. 33ィイD1rdィエD1 Int. Congress of Physiol. Sci.. 10. 106 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] V. Raicu: "Dielectric dispersion of biological matter : Model combining Debye-type and 'universal' responses"Phys. Rev. E. 60. 4677-4680 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] V. Raicu, T. Saibara, A. Irimajiri: "Dielectric properties of rat liver in vivo : A noninvasive approach using an open-ended coaxial probe at audio/radio frequencies"Bioelectrochem. Bioenerget.. 47. 325-332 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] V. Raicu, T. Saibara, H. Enzan, A. Irimajiri: "Dielectric properties of rat liver in vivo : Analysis by modeling hepatocytes in the tissue architecture"Bioelectrochem. Bioenerget.. 47. 333-342 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] V. Raicu, T. Saihara, A. Irimajiri: "Multifrequeney method for dielectric monitoring of cold-preserved organs"Phys. Med. Biol.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] V. Raicu, N. Kitagawa, A. Irimajiri: "A quantitative approach to the dielectric properties of the skin"Phys. Med. Biol.. 45. L1-4 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Raicu,V.: "Dielectric properties of rat liver in vivo:a noninvasive approach using an open-ended coaxial probe at audio/radio freguencies" Bioelectrochemistry or Bioenergetics. 47. 325-332 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Raicu,V: "Dielectric properties of rat liver in vivo:analysis by modeling hepatocytes in the tissue architecture" Bioelectrochemistry or Bioenergetics. 47. 333-342 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] MATSUOKA,R.: "Dielectric dispersion of protein-bound water" Japanese Journal of Physiology. 47. S50 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2021-04-07  

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