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An attempt to develop a biodosimeter for detecting low dose and low dose rate radiation

Research Project

Project/Area Number 09680521
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionOsaka University

Principal Investigator

NAKAJIMA Hiroo  Osaka University Faculty of Medicine, Assistant Professor, 医学部, 助手 (20237275)

Co-Investigator(Kenkyū-buntansha) NOMURA Taisei  Osaka University Faculty of Medicine, Professor, 医学部, 教授 (90089871)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsSCID / Cell culture / Mouse / Biodosimater / Double strand break / DNA repair / Low dose radiation / Potentially lethal damage
Research Abstract

We attempted to develop a new biodosimeter to detect the low dose and low dose rate radiation.
Normal (wild type cells) and double-strand break repair deficient murine cells (SCID cells) were exposed to gamma-rays at low and high dose rates with dose fractionation and with different recovery times, in order to examine the dose rate effectiveness and repair of sublethal and potentially lethal damage.In addition, 5-fluorouracil (5-FU), a commonly used drug for cancer therapy, was a]so used, because it produces double-strand breaks.
SOlD cells showed deficiency in repairing DNA damage induced by 5-FU or gamma-rays even after dose fractionation and after 24h recovery periods, while the wild type cells showed an apparent repair ability on DNA damage after 5-FU treatment or gamma-rays exposure.
We conclude that the SCID cell is useful system as a biodosimeter for detecting the damage of low dose radiation.
Now, we are trying to establish a method to count the number of double-strand break points in a SCID cell.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Nomura,T.,et al.: "Induction of cancer,actinic keratosis,and specific p53 mutation by UVB light in human skin maintained in severe commbined immunodeficient mice." Cancer Res.57. 2081-2084 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaguchi,T.,et al.: "Consecutive maintenance of human solitary and hereditary colorectal polyps in SCID mice and K・ras mutations." Cancer Detection and Prevention. 21. 148-157 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakajima,H.,et al.: "Radionuclides carved on the annual rings of a tree near Chernobyl." Health Physics. 74. 265-267 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Li,L.Y.,et al.: "Dose rate effectiveness and potentially lethal damage repair in normal and double・strand break repair deficient murine cells by γ・rays and 5・fluororacil." Cancer Letters. 123. 227-232 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukuda,K.,et al.: "Morphology and function of human benign tumors and normal thyroid tissues maintained in severe combined immunodeficient mice." Cancer Letters. 132. 153-158 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nomura, T., et al.: "Induction of cancer, actinic keratosis, and specific p53 mutation by UVB light in human skin maintained in severe commbined immunodeficient mice." Cancer Res.57. 2081-2084 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaguchi, T., et al.: "Consecutive maintenance of human solitary and hereditary colorectal polyps in SCID mice and K-ras mutations." Cancer Detection and Prevention. 21. 148-157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakajima, H., et al.: "Radionuclides carved on the annual rings of a tree near Chernobyl." Health Physics. 74. 265-267 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Li, L.Y., et al.: "Dose rate effectiveness and potentially lethal damage repair in normal and double strand break repair deficient murine cells by gamma rays and 5 fluorouracil." Cancer Letters. 123. 227-232 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukuda, K., et al.: "Morphology and function of human benign tumors and normal thyroid tissues maintained in severe combined immunodeficient mice." Cancer Letters. 132. 153-158 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nomura,T.,et al.: "Induction of cancer.actinic keratosis,and specific p53 mutation by UVB light in human skin maintained in severe commbined immunodeficient mice." Cancer Res.57. 2081-2084 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawaguchi,T.,et al.: "Consecutive maintenance of human solitary and hereditary colorectal polyps in SCID mice and K-ras mutations." Cancer Detection and Prevention. 21. 148-157 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakajima,H.,et al.: "Radionuclides carved on the annual rings^n of a tree near Chernobyl" Health Physics. 74. 265-267 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Li,L.,et al.: "Dose tate effectiveness and potentially lethal damage repair in normal and double strand break repair deficient murine cells by γ rays and 5-fluorouracil." Cancer Letters. 123. 227-323 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukuda,K,et al.: "Morphology and function of human benign tumors and normal thyroid tissues maintained in severe combined immunodeficient mice." Cancer Letters. 132. 153-158 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nomura,T., et al.: "Induction of cancer,actinic keratosis,and specific p53 mutation by UVB light in human skin maintained in severe commbined immunodeficient mice." Cancer Res.57. 2081-2084 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawaguchi,T., et al.: "Consecutive maintenance of human solitary and hereditary colorectal polyps in SCID mice and K-ras mutations." Cancer Detection and Prevention. 21. 148-157 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakajima,H.et.al.: "Radionuclides carved on the annual rings of a tree near Chernolobyl" Health Physics. 74. 265-267 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Li,L., et.al.: "Dose-rate effectiveness and potentially lethal damage repair in normal and double-strand-break repair deficient murine cells by γ rays and 5-fluorouracil." Cancer Letters. 123. 227-323 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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