Competitive analysis of radiation induced gene expression between thyroid cell and fibroblast
| Project/Area Number |
09680529
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAMBA Hiroyuki Nagasaki University School of Medicine, Associate Professor, 医学部, 助教授 (80237635)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUYAMA Toshifumi Nagasaki University School of Medicine, Professor, 医学部, 教授 (30165922)
YAMASHITA Shunichi Nagasaki University School of Medicine, Professor, 医学部, 教授 (30200679)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Radiation / Thyroid gland / Intra-cellular signal transdation / Cloning |
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| Research Abstract |
1) Thyroid gland is known to be one of radiation sensitive organs. High incidence of thyroid tumor is observed in the screening of HIBAKUSHA in Hiroshima and Nagasaki or Chernobyl region. To examine the difference of radiation sensitivity, we comparatively analyzed JNK activity following exposure to radiation between thyroid cells and fibroblasts. We found the JNK activity was 2-3 fold induction in thyroid cells following radiation exposure (2-5Gy), but no induction in fibroblasts. Further study revealed the radiation-induced JNK activity was mcdiated by protein kinase C.The paper described the results was published in BBRC (244, 1998).
2) To investigate the genes of which expressions are induced by irradiation, we analyzed the repertoire of the genes using Serial Analysis Gene expression method. 12016 tags from no- exposed cell and 14020 tags from 10 Gy exposed cells have been analyzed. We further continue to analyze the tags of exposed cells and non-exposed cells.
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Report
(3 results)
Research Products
(8 results)
