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Study of gene level for effect of smoking on alveolar macrophage function and lung metastasis of tumor.

Research Project

Project/Area Number 09680537
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionKyoto Sangyou University

Principal Investigator

TAKEUCHI Minoru  Kyoto Sangyou University, Biotechnology, Professor, 工学部, 教授 (70257773)

Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordssmoking / alveolar macrophage / tumor / DNA / mRNA / metastasis / mRNA / 転移 / 腫瘍 / 抗原提示能 / 表面抗原 / 高タールタバコ / 低タールタバコ / サイトカイン
Research Abstract

In previous studies, the effects of smoking on the gene level of alveolar macrophages(AM) immunological functions and lung metastasis of tumor cells are not well defined. In the present study, we investigated the effects of smoking on the gene level of alveolar macrophages immunological functions and lung metastasis of tumor cells in mice. Female C57BL/6 mice were exposed to 20 cigarettes (Coresta Monitor No.2) / day during 10 days using Hamburg II smoking machine. After 10 days, mice were sacrificed. The AM were obtained by brochoalveolar lavage (BAL). The antigen presenting activity of AM was assayed by mixed lymphocyte reaction (MLR). The reactive oxygen species productions in AM were analyzed by flow cytometry using hydroethidine and dichlorofluorescen (DCFH). The expression of surface antigens (Class II and B7-1) of AM were analyzed by flow cytometry. The expressions of IL- 1 β and TNF- α mRNA of gene in AM were analyzed by RT-PCR.The damages of DNA genes were analyzed by 8-OHdG.T … More he lung metastases of tumor cells were assayed by using Lewis lung carcinoma cells(LL/2). The antigen presenting activity of AM was significantly (p<0.01) decreased in smoked mice compared with non-smoked mice. The O_2^- production of AM was significantly (p<0.01) increased in smoked mice, whereas the H_2O_2 production of AM was not difference in both groups. The 8-OHdG levels of DNA in lung tissue were increased in smoked mice. The Class II and B7-1 antigen positive cells in AM were significantly(p<0.01) decreased in smoked mice compared with non-smoked mice. IL-1 β and TNF- α mRNA genes expressions in AM were decrease in smoked mice as compared to non-smoked mice. The metastases to lung of LL/2 tumor cells were promoted in smoked mice compared with non-smoked mice. These results suggest that the antigen presenting activity, expressions of Class II and B7-1 antigens of the AM may be impaired and DNA gene in lung tissue also may be damaged by O_2^- but not H_2O_2 from AM in smoked mice. Less

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] M.Takeuchi et.al: "Effect of soft X-ray irradiation on NK cell activity and the percentage of asialo GM-1 positive cells in spleen cells of mice"J.Vet.Med.Sci. 59. 413-414 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikuniya et al: "Quantitative evaluation of the IL-1β and IC-1 receptor antagonist obtained from BALF macrophages in patients with interstitial lung diseases."Sarcoidosis Vasuculites and Diffuse Lung Diseases. 14. 39-45 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takeuchi et al: "The number of interleukin-1 (IL-1) receptor on lung fibroblasts in patients with idiopathic pulmonary fibrosis."Respiration. 6. 236-241 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Nagai et al: "Angiotensin II receptor on BALF macrophages from japanese patients with active sarcoidosis."Sarcoidosis Vasuculites and Diffuse Lung Diseases. 16. 67-74 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takeuchi: "Suppressive effect of smoking on natural killer (NK) cell activity and it's suppressive mechanism by alveolar macrophages (AM) in smokers."The bullentin of the research institute for modern physical education Kyoto Sangyo University. 8. 49-56 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikuniya et al: "Significance of the interleukin-1 receptor antagonist/interleukin-1 β ratio as a prognostic factor in pulmonary sarcoidosis."Respiration. 67. 389-396 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Rushan Lu et al: "tobacco : The Growing Epidemic"Springer. 998 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.K.Varma et al: "Tobacco Counters Health"Macmillan India Ltd.. 197 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takeuchi and H.Shibata: "Effect of soft x-ray irradiation on NK cell activity and the percentage of asialo GM1-positive cells in spleen cells of mice."J.Vet.Med.Sci.. 59. 413-414 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikuniya, S.Nagai, T.Shimoji, M.Takeuchi, K.Morita, T.Mio, N.Satake and T.Izumi: "Quantitative evaluation of the IL-1β and IL-1 receptor antagonist obtained from BALF macrophages in patients with intersitial lung diseases."Sarcoidosis Vasuculites and Diffuse Lung Diseases. 14. 39-45 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takeuchi, S.Nagai, T.Tsutsumi, T.Mio and T.Izumi: "The number of interleukin-1 (IL-1) receptor on lung fibroblasts in patients with idiopathic pulmonary fibrosis."Respiration. 6. 236-241 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Nagai, M.Takeuchi, K.Morita, T.Mikuniya, N.Satake, T.Mio and T.Izumi: "Angiotensin II receptor on BALF macrophages from japanese patients with active sarcoidosis."Sarcoidosis Vasuculites and Diffuse Lung Diseases. 16. 67-74 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takeuchi: "Suppressive effect of smoking on natural killer (NK) cell activity and it's suppressive mechanism by alveolar macrophages (AM) in smokers."The bullentin of the research institute for modern physical education Kyoto Sangyo University. 8. 49-56 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikuniya, S.Nagai, M.Takeuchi, T.Mio, Y.Hosono, H.Miki, M.Shigematsu, K.Hamada and T.Izumi: "Significance of the interleukin-1 receptor antagonist/interleukin-1β ratio as a prognostic factor in pulmonary sarcoidosis."Respiration. 67. 389-396 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takeuchi, S.Nagai and T.Izumi: "Inhibition of natural killer cell activity by alveolar macrophages in smokers."Tobacco : The Growing Epidemic. (Rushan Lu, Judith Mackay, Shiru Niu and Richard Peto eds.). Springer.. 135-136 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takeuchi, A.Nakajima, K.Yoshikawa, M.Shinya, H.Asada, M.Yoshimura, C.Tsukano, S.Nagai and T.Izumi: "Effect of smoking on immunological functions of alveolar macrophages in mice."Tobacco Counters Health (A.K.Varma ed), Macmilan Ltd.. 168-171 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mikuniya et al: "Significance of the interleukin-1 receptor antagonist/linterleukin-1 βratio as a prognostic factor in pulmonary sarcoidosis"Respiration. 67. 389-396 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Rushan Lu, et al: "tobacco : The Growing Epidemic"Springer. 998 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.K.Varma et al: "Tobacco Counters Health"Macmillan India Ltd.. 197 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M. Takeuchi et al: "The Number of inter leukin l Receetrs on lung fibroblats in patients with idsopathic pulmsnary fibrosis"Respirafion. 66. 236-241 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sonoko Nagai et al: "Angiotensin II receptur on BALF macrophages from Japanese Pareents with acfive sarcsidosis"Sarcoidcsis Vasc Diffuse Lung Dis. 16. 67-74 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Minoru Takeuchi: "Suyrressive effest of Smoksng on NK cell activity and it's suppressive meevanism by AM in smohons"The Bulletom of the research inslifute for modern Physical eclucahon Kyoto sengyo Universitiy. 8. 49-56 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Minoru Takeuchi et al: "Effect of Soft-ray irradiation on NK all activity and the perontage of asialo GM-1 positive cells in spleen cells of mice" J.Vet.Med.Sci.59. 413-414 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Mikuniya et al: "Quantitative evaluation of the IC-1P and IC-1 recept for antagonist obtained from BALF macrophagesin patients with interstitial lung diseases." Sarcoidosis Vasuculites and Diffuse Lung Diseases. 14. 39-45 (1997)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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