| Project/Area Number |
09680613
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Chiba University Graduate School of Medicine |
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Principal Investigator |
KIMURA Sadao Chiba University Graduate School of Medicine, Department of Biochemistry and Molecular Pharmacology, Proffesor, 大学院・医学研究科, 教授 (40134225)
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| Co-Investigator(Kenkyū-buntansha) |
MOROI Kayoko Chiba University Graduate School of Medicine, Department of Biochemistry and Mol, 大学院・医学研究科, 助手 (80110352)
NISHIYAMA Mariko Chiba University Graduate School of Medicine, Department of Biochemistry and Mol, 大学院・医学研究科, 助手 (00092081)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Endothelin / Endothelin receptors / Endothelin receptor antagonists / Desensitization / Binding experiment / Regulators of G protein signaling / G蛋白質共役受容体キナーゼ |
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| Research Abstract |
(1) [^<25>I]endothelin(ET)-l binding and functional properties of CHO cells stably coexpressing ETA and ETB receptors were studied. The results provide a new insight into relationship between ET receptors and their ligands, demonstrating that ET-1 displaced from ETB receptors by ETB receptor antagonists is trapped by ETA receptors and causes ETA receptor-mediated responses. (2) RGS (regulator of G protein signaling) genes which present in heart and smooth muscle were transiently expressed into cells and their suppression activities of ET-1-mediated responses were evidenced.
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