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Mechanism of allograft rejection : molecular biological analysis of delf/nonself recognition

Research Project

Project/Area Number 09680636
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionOsaka Medical College

Principal Investigator

YOSHIDA Ryotaro  Osaka Medical College Physiology, Associate Professor, 医学部, 助教授 (10124760)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsself / nonself / cytotoxic T cells / macrophages / apoptosis / Fas / FasL / perforin / histocompatibility antigen / skin allograft rejection / NK細胞 / 移植片拒絶 / パ-フォリン / Fas ligand
Research Abstract

When BALB/c skin or Meth A fibrosarcoma cells (BALB/c origin) was transplanted to C57BL/6 mice, two types of cytotoxic cells [i.e., CD8^+ cytotoxic T lymphocytes (CTLs) and macrophages (Mphis)] infiltrated into the rejection site. The Mphis lysed the allografted skin components or the tumor cells but were inert toward donor-type lymphoblasts. By contrast, the CTLs were inactive against the allografts, whereas they were cytotoxic against the donor-reated lymphoblasts. The infiltration of Mphis preceded the infiltration of CTLs by several days during the course of rejection. As nobody has expected that there were two types of cytotoxic cells infiltrating into the rejection site, most of the previous investigators have concluded that T lymphocytes are the effector cells.
The allograft-induced Mphis induced the apoptotic death of allografted cells through a third mechanism, one other than the Fsa/FasL- and perform-based pathways and the mice rejected the allografi on days 9-11 after transplantation. The Mphi population in bulk infiltrates from the rejection site was cytotoxic against allografted, but not self, skin components even in the absence of T cells. In addition, the Mphi-mediated cytotoxicity against the allograft was antibody-independent. Therefore, we prepared a cDNA library from allograft-induced Mphis and established several clones of monoclonal antibodies to the Mphis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] R.Yoshida et al.: "Two Distinct Populations of Primary Cytotoxic Cells Infiltrating into Allografted Tumor" Microbiol.Immunol.41. 149-159 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Yoshida et al.: "Ca2+-dependent, Fas- and Perforin-independent Apoptotic Death of allografted Tumor Cells by a Type of Activated Macrophage." J.Immunol.159. 15-21 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Usiho-Umeda and R.Yoshida: "Mechanisims of Allografted Tumor Rejection : The Roles of T Cells in Allograft Rejection Mediated by a Type of Bone Marrow-derived Macrophage." Int.Immunol.41. 981-990 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Yamamoto et al.: "Cellular Basis of Skin Allograft Rejection in Mice : Specific Lysis of Allogeneic Skin Components by Non-T Cells." Transplantation. 65. 818-825 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Yoshida et al.: "Tracing the In Vivo Distribution and Dynamics of Cancer Cells in Mice by Luminescence of Aequorin-expressing Transformants." Biochem.Biophys.Res.Commun.242. 659-662 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Yoneda, and R.Yoshida: "A Role of T Cells in Allografted Tumor Rejection : IFN-γ Released from T Cells Is Essential for Induction of Effector Macrophages in The Rejection Site." J.Immunol.160. 6012-6017 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 吉田 龍太郎: "ハイオディフェンスシリーズIIマクロファージと生体防御-新しい研究の展開-" 菜根出版, 22 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Yoshida: "Allograft-indeced Macrophage : A Novel Type of Activated Macrophage Involved in Allograft Rejection" Research Trends in press,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Yoshida, A.Matsuura, K.Einaga, Y.Ushio, N.Yamamoto, and Y.Yoneda: "Two Distinct Populations of Primary Cytotoxic Cells Infiltrating into Allografted Tumor Rejection Site : Infiltration of Macrophages Cytotoxic against Allografted Tumor Preceds That of Multiple Sets of Cytotoxic T Lymphocytes with Distinct Specificity to Alloantigens." Microbiol.Immunol.41. 149-159 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Yoshida, A.Sanchez-Bueno, N.Yamamoto, and K.Einaga-Naito.: "Ca^<2+>-dependent, Fas-and Perforin-independent Apoptotic Death of allografted Tumor Cells by a Type of Activated Macrophage." J.Immunol.159. 15-21 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Ushio-Umeda, and R.Yoshida: "Mechanisms of Allografted Tumor Rejection : The Roles of T Cells in Allograft Rejection Mediated by a Type of Bone Marrow-derived Macrophsge." Microbiol.Immunol.41. 981-990 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Yamamoto, K.Einaga-Naito, M.Kuriyama, Y.Kawada, and R.Yoshida: "Cellular Basis of Skin Allograft Rejection in Mice : Specific Lysis of Allogeneic Skin Components by Non-T Cells." Transplantation. 65. 1-8 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Yoneda, and R.Yoshida: "A Role of T Cells in Allografted Tumor Rejection : IFN-gamma Released from T Cells Is Essential for Induction of Effector Macrophages in The Rejection Sites." J.Immunol.160. 6012-6017 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Yoshida, A.Sanchez-Bueno, and F.I.Tsuji.: "Tracing the in Vivo Distribution and Dynamics of Cancer Cells in Mice by Luminescence of Aequorin-expressing Transformants." Biophys.Res.Commun.242. 659-662 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Yamamoto et al.: "Cellular Baasis of Skin Allograft Rejection in Miee : Specific Lysis of Allogeneic Skin Components by Non-TCells." Transplantation. 65. 818-825 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] R.Yoshida et al.: "Tracing the In Vivo Distribution and Dynamics of Cancer Cells in Mice by Luminescence of Aequorin-expressing Transformants." Biochem.Biophys.Res.Commun.242. 659-662 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Yoneda,and R.Yoshida: "A Role of T Cells in Allografted Tumor Rejection : IFN-γ Released from T Cells Is Essential for Induction of Effector Macrophages in The Rejection Site." J.Immunol.160. 6012-6017 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] R.Yoshida et al.: "IFN-γ-and Cell-to-cell Contact-dependent Cytotoxicity of Allograft-induced Macrophages against Syngeneic Tumor Cells and Cell Lines." J.Immunol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] R.Yoshida: "Current Trends in Immunology" Allograft-induced Macrophage : ANovel Type of Activated Macrophage Involved in Allograft Rejection (in press), (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] R.Yoshida, et al.: "Two Distinct Populations of primary Cytotoxic Cells Infiltrating into Allografted Tumor" Microbiol.Immunol.41. 149-159 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] R.Yoshida, et al.: "Ca2+-dependent,Fas-and Perforin-independent Apoptotic Death of allografted Tumor Cells by a Type of Activated Macrophage." J.Immunology. 159. 15-21 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Ushio-Umeda and R.Yoshida: "The Roles of CD4^T and CD8^T T Cells in Allografted Tumor Rejection Mediated by a Type of Bone Marrow-derived Macrophage." Microbiol.Immunol.41. 981-990 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Yamamoto, et al.: "Cellular Basis of Skin Allograft Rejection in Mice:Specific Lysis of Allogeneci Skin Components by Non-TCells." Transplantation. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] R.Yoshida, et al.: "Tracing the In Vivo Distribution and Dynamics of Cancer Cells in Mice by Luminescence of Aequorin-expressing Transformants." Biochem.Biophys.Res.Commun. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Yoneda,and R.Yoshida: "A Role of T Cells in Allografted Tumor Rejection:IFN-γ Released from T Cells Is Essential for Induction of Effector Macrophages in The Rejection Site." J.Immunol.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉田龍太郎: "免疫'97" Allograft-induced Macrophage, 130-137 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉田龍太郎: "マクロファージと生体防御-新しい研究の展開-" マクロファージと移植片拒絶, 159-181 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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