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Enzymatic Properties and Physiological Role of Membrane bound-Prostaglandin E Synthase

Research Project

Project/Area Number 09680637
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionUniversity of East Asia (1999-2000)
Osaka Bioscience Institute (1997-1998)

Principal Investigator

WATANABE Kikuko  University of East Asia, Department of Life Science and Technology, Professor, 工学部, 教授 (90211672)

Co-Investigator(Kenkyū-buntansha) OHISHI Kenji  University of East Asia, Department of Life Science and Technology, Professor, 工学部, 教授 (10152042)
MATSUMURA Hitoshi  Osaka Medical College, Department of Neuropsychiatry, Assistant Professor., 精神神経科, 講師 (50173886)
ITO Seiji  Kansai Medical University, Department of Medical Chemistory, Professor, 医化学教室, 教授 (80201325)
早石 修  (財)大阪バイオサイエンス研究所, 第2研究部, 名誉所長 (40025507)
鈴木 登志子  徳島大学, 第一解剖学教室, 助手
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsProstaglandin / Membrane-bound enzyme / PGE synthase / Purification of Enzyme / Cloning / 酵素 / 精製 / 膜結合 / グルタチオン
Research Abstract

E series of prostaglandin (PG)s were first discovered in sheep seminal vesicles. PGE_2 is widely distributed in various organs, and exhibits various biological activities such as smooth muscle dilatation/contraction, Na^+ excretion, body temperature regulation, inhibition of gastric acid secretion, and inhibition of immune responses. PGE synthase (EC.5. 3. 99. 3.) catalyzes the conversion of PGH_2 to PGE_2. In 1997, we reported that PGE synthase activity is widely distributed in the microsomal fractions of rat organs. Most of the PGE synthase activities in these organs absolutely required glutathione (GSH). In contrast, the enzyme activity in the heart, spleen, and uterine microsomes required SH-reducing reagents including dithiothreitol (DTT), GSH, or β- mercaptoethanol (β-Mer), but the requirement for its catalytic activity was not specific for GSH.We purified the GSH specific PGE synthase from bovine heart microsomes to apparent homogeneity, and partially purified the GSH unspecific PGE synthase from sheep seminal vesicle microsomes. We examined their molecular and catalytic properties.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Nishizawa,M.,Watanabe,K.,and Ito,S.: "Close Kinship of Human 20a-Hydroxysteroid Dehydrogenase Gene with Three Aldo-keto Reductase Genes."Genes Cells.. 5・2. 111-125 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki-Yamamoto,Watanabe,K.and Ishimura,K.: "Immunocytochemical localization of lung-type prostaglandin F synthase in the rat spinal cord"Brain Res.. 877・2. 391-395 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Dieter P,Watanabe K,and Kamionka S.: "Functional coupling of cyclooxygenase 1 and 2 to discrete prostanoid synthases in liver macrophages."Biochem Biophys Res Commun. 276・2. 488-492 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 渡部紀久子,山本(鈴木)登志子: "現代化学増刊38プロスタグランジン研究の最前線PGE合成酵素およびPGF合成酵素"東京化学同人. 224(5) (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe, K., Kurihara, K., Tokunaga, Y., and Hayaishi, O.: "Two types of microsomal prostaglandin E synthase-Glutathione-dependent and -independent prostaglandin E synthases-"Biochem. Biophys. Res. Commun.. 235. 148-152 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki, T., Watanabe, K., Kanaoka, Y., Sato, T., and Hayaishi, O.: "Induction of hematopoietic prostaglandin D synthase in human megakaryocytic cells by phorbol ester."Biochem. Biophys. Res. Commun.. 241. 288-293 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki, K., Fujii, Y., Miyano, M., Chen, L-Y, Takahashi, T., and Watanabe, K.: "cDNA Cloning, Expression, and Mutagenesis Study of Liver-type Prostaglandin F Synthase."J.Biol. Chem.. 274. 241-248 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe, K., Kurihara, K., and Suzuki, T.: "Purification and Characterization of Membrane-bound Prostaglandin E Synthase from Bovine Heart."Biochim. Biophys. Acta.. 1439. 406-414 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikeda, S., Okuda-Ashitaka, E., Masu, Y., Suzuki, T., Watanabe, K., Nakao, M., Shingu, K., and Ito S.: "Cloning and characterization of two novel aldo-keto reductases (AKR1C12 and AKR1C13) from mouse stomach."FEBS letters. 459 (3). 433-437 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki-Yamamoto, T., Nishizawa, M., Fukui, M., Okuda-Ashitaka, E., Nakajima, T., Ito, S., and Watanabe. K.: "cDNA cloning, expression, and characterization of human prostaglandin F synthase"FEBS letters. 462 (3). 335-340 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki-Yamamoto, T., Yokoi. H., Tsuruo, Y., Watanabe, K.and Ishimura, K.: "Identification of prostaglandin F-producing cells in the liver."Histochem. Cell Biol.. 112 (6). 451-456 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishizawa, M., Nakajima, T., Yasuda, K., Kanzaki, H., Sasaguri, Y., Watanabe, K., and Ito, S.: "Close Kinship of Human 20α-Hydroxysteroid Dehydrogenase Gene with Three Aldo-keto Reductase Genes."Genes Cells.. 5 (2). 111-125 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki-Yamamoto, T., Toida, K, Tsuruo, Y., Watanabe, K.and Ishimura, K.: "Immunocytochemical localization of lung-type prostaglandin F synthase in the rat spinal cord."Brain Res.. 877 (2). 391-395 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Dieter P, Scheibe R, Jakobsson PJ, Watanabe K, Kolada A, Kamionka S.: "Functional coupling of cyclooxygenase 1 and 2 to discrete prostanoid synthases in liver macrophages."Biochem Biophys Res Commun.. 276 (2). 488-492 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishizawa,M.,Watanabe,K.,and Ito,S.: "Close Kinship of Human 20a-Hydroxysteroid Dehydrogenase Gene with Three Aldo-keto Reductase Genes."Genes Cells.. 5・2. 111-125 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki-Yamamoto,Watanabe,K.and Ishimura,K: "Immunocytochemical Iocalization of lung-type prostaglandin F synthase in the rat spinal cord"Brain Res.. 877・2. 391-395 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Dieter P,Watanabe K,and Kamionka S.: "Functional coupling of cyclooxygenase 1 and 2 to discrete prostanoid synthases in liver macrophages."Biochem Biophys Res Commun.. 276・2. 488-492. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 渡部紀久子,山本(鈴木)登志子: "現代化学増刊38 プロスタグランジン研究の最前線PGE合成酵素およびPGF合成酵素"東京化学同人. 224(5) (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 渡部紀久子: "酵素ハンドブック"朝倉書店(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Watanabe,K.,Kurihara,K.,and Suzuki,T.: "Purification and Characterization of Membrance-bound Prostaglandin E Synthase from Bovine Heart."Biochim.Biophys.Acta. 1439. 406-414 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,K.,Fujii,Y.,and Watanabe,K.: "cDNA Cloning,Expression,and Mutagenesis Study of Liver-type Prostaglandin F Synthase."J.Biol.Chem.. 274. 241-248 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki-Yamamoto,T.,and Watanabe,K.: "cDNA cloning,expression,and characterization of human prostaglandin F synthase."FEBS letters. 426・3. 335-340 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki-Yamamoto,T.,Watanabe,K.and Ishimura,K.: "Identification of prostaglandin F-producing cells in the liver"Histochem.Cell biol.. 112・6. 451-456 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikeda,S.,Watanabe,K.,and Ito,S.: "Cloning and characterization of two novel aldo-keto reductases (AKR1C12 and AKR1C13)from mouse stomach"FEBS letters. 459・3. 433-437 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishizawa,M.,Watanabe,K.,and Ito,S.: "Close Kinship of Human 20a-Hydroxysteroid Dehydrogenase Gene with Three Aldo-keto Reductase Genes."Genes Cells.. 5・2. 111-125 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 伊藤誠二、近江谷克裕、八木達彦、渡部紀久子 共訳: "コア生化学"丸善. 288 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 渡部紀久子: "実験医学増刊 細胞膜・核内レセプターと脂溶性シグナル分子 プロスタグランジン(PG)D,E,F合成酵素,トロンボキサン合成酵素とPG,TX分解酵素"羊土社. 216(9) (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Suzuki, et.al.& K.Watanabe: "cDNA cloning, expression and mutagenesis study of liver-type prostaglandin F synthase" J.Biol.Chem. 274. 241-248 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Suzuki, ..., K.Watanabe & O Hayaishi: "Induction of hematopoietic prostaglandin D synthase in human megakaryocytic cells by phorbol ester" Biochem.Biophys.Res.Commun.241. 288-293 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Watanabe, et.al.: "Two types of microsomal prostaglandin E synthase : glutathione-dependent and -independent prostaglandin E synthases" Biochem.Biophys.Res.Commun.235. 148-152 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe Kikuko: "Two types of microsomal prostaglandin E synthase-Glutathione-dependent and-independent prostaglandin E synthases・・・" Biochem.Biophys.Res.Commun.235. 148-152 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki Toshiko: "Induction of hematopoietic prostaglandin D synthase in human megakaryocytic cells by phorbol ester" Biochem.Biophys.Res.Commun.241. 288-293 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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