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Ca^<2+> imaging of the slug nervous system underlying the odor-taste associative learning

Research Project

Project/Area Number 09680646
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

KAWAHARA Shigenori  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Assistant Professor, 大学院・薬学系研究科, 助手 (10204752)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsSlug / Invertebrate / Learning / Synchronization / Potential Oscillation / Ca^<2+>-sensitive dye / Optical Recording / カルシウム
Research Abstract

The procerebrum, a central olfactory organ of The terrestrial mollusc Limax marginatus, is clearly compartmentalized to a cell mass consisting of cell bodies of the local interneurons and neuropils where neurites of intrinsic and extrinsic neurons are interconnected. To clarify the mechanisms of odor information processing and odor-taste associative learning, responses of The procerebrum to olfactory input should be analyzed using an optical recording technique. For this purpose, it is most desirable to obtain signals only from the cell mass in its posterior view without interference by the signals from the underlying neuropils which contain those of neurites of extrinsic neurons as well. In the present study, fluorescent Ca^<2+> indicator dyes were used to stain selectively The cell mass for Their incorporation into cytoplasm of the cell soma, while potential-sensitive dyes were incorporated into the cell membranes to record predominantly electroactivities of the neuropils. A differential imaging system, which has a wide dynamic range virtually, well extracted signals of intracellular calcium from large background fluorescence which in general accompanied tissue-staining with acetoxymethyl esters of Ca^<2+> indicator dyes. Ca^<2+> signals conveying information on the neural activity of the CM exhibited synchronous oscillation, wave propagation, and responses to the excitation of the olfactory nerve. The present study indicates that optical recording with Ca^<2+> indicator dyes provides a useful means to selectively observe neural activities of The cell mass of the procerebrum.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] Tomita,A: "Removal after addition of NO-generating agents and 8-bromo-cyclic GMP causes morphological change of cultured cerebellar astrocytes : a new mode of NO action." Brain Research. 744. 344-346 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawahara,S.: "Comparative study on neural oscillation in the procerebrum of the terrestrial slugs lncilaria bilineata and Limax marginatus." Journal of Experimental Biology. 200. 1851-1861 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] de Barry,J.: "Time-resolved imaging of protein kinase C activation during sea urchin egg tertilization." Experimental Cell Research.234. 115-124 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima,H.: "Development of a fluorescent indicator for the bioimaging of nitric oxide." Biological and Pharmceutical Bulletin. 20. 1229-1232 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kishimoto,Y.: "Conditioned eyeblink response is impaired in mutant mice lacking NMDA receptor subunit NR2A." NeuroReport. 8. 3717-3721 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe,S.: "Morphological Characterization of the bursting and nonbursting neurons in the olfactory centre of the terrstrial slug Limax marginatus." Journal of Experimental Biology. 201. 925-930 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura,T.: "Optical recording analysis of olfactory response of the PC lobe in the slug brain." Learning and Memory. 4. 389-400 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima,H.: "Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore." Chemical and Pharmceutical Bulletin. 46. 373-375 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima,H.: "Detection and imaging of nitric oxide with novel fluorescent indicators : Diaminofluoresceins." Analytical Chemistry. 70. 2446-2453 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakagawa-Inoue,A.: "Odorant-evoked increase in cytosolic free calcium in cultured antennal neurons of blowflies." Zoological Science. 15. 661-666 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inoue,T.: "Selective optical recording of the neural activity in the olfactory center of land slug using a calcium indicator dye." Bioimages. 6. 59-67 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe,S.: "Glutamate induces Cl^- and K^+ currents in the olfactory intemeurons of the terrestrial slug." Journal of Comparative Physiology A. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川原茂敬: "ナメクジ脳にリズムを探る" 実験医学. 15. 2234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川原茂敬: "神経細胞におけるカルシウムイメージング" 細胞. 30. 7-10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 渡辺 恵: "ナメクジの嗅覚-味覚連合学習" 放射線科学. 42. 26-34 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川原茂敬: "瞬目反射の古典的条件付けと小脳・海馬" 放射線科学. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomita, A.: "Removal after addition of NO-generating agents and 8-bromo-cyclic GMP causes morphological change of cultured cereballar astrocytes : a new mode of NO action." Brain.Res.744. 344-346 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawahara, S.: "Comparative study on neural oscillation in the procerebrum of the terrestrial slugs Incilaria bilineata and Limax marginatus." J.Exp.Biol.200. 1851-1861 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] de Barry, J.: "Time-resolved imaging of protein kinase C activation during seaurchin egg fertilization." Exp.Cell Res.234. 115-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima, H.: "Developement of a fluorescentindicator for the bioimaging of nitric oxide." Biol.Pharm.Bull.20. 1229-1232 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kishimoto, Y.: "Conditioned eyeblink response is impaired in mutant mice lacking NMDA receptor subunit NR2A." NeuroReport. 8. 3717-3721 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe, S.: "Morphological characterization of the bursting and nonbursting neurons in the olfactory center of the terrstrial slug Limax marginatus." J.Exp.Biol.201. 925-930 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura, T.: "Optical recording analysis of olfactory response of the PC lobe in the slug brain." Learning and Memory. 4. 389-400 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima, H.: "Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore." Chem.Pharm.Bull.46. 373-375 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima, H.: "Detection and imaging of nitric oxide with novel fluorescent indicators : Diaminofluoresceins." Analytical Chemistry. 70. 2446-2453 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakagawa-Inoue, A.: "Odorant-evoked increase in cytosolic free calcium in cultured antennal neurons of blowflies." Zool.Sci.15. 661-666 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inoue, T.: "Selective optical recording of the neural activity in the olfactory center of land slug using a calcium indicator dye." Bioimages. 6. 59-67 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe, S.: "Glutamate induced Cl and K^+ currents in the olfactory intemeurons of the terrestrial slug." J.Comp.Physiol.A.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Inoue: "Selective optical recording of the neural activity in the olfactory center of land slug using a calcium indicator dye." Bioimages. 6. 59-67 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.,Nakagawa-Inoue: "Odorant-evoked increasein cytosolic free calcium in cultured antenal neurons of blowflies." Zoological Sci ence. 15. 661-666 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.,Kimura: "Optical recording analysis of olfactory response of the PC lobe in the slug brain." Learning and Memory. 4. 389-400 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.,Watanabe: "Morphological characterization of the bursting and nonbursting neurons in the olfactory center of the terrestrial slug Limax marginatus." Journal of Experimental Biology. 201. 925-930 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.,Kojima: "Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore." Chemical and Pharmceutical Bulletin. 46. 373-375 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.,Kojima: "Detection and imaging of nitric oxide with novel fluorescent indicators:Diaminofluoresceins." Analytical Chemistry. 70. 2446-2453 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.,Watanabe: "Glutamate induces Cl^- and K^+ currents in the olfactory interneurons of the terrestrial slug." Journal of Comparative Physiology A. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 川原茂敬: "神経細胞におけるカルシウムイメージング" 細胞. 30. 7-10 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 渡辺 恵: "ナメクジの嗅覚-味覚連合学習" 放射線科学. 42. 26-34 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 川原茂敬: "瞬目反射の古典的条件付けと小脳・海馬" 放射線科学. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 川原茂敬: "神経細胞におけるカルシウムイメージング" 細胞. 30(1). 7-10 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shigenori Kawahara: "Comparative study on neural oscillation in the procerebrum of the terrestrial slugs Incielaria bilineata and Limax marginatus." J.Experimental Biology. 200. 1851-1861 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.de Barry: "Time-resolved imaging of protein kinase C activaion during sea urchin egg fertilization" Experimental Cell Research. 234. 115-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoshi Watanabe: "Morphological characterization of the bursting and nonbursting neurons in the olfactory center of the terrestrial Slug Limax marginatus" J.Experimental Biology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Tetsuya Kimura: "Optical recording analysis of olfactory response of the PC lobe in the slug brain." Learning and Memory. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasushi Kishimoto: "Conditioned eyeblink response is impaired in mutant mice lacking NMDA receptor subunit NR2A" Neuro Report. 8. 3717-3721 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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