| Project/Area Number |
09680648
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Yokohama National University |
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Principal Investigator |
KATAHIRA Masato Yokohama National University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (70211844)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | DNA / RNA / NMR / quadruplex / duplex / telomere / protein / antitumor drug / 金属イオン / ドラッグ / リボザイム |
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| Research Abstract |
The novel quadruplex and duplex structures of d(GGAGGA), formed in the presence and absence of potassium ions, respectively, were determined by NMR.It is notable that the duplex structure is composed exclusively of non-standard G : G and A : A basepairs.
It was found for the first time that the RNA-binding protein, hnRNP D0 has the ability to transform the quadruplex structure to a single-stranded form. The structures and interactions with RNA of hnRNP DO and the related protein, Musashi1, were determined by NMR.It was suggested that this unique activity may be related to the maintenance of the telomere.
The structure of the novel antitumor drug, UCH9, complexed with DNA was determined by NMR.The B-form to A-form structural transition caused by drug-binding was identified, and its biological significance was implied.
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