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Regulatory mechanism of gene expression by Escherichio coli nucleoid protein H-NS

Research Project

Project/Area Number 09680671
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionNagoya University

Principal Investigator

UEGUCHI Chiharu  School of Agriculture, Nagaya University Assistant Professor, 農学部, 助手 (20232738)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsH-NS / gene regulation / global repressor / nucleoid protein / gene silencing / DNA-binding protein / LeuO / シグマ因子 / ストレス応答
Research Abstract

The Escherichia coil H-NS protein is one of the major constituents of the nucleoid structure. This protein has been implicated not only in the compact organization of the nucleoid structure, but also in the global regulation of gene expression. In this study, on systematic mutational analysis of hns, three distinct functional domains were found in H-NS, which appear to be responsible for DNA-binding, transcriptional repression and dimerization, respectively. Mutations in the C-terminal domain resulted in a loss of its DNA-binding ability, suggesting that this domain is directly involved in its binding to DNA.The N-terminal domain was suggested to be involved in the ability to repress transcription. The relatively central portion of H-NS was found to be involved in protein-protein interaction, especially dimerization. The functional significance for each domain was also clarified. In addition, expression of the bgl operon was found to be affected by only a subset of hns mutations in a highly allele-specific manner. This finding is also addressed with regard to a unique regulatory mechanism (i.e. silencing) for the bgl operon, which is partly mediated by H-NS.
The Escherichia coli bgl operon is of interest since its expression is silent (phenotypically Bgl^-), at least under standard laboratory conditions. To identify a trans-acting factor(s) that is presumably relevant to the regulation of bgl, I screened mutants exhibiting Bgl+ phenotype. By a random insertion mutagenesis with mini-Tn10, a novel type of mutation was obtained. In this case, the insertion mutation was found to be just in front of the leuO gene encoding a putative LysR-like DNA-binding protein. Genetic analyses revealed that an overproduction of LeuO in the wild-type cells causes the phenotype of Bgl^+.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Ueguchi C.,Suzuki T.,Yoshida T.,Tanaka K.,Mizuno T.: "Systematic mutational analysis revealing the functional domain organization of Escherichia coli nucleoid protein H-NS." J.Mol.Biol.263. 149-162 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tabata K.,Kashiwagi S.,Mori H.,Ueguchi C.& Mizuno T.: "Cloning of a cDNA encoding a putative metal-transporting P-type ATPase from Arabidopsis thaliana." Biochim.Biophys.Acta. 1326. 1-6 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueguchi C.,Seto C.,Suzuki T.,Mizuno T.: "Clarification of the dimerization domain and its functional significance for the Escherichia coli nucleoid protein H-NS." J.Mol.Biol.274. 145-151 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueguchi C.,Ohta T.,Seto C.,Suzuki T.,Mizuno T.: "The leuO gene-product has a latent ability to relieve the bgl silencing in Escherichia coli." J.Bacteriol.180. 190-193 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Imamura A.,Hamaki N.,Umeda H.,Nakamura A.,Suzuki T.,Ueguchi C.,Mizuno T.: "Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis." Proc.Natl.Acad.Sci.USA. 95. 2691-2696 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamashino T.,Isomura M.,Ueguchi C.,Mizuno T.: "The yhhP gene encoding a small ubiquitous protein that is essential for the normal cell-growth of Escherichia coli." J.Bacteriol.180. 2257-2261 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taniguchi M.,Kiba T.,Sakakibara H.,Ueguchi C.,Mizuno T.,Sugiyama T.: "Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate." FEBS Lett.429. 259-262 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamada H.,Hamaki N.,Imamura A.,Ueguchi C.,Mizuno T.: "An Arabidopsis protein that interacts with the cytokinin-inducible response regulator,ARR4,implicated in the His-Asp phosphorylay signal transduction." FEBS Lett.436. 76-80 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki T.,Imamura A.,Ueguchi C.,Mizuno T.: "Histidine-containing phosphotransfer(HPt)signal transducers implicated in His-to-Asp phosphotransfer signaling in" Plant Cell Physiol.39. 1258-1268 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuno T.&Ueguchi C.: "Molecular chaperone and protein-folding saguences" University Press, (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueguchi C., Suzuki T., Yoshida T., Tanaka K.and Mizuno T.: "Systematic mutational analysis revealing the functional domain organization of Escherichia coli nucleoid protein H-NS." J.Mol.Biol.263. 149-162 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tabata K., Kashiwagi S., Mori H., Ueguchi C.and Mizuno T.: "Cloning of a cDNA encoding a putative metal-transporting P-type ATPase from Arabidopsis thaliana." Biochim.Biophys.Acta.1326. 1-6 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueguchi C., Seto C., Suzuki T.and Mizuno T.: "Clarification of the dimerization domain and its functional significance for the Escherichia coli nucleoid protein H-NS." J.Mol.Biol.274. 145-151 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueguchi C., Ohta T., Seto C., Suzuki T.and Mizuno T.: "The leuO gene-product has a latent ability to relieve the bg/silencing in Escherichia coli." J.Bacteriol.180. 190-193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Imamura A., Hanaki N., Umeda H., Nakamura A., Suzuki T., Ueguchi C.and Mizuno T.: "Response regulators implicated in His-to-Asp Phosphotransfer signaling in Arabidopsis." Proc.Natl.Acad.Sci.USA. 95. 2691-2696 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamashino T., Isomura M., Ueguchi C.and Mizuno T.: "The yhhP gene encoding a small ubiquitous protein that is essential for the normal cell-growth of Escherichia coli." J.Bacteriol.180. 2257-2261 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taniguchi M., Kiba T., Sakakibara H., Ueguchi C., Mizuno T.and Sugiyama T.: "Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate." FEBS Lett.429. 259-262 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamada H., Hanaki N., Imamura A., Ueguchi C.and Mizuno T.: "An Arabidopsis protein that interacts with the cytokinin-inducible response regulator, ARR4, implicated in the His-Asp phosphorylay signal transduction." FEBS Lett.436. 76-80 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki T., Imamura A., Ueguchi C.and Mizuno T.: "Histidine-containing phosphotransfer (HPt) signal transducers implicated in His-to-Asp phosphotransfer signaling in Arabidopsis." Plant Cell Physiol.39. 1258-1268 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuno T.and Ueguchi C.: CbpA in Molecular chaperone and Protein-folding sequences. ed.Gething M.J.University Press, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueguchi, Ohta, Seto, Suzuki & Mizuno: "The leuO gene-product has a latent ability to relieve the bgl silencing in Escherichia coli." J.Bacteriol.180. 190-193 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Imamura, Hanaki, Umeda Nakamura, Suzuki, Ueguchi & Mizuno: "Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis." Proc.Natl.Acad.Sci.USA. 95. 2691-2696 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamashiro, Isomura, Ueguchi & Mizuno: "The yhhP gene encoding a small ubiquitous protein that is essential for the normal cell-growth of Escherichia coli." J.Bacteriol.180. 2257-2261 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Taniguchi, Kiba, Sakakibara, Ueguchi, Mizuno & Sugiyama: "Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate." FEBS Lett.429. 259-262 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamada, Haraki, Imamura, Ueguchi & Mizuno: "An Arabidopsis protein that interacts with the cytokinin-inducible response regulator,ARR4,implicated in the His-Asp phosphorylay signal transduction." FEBS Lett.436. 76-80 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki, Imamura, Ueguchi & Mizuno: "Histidine-containing phosphotransfer(HPt)signal transducers implicated in His-to-Asp phosphotransfer signaling in Arabidopsis." Plant cell Physiol.39. 1258-1268 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tabata T., Kashiwagi S., Mori. H., Ueguchi C & Mizuno T.: "Cloning of a cDNA encoding a putative metal-transporting P-type ATPase from Arabidopsis thaliana." Biochim. Biophys. Acta. 1326. 1-6 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ueguchi C., Seto C., Suzuki T. & Mizuno T.: "Clarification of the dimerization domain and its functional significance for the Escherichia coli nucleoid protein H-NS." J.Mol.Biol.274. 145-151 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ueguchi C., Ohta T., Seto C., Suzuki T. & Mizuno T.: "The leuO gene-product has a latent ability to relieve the bgl silencing in Escherichia coli" J.Bacteriol.180. 190-193 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Inamura A., Hanaki N., Uweda H Nakamura, Suzuki, Ueguchi & Mizuno: "Occurrence of a group of response regulators implicated in the His-Asp phosphotransfer signaling in Arabidopsis." Proc.Natl.Acad.Sci.USA. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mizuro T & Ueguchi C: "Molecular Chaperone and Protein-Folding Sequences(Ed.Gething, M-J.)" University Press, (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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