| Project/Area Number |
09680684
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Institute of Development, Aging and Cancer, Tohoku University |
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Principal Investigator |
WATANABE Toshio Institute of Development, Aging and Cancer, Tohoku University, Associate Professor, 加齢医学研究所, 助教授 (60201208)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | muscle development / cytoskeleton / stress fiber / PEBP2beta / acute myeloid leukemia / chimeric gene / immunohistochemistry / transcription factor |
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| Research Abstract |
The PEBP2beta gene encodes a non-DNA binding subunit of a heterodimeric transcription factor, PEBP2, in the chromosomal inversion 16 associated with human acute myeloid leukemia (AML), the PEBP2beta and smooth musclemysin heavy chain (SMMHC) genes fuse each other and the chimeric PEBP2beta-SMMHC gene is generated. We analyzed functions of cytoplasmic-located PEBP2beta and PEBP2beta-SMMHC proteins, which are distinct from their functios as a subunit of transcription factor. The results are as follows.
1)Differentiation dependent expression and distinct subcellular localization of the protooncogene product, PEBP2beta/CBFbeta, in muscle development.
2)The protooncogene product, PEBP2beta/CBFfbeta, is mainly located in the cytoplasm and has an affinity with cytoskeletalstructures.
3)The chimeric protein, PEBP2beta-SMMHC, disorganizes cytoplasmic stress fibers and inhibits transcriptionalactivation.
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