REGULATION OF CELL GROWTH AND DIFFERENTIATION BY A HOMEOTIC GENE AND CELL CYCLE CONTROLLING GENES
| Project/Area Number |
09680699
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | KOCHI MEDICAL SCHOOL |
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Principal Investigator |
TOMINAGA Akira KOCHI MEDICAL SCHOOL,MEDICAL BIOLOGY,PROFESSOR, 医学部, 教授 (50172193)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yoshiya KOCHI MEDICAL SCHOOL,MEDICAL BIOLOGY,RESEARCH ASSISTANT, 医学部, 教務職員 (60243846)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | HOMEOTIC GENE / BLOOD CELLS / NEURAL CELLS / DIFFERENTIATION / PROLIFERATION / ONCOGENE / PU.1 / CYCLIN D1 |
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| Research Abstract |
Goosecoid gene (gsc) is expressed in the dorsal lip area of Xenopus embryo and functions as a Spemann's organizer. It has a dorsalizing effect in Xenopus embryo. Expression of gsc in erythroblastcell line F5-5 (F5-5-gsc74-3) suppressed the hemoglobin production in response to activin. A species of 38-40 kDa proteins associated with retinoblastoma protein (Rb) was pulled down with GOOSECOID beads. Both GOOSECOID and Rb bind to N-terminal portion of PU.1. This suggests that GOOSECOID competes with Rb for its binding to PU.1. Forced expression of PU.1 in F5-5-gsc74-3 rescued the ability of differentiation to produce hemoglobin in response to activin. On the other hand, expression of gsc in rat pheochromocytoma cell line PC12 enhanced the neurite outgrowth in response to NGF.Expression of neurofilament-L and MAPlb were enhanced even without NGF.Although expression of gsc did not have any effects on the S phase period of erythroid cells, its expression diminished the S phase period in PCI2 cells. There were no substantial changes at the expression level of cyclin Dl either in the presence or absence of NGF.
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Report
(3 results)
Research Products
(6 results)
