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REGULATORY MECHANISMS OF DORSO-VENTRAL AXIS FORMATION IN AMPHIBIAN EMBRYO.

Research Project

Project/Area Number 09680721
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

MAENO Mitsugu  NIIGATA UNIVERSITY, FACULTY OF SCIENCE, ASSOCIATE PROFESSOR, 理学部, 助教授 (10190315)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsXenopus laevis / homeobox / dorso-ventral axis / organizer / BMP-4 / dominant-negative / neural induction / FGF / goosecoid / 腹側化 / 脊索 / 筋肉 / msx-1 / Xwnt-8 / 赤血球
Research Abstract

I have investigated several cDNA clones, which encode for ventralizing agents. Among these factors, the roles of msx-1 and msx-2 in the dorsoventral axis formation have been extensively analyzed. These transcriptional repressor proteins were expressed in presumptive ectoderm and mesoderm ventrally, but not dorsally, at gastrula stage. Both msx-1 and msx-2 possessed a similar ventralizing activity, but that of msx-2 was higher than of msx-1, as revealed by the injection of RNA into dorsal blastomeres. The secondary axis induced by the injection of tBR was rescued by simultaneous injection of msx-1/msx-2. Furthermore, if VP-16/msx-1 fusion RNA, which was expected to function as a dominant negative msx-1, was injected into the ventral blastomeres, partial Secondary axis was formed. Therefore, msx-1 and msx-2 transduce a main tract of BMP-4 signaling. Msx factors also have a role in neural tissue induction in ectodermal cells. If msx-1 was coinjected with neural inducer genes such as noggin or cerberus, the expression of N-CAM was completely suppressed. As also shown in tBR (which directly induces neural tissue in animal cap), VP 16/msx-1 fusion RNA was able to induce neural tissue directly in animal cap cells. The results indicate that msx proteins play a central role in establishing dorso-ventral axis and in neural tissue formation in gastrulating embryo, by antagonizing the expression of organizer genes. The expression and function of GATA-1 (another transcriptional regulator) and BMP-1 (metalloprotease) in Xenopus embryo have been also investigated in this study.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Lin 他6名: "XBMP-1B(Xtld), a Xenopus homolog of dorsal-ventral polarity gene in Drosophila, modifies tissue phenotypes of ventral explants"Develop. Growth & Differ.. 39・1. 43-51 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda 他5名: "Xmsx-1 modifies mesodermal tissue pattern along dorsoventral axis in Xenopus laevis embryo"Development. 124・13. 2552-2560 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shibata 他3名: "GATA-1 inhibits the migration of dorsal mesodern during gastrulation of notochord in Xenopus embryo"Biochem. Biophy. Res. Commun.. 252・1. 241-248 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishimura 他4名: "Immunodetection of Xenopus bone morphogenetic protein-1 in adult and embryonic cells"Growth Factor. 16・3. 171-177 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeda 他5名: "Xenopus msx-1 regulates the dorso-ventral axis formation by Xwnt-8-mediated pathway"Comp. Biochem. Physiol.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishimura 他5名: "Involvement of BMP-4/msx-1 and FGF pathways in neural induction in Xenopus embryo"Develop. Growth Differ.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Lin, J. J., Maeda, R., Ong, R., Kim, J., Lee, L. M., Kung, H. F., and Maeno, M.: "XBMP-1B (Xtld), a Xenopus homolog of dorso-ventral polarity gene in Drosophila, modifies tissue phenotypes of the ventral explants."Develop. Growth Differ.. 39. 43-51 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda, R., Kobayashi, A., Sekine, R. Lin, J. J., Kung, H. F., and Maeno, M.: "Xmsx-1 modifies mesodermal tissue pattern along dorsoventral axis in Xenopus laevis embryo."Development. 124. 2552-2560 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shibata, K., Ishimura, A., and Maeno, M.: "GATA-1 inhibits the migration of dorsal mesoderm during gastrulation and the formation of notochord in Xenopus embryo."Biochem. Biophy. Res. Commun.. 252. 241-248 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishimura, A., Princler, G. L., Lin, J. J., Kung, H. F., and Maeno, M.: "Immunodetection of Xenopus bone morphogenetic protein-1 in adult and embryonic cells."Growth factor. 16. 171-177 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeda, M., Saito, Y., Sekine, R., Onizuka, I., Maeda R., and Maeno, M.: "Xenopus msx-1 regulates the dorso-ventral axis formation by Xwnt-8-mediated pathway."Comp. Biochem. Physiol.. (In press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishimura, A., Maeda, M., Takeda, M., Kikkawa, M., Daar, I. O. and Maeno, M.: "Involvement of BMP-4/msx-1 and FGF pathways in neural induction in Xenopus embryo."Develop. Growth & Differ.. In press..

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Lin他6名: "XBMP-1B(Xtld), a Xenopus homolog of dorsal-ventral polarity gene in Drosophila, modifies tissue phenotypes of ventral explants."Develop. Growth & Differ.. 39・1. 43-51 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Maeda他5名: "Xmsx-1 modifies mesodermal tissue pattern along dorso-ventral axis in Xenopus laevis embryo."Development. 124・13. 2552-2560 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shibata他3名: "GATA-1 inhibits the migration of dorsal mesoderm during gastrulation of notochord in Xenopus embryo."Biochem. Biophy. Res. Commun.. 252・1. 241-248 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishimura他4名: "Immunodetection of Xenopus bone morphogenetic protein-1 in adult and embryonic cells."Growth Factor. 16・3. 171-177 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takeda他5名: "Xenopus msx-1 regulates the dorso-ventral axis formation by Xwnt-8-mediated pathway."Comp. Biochem. Physiol.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishimura他5名: "Involvement of BMP-4/msx-1 and FGF pathways in neural induction in Xenopus embryo."Develop. Growth Differ.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Lin 他6名: "XBMP-1B(Xtld),a Xenopus homolog of dorso-ventral poloritg gene in Drosophila,modifies tissuo Phenotypes of ventral explants." Develop.Growth Differ.39・1. 43-51 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maeda 他5名: "Xmsx-1 nodifies mecodermal tissue pattern along dorsoventral axis in Xenopus laevis embryo" Development. 124・13. 2552-2560 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shibata 他2名: "GATA-1 inhibits the migration of clorsal mesoderm during gastrulation and the formation of notochord in Xenopus embryo." Biochem.Biophys.Res.Commun.252・1. 241-248 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishimura 他4名: "Immurodetectron of Xenopus bone morphogenetic protein-1 in adult and embryonic cells." Growth Factor. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maeda,R.et.al.: "Xmsx-1 modifies mesodermal tissue pattern along……" Develpment. 124,13. 2553-2560 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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