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Gene analysis of pectral finless mutant (pl) of Japanese Medaka(Oryzias latipes)

Research Project

Project/Area Number 09680723
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNagoya University

Principal Investigator

YODA Kinnya  Nagoya University Bioscience Center, Assistant, 生物分子応答研究センター, 助手 (30126916)

Co-Investigator(Kenkyū-buntansha) 植村 和彦  国立科学博物館, 地学研究部, 研究員 (50000138)
鈴木 三男  東北大学, 大学院・理学研究科, 教授 (80111483)
高田 克彦  九州大学, 農学部, 助手 (50264099)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsOryzias latipes / pectral finless mutant (pl) / left pectral finless mutant (lpl) / backcross / gemetic linkage map / RAPD method / 球果植物綱 / 染色体他* / 葉緑体DNA / RAPD法 / DNA塩基配列 / 核DNA / RAPD / 戻し交配 / 染色体地図 / ニホンメダカ / 形態形成 / 胸ヒレ欠失変異pl / 右胸ヒレ欠失変異lpl
Research Abstract

(a)backcross HNI/pl (female) x lp/pl (male): F1 offsprings (597) were obtained from 3 sets of backcross pair. Among them wildtype were 278 (46.6 %), pl=317 (53.1 %) and lpl=2 (0.3 %). We checked 500 offsprings more and found that frequency of appearance of lpl was about the same. The frequency of lpl appearance seems to be high.
(b) lpl-l (male) x lpl-2 (female): pl=90 (53 %), lpl=43 (25 %), wild type=38 (22 %), We observed early developmental stage of each embryos and found that approximately half (47) of the pl formed fin bud at early embryo stage and they disappeared after then.
(c) lpl-1 x pl/pl: The ratio of pl to lpl was not equal but most of the offsprings were pl and approximately 10 % were lpl. From these results we speculated that expression of lpl mutant might not be regulated by single gene but more than two genes take part in this event.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kinya Yoda et al.: "Vitro assembly of the CENP-B/α-SatelliteDNA/core histone complex : CENP-B caused nucleosome positioning"Genes to Cells. 3. 533-548 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinya Yoda et al.: "Centromere specific nucleosome structures in Human cells : Study of CENPA, the centromere specific histone H3"Gordon Research Conference on Molecular genetics. 1998・7月19日-23. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Okazaki,K.Yoda et al.: "Human Centromere : Specific chromatin organization and the alphoid array competent to produce functional centromere/kinetochore structure"Third APOCB congress Abstracts. 8月24-28日. 18-18 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinya Yada et al..: "Invitro assembly of the CENRB/α-satellite DNA/core histone complex: CENP-β causes nucleosome positioning"Genes to cells. 3. 533-548 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinya Yada et. Al.: "Gentromere specific nucleosome structures in Human Cells: Study of CENRA, the centromere specific histone H3"Gordon Research Conference on Molecular genetics. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Okazaki, K.Yoda et al..: "Human Centromere: Specific chromatin organization and the alphoid array competent to produce functional centromere / kinetochore structure"Third APOCB Congress Abstracts. 18-18 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinya Yoda at al.: "Invitro Assembly of the CENPB/α-satelite DNA/Core histone complex : CENP-B caeeses nucleosome Posetioning" Genes to cells. 3. 533-548 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kinya Yoda et al.: "Centromene Specific Nucleosome struetures in Human cells study of CENPA,the contromere specific histone H3" Gordon Research conference on Moleculor Genetics. 1998 7月19-23日. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kinya Yoda et al: "Site-specific base deletions in human α-satellite monomer DNA are asseciated unth regularly distrikated CENP-B boses" Chromosome Research. 5. 207-211 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kinya Yoda: "Centromere specific nucleosome structures in human cell" Keystone Symposium on Functional Organization of the Nucleus. 40-40 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 依田欣哉: "セントロメア特異的ヌクレオソーム構造(ヒトセントロメア特異的ビストcH3=CENP-Aの解析)" 第30回日本細胞生物学会大会抄録. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 依田欣哉: "ヒトセントロメア領域特異的ヌクレオソーム構造が形成する高次構造体の解析" 第20回日本分子生物学会年会抄録. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 安藤覚, 依田欣哉: "ヒトCENP-Aに対するモノクローナル抗体の作製" 第20回日本分子生物学年会抄録. (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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