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Development of A New Method to Investigate Cell Migration in the Developing Central Nervous System

Research Project

Project/Area Number 09680740
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionDepartment of Anatomy, Fukui Medical University

Principal Investigator

TAMAMAKI Nobuaki  Fukui Medical University, Dept.of Anatomy, Associate Professor, 医学部, 助教授 (20155253)

Co-Investigator(Kenkyū-buntansha) FUJIMORI Kazuhiro  Fukui Medical University, Dept.of Anatomy, Reserch Associate, 医学部, 助手 (60273025)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsMigration / Migration speed / Olfactory Buld / Thymin-dimer / UV-light / Fiber Optic Cable / チミンダイマー / 紫外線照射 / 色素性乾皮症 / ミュータントマウス / 光ファイバー
Research Abstract

We have devised a novel method to study cell migration in vitro and in vivo. The new method involves UV-irradiating cells through a fiber optic cable and subsequently identifying irradiated cells by virtue of the formation of thymine-dimer in the nuclei. We applied the new method to investigate migration of neuronal precursor cells in the rostral migratory stream in the neonatal rat olfactory bulb. In vitro, UV-irradiation for one second through the fiber optic cable allowed formation of enough thymine-dimers to be detected immunohistochemically after 6 hours of incubation. A significant proportion of irradiated cells continued to migrate in the same direction at the same speed as before irradiation. There was no significant difference in the cell-migration distance at 6 hours with or without the UV-irradiation in vitro. We applied this UV-thymine-dimer labeling method to study cell migration in the olfactory bulb in vivo, With this method, we could find that a small number of cells migrated also caudally. Three times more cells in the SV of the olfactory bulb migrated rostrally, but there was also a significant extent of caudally directed cell migration. The new method also allowed us to measure the speed of cell-migration, which was estimated to be about 70 ? m/hr to the rostral direction and 37 ? m/hr to the caudal direction, at maximum.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Tamamaki N.et al.: "The development of afferent fiber lamination in the infrapyramidal Blade of the rat dentate gyrus." J.Comp.Neurol.in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamamaki N.et al.: "Tangential cell migration in the neocortex monitored by marking nuclei with UV-irradiation via a fiber optic cable." Development. submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuaki Tamamaki, Yoshihiko Sugimoto, Kiyoji Tanaka, and Rumiko Takauji: "Tangential cell migration in the neocortex monitored by marking nuclei with UV-irradiation via a fiber optic cable." Development. (submitted). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamamaki N.et al.: "The development of afferent fiber lamination in the infrapyramidal blade of the rat dentate gyrus." J.Comp.Neurol.in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Tamamaki,N.et al.: "Intermediate zone and intermediate zone neurons: Do IZ neurons pioneer the layer V neurons in rats?." Developmental Neuroscience. 19. 112-116 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamamaki,N.et al.: "Origin and route of tangentially migrating neurons in the developing neocorteical intermediate zone." Journal of Neuroscience. 17. 8313-8323 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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