| Project/Area Number |
09680748
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Sapporo Medical University School of Medicine |
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Principal Investigator |
NINOMIYA Takafumi Sapporo Medical University, School of Medicine, Lecturer, 医学部, 講師 (80156140)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Dorsal root ganglion / Neurite formation / Nerve growth factor / NGF receptor / Aging / Cell culture / 神経突起 / 偽単極細胞 / 神経栄養因子 / レセプター |
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| Research Abstract |
In aging dorsal root ganglion (DRG) cell culture, DRG cells display the multipolar, bipolar or pseudounipolar forms. Some pseudounipolar neurons had many thin neurites elongated from the junction of the stem process or a structure-like swelling body or glomerulus (unipolar 2 cell type). These structures were not seen in fetal or newborn mouse DRG cell cultures. Unipolar2 neurons appeared with an incidence of about 5% in a medium without NTF or supplemented with NT-3, NT-4 or BDNF.However unipolar2 neurons increased about 30% in a medium supplemented with NGF.There is no difference of the number of surviving neurons in each of culture medium. The appearance of this type cell was affected by NGF, not affect other neurotrophic factors. It is suggested that adult DRG neurons remained only the low-affinity NGF-receptor(p75) but not the high-affinity NGF receptors(trk A, B, or C).
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