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GANGLIOSIDE GT1B AND GQ1B AS THE MARKER OF SYNAPSE FORMATION IN P19EC CELLS

Research Project

Project/Area Number 09680756
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionTOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE

Principal Investigator

OSANAI Taka  TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE,BIOCHEMICAL CELL RESEARCH, SENIOR RESEARCHER, 生命情報研究部門, 研究員 (60126018)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Keywordsganglioside / P19 cell line / neuronal cells / sialyltransferases / synaptogenesis / growth cone / GAP-43 / synaptophysin / 多能性幹細胞 / レチノイン酸
Research Abstract

We have studied ganglioside alterations and their enzymatic basis during the course of neural differentiation of mouse embryonic carcinoma cell line P19. This cell line can differentiate into neurons and astrocytes on cell aggregation after treatment with retinoic acid (RA) or into muscle cells on dimethyl sulfoxide (DMSO) treatment. GD3, detected on immunostaining after thin-layer chromatography (TLC) with monoclonal antibody (MAb) R24, was markedly present in aggregates treated with RA.GM3 synthase (alpha2,3-sia1yltransferase, SAT-I) in neurons was found to exhibit the highest activity. GD3 synthase (alpha2,8-sialyltransferase, SAT-II) and GD3 synthase mRNA, as analyzed by Northern blotting, were also markedly present in aggregates and neurons induced by RA.However, on treatment with DMSO, which induces muscle cells, there was no change in the level of GD3 synthase activity, and its transcript was hardly detected during the course of muscle differentiation. GTlb synthase (alpha2,3-sialyltransferase, SAT-IV) was present at similar levels in undifferentiated cells and aggregates treated with RA, but a higher level was observed in neurons. On the other hand, the level of GQlb synthase (alpha2,8-sialyltransferase, SAT-V) in RA-induced aggregates was significantly higher than that in neurons. These results show that RA but not DMS0 induces the expression of GM3, GD3, GTlb and GQlb synthases, and particularly GD3 synthase mRNA, in the ganglioside biosynthetic pathway during the neural differentiation of embryonic carcinoma P19 cells.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Taka Osanai: "Retinoic acid induces the expression of GD3 synthase mRNA during the differentiation process in murine embryonal carcinoma cell line P19 cells" Chemistry and Physics of Lipids. 82・2. 146 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taka Osanai: "Glycolipid sialytransferases are enhanced during neural differentiation of mouse embryonic carcinoma cells,P19" Biochem.Biophys.Res.Commun.241・2. 327-333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小山内たか: "レチノイン酸による神経細胞分化誘導とGD3合成酵素遺伝子の発現" 神経化学. 36・3. 276 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小山内たか: "P19神経細胞分化におけるLewis xエピトープ糖脂質について" 神経化学. 37・3. 359 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taka Osanai: "Retinoic acid induces the expression of GD3 synthase mRNA during the differentiation process in murine embryonal carcinoma cell line P19 cells" Nuerochemical Research. 24・1. 175 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TAKA OSANAI: "RETINOIC ACID INDUCES THE EXPRESSION OF GD3 SYNTHASE MRNA DURING THE DIFFERENTIATION PROCESS IN MURINE EMBRYONAL CARCINOMA CELL LINE P19 CELLS" CHEMISTRY & PHYSICS OF LIPIDS. 82. 146 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TAKA OSANAI: "GLYCOLIPID SIALYLTRANSFERASES ARE ENHANCED DURING NEURAL DIFFERENTIATION OF MOUSE EMBRYONIC CARCINOMA CELLS,P19" BIOCHEM.BIOPHYS.RES.COMMUN.241. 327 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TAKA OSANAI: "RETINOIC ACID INDUCES THE EXPRESSION OF GD3 SYNTHASE MRNA DURING THE DIFFERENTIATION PROCESS IN MURINE EMBRYONAL CARCINOMA CELL LINE P19 CELLS" NUEROCHEMICAL RES.24. 175 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小山内たか: "P19神経細胞分化におけるLewis x エピトープ糖脂質について" 神経化学. 37・3. 359 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Taka Osanai: "Retinoic acid induces the expression of GD3 synthase mRNA during the differentiation process in murine embryonal carcinoma cell line P19 cells" Nuerochemical Research. 24・1. 175 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Taka Osanai: "Glycolipid sialyltransferases are enhanced during neural differentiation of mouse embryonic carcinoma cells,P19" Biochem.Biophys.Res.Commun.241・2. 327-333 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Taka Osanai: "Retinoic acid induces the expression of GD3 synthase mRNA during the differentiation process in murine embryonal carcinoma cell line P19 cells" Chemistry and Physics of Lipids. 82・2. 146 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小山内たか: "レチノイン酸による神経細胞分化誘導とGD3合成酵素遺伝子の発現" 神経化学. 36・3. 276 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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