• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

"MOLECULAR MODE OF ACTION OF NEUROTROPHIC FACTOR,PROSAPOSIN"

Research Project

Project/Area Number 09680764
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionEHIME UNIVERSITY

Principal Investigator

SANO Akira  EHIME UNIVERSITY, 医学部, 助教授 (30178800)

Co-Investigator(Kenkyū-buntansha) UENO Shu-ichi  EHIME UNIVERSITY, 医学部附属病院, 助手 (80232768)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPROSAPOSIN / NEUROTROPHIC / 18-MER PEPTIDE / DOPAMINERGIC / NEURON / ドーパミン / パーキンソン病 / ラット海馬神経細胞 / 母乳
Research Abstract

Prosaposin, the precursor of saposins which activate sphingolipid hydrolases, has been identified as a neurotrophic factor. The neurotrophic activity of prosaposin on human neuroblastoma cells, hippocampal neurons, and PC 12 cells has been shown to reside in the NH_2-terminal hydrophilic sequence of the C-domain of prosaposin. It has recently demonstrated that intracerebral transplantation of testis-derived sertoli cells promotes functional recovery in rats with 6-hydroxydopamine-induced hemiparkinsonism, and in vitro experiments demonstrated enhanced dopaminergic neuronal survival and outgrowth when embryonic neurons were cultured with medium in which rat Sertoli cells had been grown. Since prosaposin is one of the major Sertoli cell secretory proteins, its trophic effect on dopaminergic neurons is conceivable. Here we show that a peptide comprising the 18-amino-acid sequence (18-mer peptide ; LSELIINNATEELLIKGL) located in the NH_2-terminal hydrophilic sequence of the C-domain of rat prosaposin promoted survival and neurite outgrowth of tyrosine hydroxylase-positive neurons on embryonic day 15 rat ventral mesencephalic cultures.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Patton et al.: "Prosaposin a Neurotrophic Factor:Presence and Properties in Boune Milk" J.Pairy Sci.80. 264-272 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kushihata et al.: "Expression of basic fibroblast growth factor-like immunoreactiuty in the nuclei of regenerating hepafocytes." Cell.Tiss.Res.288. 517-527 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小谷泰教・佐野 輝: "プロサポシンの神経栄養因子作用" 医学のあゆみ 別冊 神経細胞死制御. 233-237 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 佐野 輝(分担): "領域別症候群シリーズNo19 先天性代謝異常症候群(下巻)" 日本臨床, (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Patton, G.S.Carson, M.Hiraiwa, J.S.O'Brien, and A.Sano: "Prosaposin a Neurotrophic Presence and Properties in Bovine Milk." J.Dairy Sci. 80. 264-272 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] F.Kushihata, S.Matsuda, A.Sano, J.Aburaya, Y.Shimahara, N.Kobayashi, and M.Sakanaka: "Expression of basic fibroblast growth factor-like immunoreactivity in the nuclei of regenerating" Cell Tiss.Res.288. 517-527 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Sano: "Saposin C deficiency." Ryoikibetus Shokogun Shirizu. 19 Pt 2. 240-2 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kotani and A.Sano: "Saposin B deficiency." Ryoikibetus Shokogun Shirizu. 19 Pt 2. 417-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kondoh and A.Sano: "Prosaposin deficiency." Ryoikibetus Shokogun Shirizu. 19 Pt 2. 414-6 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kondoh and A.Sano: "GM2 activator protein deficiency." Ryoikibetsu Shokogun Shirizu. 19 Pt 2. 411-3 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Patton et al.: "Prosaposin a Neurotrophic Foctor: Presence and Properties in Bovine Milk" J.Pairy Sci.80. 264-272 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kushihata et al.: "Expression of basic fibroblast grouth foctor-like immunoreactivity in the nuclei of regenerating hepatocytes." Cell.Tiss.Res.288. 517-527 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小谷泰教・佐野輝: "プロサポシンの神経栄養因子作用" 医学のあゆみ 別冊 神経細胞死制御. 233-237 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 佐野 輝(分担): "領域別症候群シリーズNo19 先天性代謝異常症候群(下巻)" 日本臨床, (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Patton et al.: "Prosaposin,a neurotroplic factor:presence and properties in milk" J.Dairy Sci. 80. 264-272 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 阪中雅広ら: "脳虚血モデルを用いた虚血脳保護因子の評価系" アニテックス. 9. 149-155 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 近藤啓次、佐野輝: "プロサポシン欠損症" 日本臨床. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 小谷泰教、佐野輝: "サポシンB欠損症" 日本臨床. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐野 輝: "サポシンC欠損症" 日本臨床. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 近藤啓次、佐野輝: "GM2活性化蛋白質欠損症(GM2ザンブリオシドーシスAB型)" 日本臨床. (印刷中).

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi