| Project/Area Number |
09680783
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH |
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Principal Investigator |
ITOH Kouichi TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH, THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, RESEARCHER, 東京都臨床医学総合研究所, 研究員 (30291149)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | NEURONAL ACTIVITY / NEURAL CELL ADHESION MOLECULES / NEUROTROPHIC FACTORS / trk / DRG NEURONS / CELL CULTURES / MOUSE / ELECTRICAL STIMULATION / Neuron / Plasticity / Cell Adhesion Molecules / LTP / Brain / Nervous System / plasticity / cell adhesion Molecule / Nervous system |
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| Research Abstract |
We studied the relationship between neuronal activity, neural cell adhesion molecules and neurotrophins in mouse dorsal root ganglion (DRG) neurons during development in vitro. The nervous system structure is modified by electrical activity in developing neural circuits, but the molecular mechanisms are not well under stood. Recognition molecules and neurotrophic factors regulate cell interactions during development. Therefore, the possible involvement of neural cell adhesion molecules (L1, NCAM and N-cadherin), and of neurotrophins (NGF, BDNF and NT-3) in activity-development was studied by delivering electrical pulses to mouse DRG neurons in cultures. After 5 days exposure to NGF, but not after exposure to BDNF, the NT-3 and L1 levels increased 3〜4 fold. Low frequency stimulation (0.1 Hz for 5 days) decreased L1 and trkC levels in the absence of NGF. Regulation of the expression of L1 and trkC by specific patterns of impulse activity may influence cell interactions coordinating structure and function of the nervous system at critical developmental stages.
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