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Analysis of neural plasticity by transcription factor Otf6

Research Project

Project/Area Number 09680792
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionThe Institution of Physical and Chemical Research (RIKEN) (1998)
Kyoto University (1997)

Principal Investigator

IKEDA Toshio  Riken, Behavioral Genetics, Scientist, 行動遺伝学技術開発チーム, 研究員 (80252526)

Co-Investigator(Kenkyū-buntansha) ITOHARA Shigeyoshi  Behavioral Genetics, Team Leader, 行動遺伝学技術開発チーム, チームリーダー(研究職) (60252524)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordstranscription factor / embryonic lethal / homologous recombination / knockout mouse / transgenic mouse / Otf6 / 細胞周期 / コンディショナルノックアウトマウス
Research Abstract

Mouse lacking the entire Otf6 (Oct-6, Scip, Tst-1) gene locus was generated by homologous recombination in embryonic stem cells. The heterozygous mutants developed normally and were fertile. No homozygous mutant offspring were obtained from intercrosses of heterozygotes, suggesting that this deletion mutation of Otf6 locus was essential for embryonic development. Homozygous mutant embryos died before 8 days post-coitus (dpc). The homozygous mutants were characterized by small size and morphological abnormality compared to the heterozygous mutants and wild types at 7.5 dpc. In addition the BrdU incorporation rate of embryo was reduced in homozygous mutants. In situ labeling for the fragmentation of the genome (TUNEL) suggested that apoptosis occurs in the embryonic cells of homozygous mutant. Consistent with the in vivo results, homozygous mutant manifested growth arrest and rapid death of the inner cell mass in vitro. It has been shown that Mdm2 and Brca1 deficient embryos arrested growth and died at similar embryonic stages. We found that expression of m dm2 but not Brca1 was nearly abolished in homozygous mutant embryos. These results suggest that the developmental arrest of the Otf6 deletion mutants could be caused by the m dm2 pathway.
To analyze the function of Otf6 in adult mouse brain, we produced the conditional gene targeting system using Cre-loxP system and are confirming the specificity of Cre recombinase expression in brain.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Minoguchi et al.: "Studies on the cell-type specific expression of RBP-L, a RBP-J family member, by replacement insertion of beta galaetosidase"J. Biochem( Tokyo). 126. 738-747 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itoh et al.: "Unaltered secretion of beta-amyloid precursor protein in gelatinase A (matrix metalloproteinase 2) deficient mice"J. Biol Chem. 272. 2389-2392 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Futatsugi et al.: "Facilitation of NMDA-independent LTP and apacial learning in mutant mice lacking Ryanodine receptor type 3"Neuron. 4. 701-173 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gomi et al.: "Learning induces a cdc-2 related protein kinase, KKIAMRE"J. Nueroscience. 19. 9530-9537 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakaguchi et al.: "Doc2a is an activity-dependent modulator of excitatory synaptic transmission"Eur. J. Neuroscience. 11. 4262-4268 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuwahara et al.: "Prion protein prevents neuronal cell death"Nature. 400. 225-226 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hoq et al.: "Insufficient resistance of Trehalose-6, 6'-Dimycolate-treated T-cell receptor delta gene mutant(TCRδ-/-)mice against influenza virus infection"Microbiol. Immunol. 43. 491-493 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takano et al.: "Protective roles of gamma delta T cells and interleukin-15 in Escherichia coli infection in mice"Infect. Immun.. 66. 3270-3278 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katohet al.: "Dynamic characteristics and adaptability of mouse vestibulo-ocular and optokinetic response eye movements and the role of the flocculo-olivary system revealed by chemical lesions"Proc. natl. Acad. Sci. USA. 95. 7705-7710 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itoh et al.: "Reduced Angiogenesis and Tumor Progression in Gelatinase A Deficient Mice"Cancer Res.. 58. 1048-1051 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Minoguchi, S., Ikeda T., Itohara, S., Kaneko, T., Okaichi, H., Honjo. T.: "Studies on the cell-type specific expression of RBP-L a RBP-J family member by replacement insertion of beta-galactosidase"J. Biochem. (Tokyo). 126. 738-747 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itoh, T., Ikeda T., Gomi, H., Nakao, S., Suzuki, T., Itohara, S.: "Unaltered secretion of beta-amyloid precursor protein in gelatinase A (matrix metalloproteinase 2)-deficient mice"J. Biol Chem.. 272. 22389-22392 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Futatsugi A., Kato K., Ogura H., Li S.-T., Nagata E., Kuwajima G., Tanaka K., Itohara S., and Mikoshiba K.: "Facilitation of NMDA-independent LTP and special learning in mutant mice lacking Ryanodine receptor type 3"Neuron. 4. 701-173 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gomi H., Sun W., Finch C. E., Itohara S., Yoshimi K., Thompson R. F.: "Learning induces a cdc-2 related protein kinase, KKIAMRE"J. Nueroscience. 19. 9530-9537 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakaguchi G. Manabe T., Kobayashi, K., Orita S Sasaki T Naito A Maeda M, Igarashi, H., Katsuura. G., Nishioka. H., Mizoguchi, A., Itohara S., Takahashi, t., and Takai, Y.: "Doc2a is an activity-dependent modulator of excitatory synaptic transmission"Eur. J. Neuroscience. 11. 4262-4268 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuwahara C., Takeuchi A. M., Nishimura T., Haraguchi K., Kubosaki A., Matsumoto Y., Saeki K., Matsumoto Y., Yokoyama T., Itohara S., Onodera T.: "Prion protein prevents neuronal cell death"Nature. 400. 225-226 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hoq M.M., Suzutani T., Nakaya K., Yoshida I., Ogasawara M., Takeda Y., Shibaki T., Itohara S., Yamamoto H., and Azuma M.: "Insufficient resistance of Trehalose-6,6'-Dimycolate-treated T-cell receptor delta gene mutant (TCRd-/-) mice against influenza virus infection"Microbiol. Immunol.. 43. 491-493 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takano M, Nishimura H, Kimura Y, Mokuno Y, Washizu J, Itohara S, Nimura Y, Yoshikai Y.: "Protective roles of gamma delta T cells and interleukin-15 in Escherichia coli infection in mice"Infect. Immun.. 66. 3270-3278 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katoh, A., Kitazawa, H., Itohara S., and Nagao, S.: "Dynamic characteristics and adaptability of mouse vestibulo-ocular and optokinetic response eye movements and the role of the flocculo-olivary system revealed by chemical lesions."Proc. natl. Acad. Sci. USA. 95. 7705-7710 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itoh, T., Tanioka, M., Yoshida, H., Yoshioka, T., Nishimoto, N. and Itohara S.: "Reduced Angiogenesis and Tumor Progression in Gelatinase A Deficient Mice"Cancer Res.. 58. 1048-1051 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 加藤 明 他: "Dynamic characterics and adaptability of mouse vestibula-ocular and optokinetic response eye movements and the role of the floaculo-olivarysystem reveales by chemical lesions" Proc.Natl.Acad.Sci.U.S.A.95・13. 7705-7710 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 伊藤 剛 他: "Reduced angiogenests and tumor progression in gelatinaseA deficient mice." Cancer Res.58・5. 1048-1051 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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