| Project/Area Number |
09680795
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Fukui Medical School |
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Principal Investigator |
HIGUCHI Takashi Fukui Medical University, Physiology, Professor, 医学部, 教授 (70106326)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Takuya Fukui Medical University, Physiology, Reseach Associate, 医学部, 助手 (70281186)
NARITA Kazumi Fukui Medical University, Physiology, Reseach Associate, 医学部, 助手 (80270958)
HONDA Kazumasa Fukui Medical University, Physiology, Associate Professor, 医学部, 助教授 (50143946)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | parturition / maternal behavior / milk-ejection reflex / hypothalamus / pituitary gland / nitric oxide synthase / paraventricular nucleus / supraoptic nucleus / 一酸化窒素 / 子宮 / 自律神経 |
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| Research Abstract |
From our previous results it is known that nitric oxide (NO) given into the cerebroventricle inhibits delivery and maternal behavior in late pregnant rats. This project has started with reexamining NO activity estimated by NADPH diaphorase histochemistry in the brain slices of the previous experiment. We found that contrary to the posterior pituitary gland , NO activity in the anterior pituitary gland is activated during the time of parturition (NeuroReport 8 : 817-821, 1997). Then we examined NO synthase by immunohistochemistiy and NADPH diaphorase histochemistry in the same tissue preparation. We found that some anterior pituitary cells which express NO synthase locate in contact with capillaries. This result indicates that NO in the anterior pituitary gland may be involved in the control of blood flow into the gland (Brain Research 784 : 337-340, 1997). In order to know the role of NO in the central nervous system to control maternal behavior, NO donor was injected into the lateral ventricle of lactating rats. As we had previously reported, milk-ejection reflex was inhibited in these rats and Fos expression in the paraventricular nucleus, supraoptic nucleus and arcuate-median eminence was suppressed. These results suggest that NO regulates activity of neurons in these nuclei to influence anterior pituitary hormones that are involved in controlling these hormonal secretion and initiation of parturition (NeuroReport and Brain Research, in press). We want to pursue the project to develop a new method to evaluate NO expression in each nucleus of the hypothalamus.
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