| Project/Area Number |
09680812
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
神経・脳内生理学
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| Research Institution | Nagoya university |
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Principal Investigator |
TATSUMI Hitoshi School of Medicine Nagata university Assistant, 医学部, 助手 (20171720)
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| Co-Investigator(Kenkyū-buntansha) |
SOKABE Masahiro Schoolof Medicine Professer, 医学部, 教授 (10093428)
片山 芳文 東京医科歯科大学, 難治疾患研究所, 教授 (20014144)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Growth Whe / evanescent / near-field / Neuron / imaging / 神経 / シナプス / ビーズ / 細胞接着 / V-DIC / 微分干渉顕微鏡 / イメージング / グルタミン酸 |
|---|
| Research Abstract |
Evanescent light illumination was introduced to a multi-imaging microscope to construct a new type of total internal reflection fluorescence microscope (TIRFM). The microscope combined TIRFM, high resolution video-enhanced differential interference contrast (DIC), epifluorescence, interference reflection (IR) with a state-of-the-art image acquisition system for time-lapse microscopy. Dynamic changes of the cell-substrate contact of the neuronal growth cone of a rat hippocampal neuron were studied with this TIRFM system. Growth cones were stained with membrane labeling fluorescence dyes (DiI or octadecyl rohdamine B) and observed using the multi-imaging capacities of this system. When growth cone regions were stimulated by pressure ejection of a high potassium solution, TIRF intensity at the basal membrane of the growth cone increased, suggesting that basal membrane of growth cone approaches the glass substrate when excited. The approach of the ventral membrane to the substrate during excitatory stimulation was also observed in IR mode. The functional importance of cell/substrate contact in growth cones is discussed.
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