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Identification and characterization of enkephalinergic neurons in spinal cord dorsal horn: Single cell RT-PCR analysis

Research Project

Project/Area Number 09680818
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionDokkyo University School of Medicine

Principal Investigator

HORI Yuuichi  Dokkyo University School of Medicine, Department of physiology, Professor, 医学部, 教授 (60190229)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
KeywordsSingle cell RT-PCR / Path Clamp Methods / Superficial dorsal horn, / Enkephalin / Reverse Transcripcion / Polymerase Chain Reaction
Research Abstract

1. We adapted the technique of single-cell reverse transcription- polymerase chain reaction (RT-PCR) for small neurons in the superficial dorsal horn of rat spinal cord slices. Nethionin-enkephalin sequence-specific PCR products were observed in more than half of superficial dorsal horn neurons studied. This is reminiscent of the previous immunohistochemical reports that a high density of enkephalin neurons are present in the superficial dorsal horn
2. Under current clamp conditions, depolarizing current injection stimulated dorsal horn neurons to generate spikes. Characteristically, enkephalinergic neurons usually displayed marked spike accommodation with a progressive prolongation of interspike intervals. In addition, enkephalinergic neurons seldom showed an initial sag upon hyperpolarizing current injection, indicating enkephalinergic neurons lack a time-dependent inwardly rectifying channel.
3. Under voltage clamp conditions, ionic currents evoked by voltage command was investigated. The following potassium currents were observed in enkephalinergic neurons: transient IA currents (in 39 of 46 enkephalinergic neurones studied); Inward rectifier ( in 18 of 39); Ca2+-dependent potassium currents (in 14 of 31); and delayed rectifier (in all enkephalinergic neurones studied).
4. Barbiturates hyperpolarized enkephalinergic neurons and suppressed spike generation in response to depolarizing currents injection.
5. It was also found that mRNA of 5-HT3 receptors was detected in some 40% of enkephalin-containing neurons. It is strongly suggested that enkephalinergic inhibitory neurons mediate Serotonin-induced spinal analgesia, owing at least partly to activation of 5-HT3 receptors expressed in enkephalinergic neurons.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Toshiaki Minami: "Involvement of primary afferent C-fibres in touch-evoked pain (allodynia) induced by prostagladin E2"European Journal of Neuroscience. 11. 1849-1856 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masaru Tsuchiya: "Enkephalinergic neurons express 5-HT3 receptors in the spinal cord dorsal horn: Single cell RT-PCR analysis"NeuroReport. 10. 2749-2753 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 堀雄一: "Bell-Magnedieの法則"脳の化学. 21. 873-876 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Megumi Shimoyama: "Gabapentin affects synaptically-evoked NMDA current in rat dorsal horn neurons"Pain. (発表予定、in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshiaki Minami, et al.: "Involvement of primary afferent C-fibres in touch-evoked pain (allodynia) induced by prostaglandin E2"European Journal of Neuroscience. vol.11. 1849-1856 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masaru Tsuchiya,et al.: "Enkephalinergic neurons express 5-HT3 receptors in the sponal cord dorsal horn: Single cell RT-PCR analysis"NeruoReport. vol.10. 2749-2753 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuuichi Hori: "Bell-Magendie's controversy (written in Japanese)"Brain Science. vol.21. 873-876 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Megumi Shimoyama, et al: "Gabapentin affects synaptically-evoked NMDA current in rat dorsal horn neurons"Pain. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigeki Yamaguchi, et al.: "Involvement of the glutamate transporter and the sodium-calcium exchanger in the hypoxia-induced increase in intracellular ca2+ in rat hippocampal slices."Brain Research. vol.813. 351-358 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigeki Yamaguchi, et al.: "Extracellular sodium concentration has diverse effects on the hypoxia-induced increase in intracellular ca2+ in rat hippicampal slices."Neuroscience Letters. vol.23. 163-166 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshiaki Minami: "Involvement of primary afferent C-fibres in touch-evoked pain (allodynia) induced by prostaglandin E_2"European Journal of Neuroscience. 11.6. 1849-1856 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masaru Tsuchiya: "Enkephalinergic neurons express 5-HT_3 receptors in the spinal cord dorsal horn : Single cell RT-PCR analysis"NeuroReport. 10.13. 2749-2753 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 堀 雄一: "Bell-Magendieの法則"脳の科学. 21.8. 873-876 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Megumi Shimoyama: "Gabapentin affects synaptically-evoked NMDA current in rat dorsal horn neuons"Pain. (発表予定、in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Tsuchiya: "Serotonin type 3 receptors are expressed in enkephalin-containing reurons in the rat spinal cord superficial dorsal horn." Japanese Journal of Physiology. Suppl.49. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Minami: "Involvement of primary afferent C-fibers in touch-evoked pain (allodynia) induced by prostaglandin E2." European Journal of Neuroscience. 11. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Yamaguchi: "Involvement of the glutamate transporter and the Na^+-Ca^<2+> exchanger in the hypoxia-induced increase in intracellular Ca^<2+> in rat hippocampal slices." Brain Research. 813・2. 351-358 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shigeki Yamaguchi: "Extracellular sodium concentration has diverse effects on the hypoxia-induced increase in intra-cellular Ca^<2+> in rat hippocampal slices." Neuroscience Letters. 232・2. 163-166 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shinpei Hanazawa: "Enhancement of formalin-induced licking response in mutant mice lacking D-amino-acid oxidase." Pain Research. 12・3. 151-151 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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