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Establishment of the osteopetrosis model in mice

Research Project

Project/Area Number 09680830
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionMie University

Principal Investigator

TSUMURA Hideki  Mie University, Faculty of Medicine, Assistant, 医学部, 助手 (20180052)

Co-Investigator(Kenkyū-buntansha) KAWANO Mitsuo  Mie University, Faculty of Medicine, Lecture, 医学部, 講師 (00234097)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsOsteoclast / FRP / Monoclonal antibody / 骨粗鬆症 / 細胞融合
Research Abstract

Murine FRP-1 (mFRP-1) and Ly10 antigens are alloantigens. Antl-Ly10.1, monoclonal antibody (mAb) reacts to HeLa cells stably expressing mFRP-1.1 (HeLa cells/mFRP-1.1), but does not react to HeLa cells! rnFRP-1.2, On the other hand, anti-Ly10.2 mAb reacts to HeLa/mFRP-1.2 cells, but does not react to HeLa cel]s/mFRP-1.1. These findings indicate that Ly10.1 and Ly10.2 antigens are identical to the heavy chains of mFRP-1.1 and rnFRP-1.2 molecules, respectively. Thymocytes and fibroblast cells obtained from CDF1 and BDFl mice showed reactivity to both anti-Ly10.1 and anti-Ly10.2/FRP-2 antibodies, indicating that mFRP-I /Ly1 0 alloantigens are codominantly expressed In the Fl mice. Surprisingly, DBT cells, derived from CDFl mice, consist of two different ceilpopulations : one expresses both mFRP-1.1 and mFRP-1.2 antigens and the other expresses only mFRP-1.1 antigen. Consequently, we tried to isolate cloned DBT cell lines by limiting dilution. Finally, six cloned DBT cell lines were obtained, and three clones of these cloned DBT cells expressed both mFRP-1.l and mFRP-1.2, and another three clones of these cloned DBT cells expressed both mFRP-1.1 and mFRP-1.2, and another three clones including clone 5 cells expressed only mFRP-L1 antigen. Neither nYFRP-1.2 mRNA nor the genomic cDNA clone encoding mFRP-1.2 could be detected in DBT clone 5 cells, nor could the genomic cDNA encoding rnFRP-1.1 be detected in IMC cells, indicating that aberrant expression of mFRP-l/LylO allotypes in CDF1 mice-derived cells is due to the gene deletlon.. The project of FRP-1 dificient mice is in progress.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Tsumura H, et al: "Mouse Alloantigen Lyic is identical to Murine Fusion Regulatory Protein/4F2/CD98" Cellular Immunology. 184. 153-160 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsumura H, et al.: "Isolation and characterization of monoclonal antibodies directed against murine FRP-1/CD98/4F2 heavy chain" Immunology and Cell Biology. 77. 19-27 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hideki Tsumura, Shoji Kimura, Mitsuo Kawano, Masato Tsurudome, Keishiro Shimura, Yasuhiko Ito: "Mouse alloantigen Ly10 is identical to murine fusion regulatory protein-1 (mFRP-1)/4F2/CD98 : Aberrant expression of mFRP-1/Ly10 allotypes in cells derived from CDF1 mice due to the gene delection" Cellular Immunology. 184. 153-160 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hideki Tsumura, Mitsuo Kawano, Masanori Tajima, Takashi Kusaura, Yuuji Kozuka, Satoshi Yoshimura, Hiroshi Komada, Masato Tsurudome, Machiko Nishio, Shigeru Kusagawa, Keishirou Shimura, Yasuhiko Ito: "Isolation and characterization oh monoclonal antibodies directed against murine FRP-1/CD98/4F2 heavy chain : Murine FRP-1 is an alloantigen and amino acid change at 129 (P<>R) is related to the alloantigenicity" Immunology and Cell Biology. 77. 19-27 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsumura H,et al.: "Mouse Alloantigon Ly10 is identical to Murine Fusion Regulatory Protein/4F2/CD98" Cellular Immunology. 184. 153-160 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsumura H,et al.: "Isolation and characterization of monodonal autibodies divected against murine FRP-1/CD98/4F2 heavy chain" Immunology and Cell Biology. 77. 19-27 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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