| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
Murine FRP-1 (mFRP-1) and Ly10 antigens are alloantigens. Antl-Ly10.1, monoclonal antibody (mAb) reacts to HeLa cells stably expressing mFRP-1.1 (HeLa cells/mFRP-1.1), but does not react to HeLa cells! rnFRP-1.2, On the other hand, anti-Ly10.2 mAb reacts to HeLa/mFRP-1.2 cells, but does not react to HeLa cel]s/mFRP-1.1. These findings indicate that Ly10.1 and Ly10.2 antigens are identical to the heavy chains of mFRP-1.1 and rnFRP-1.2 molecules, respectively. Thymocytes and fibroblast cells obtained from CDF1 and BDFl mice showed reactivity to both anti-Ly10.1 and anti-Ly10.2/FRP-2 antibodies, indicating that mFRP-I /Ly1 0 alloantigens are codominantly expressed In the Fl mice. Surprisingly, DBT cells, derived from CDFl mice, consist of two different ceilpopulations : one expresses both mFRP-1.1 and mFRP-1.2 antigens and the other expresses only mFRP-1.1 antigen. Consequently, we tried to isolate cloned DBT cell lines by limiting dilution. Finally, six cloned DBT cell lines were obtained, and three clones of these cloned DBT cells expressed both mFRP-1.l and mFRP-1.2, and another three clones of these cloned DBT cells expressed both mFRP-1.1 and mFRP-1.2, and another three clones including clone 5 cells expressed only mFRP-L1 antigen. Neither nYFRP-1.2 mRNA nor the genomic cDNA clone encoding mFRP-1.2 could be detected in DBT clone 5 cells, nor could the genomic cDNA encoding rnFRP-1.1 be detected in IMC cells, indicating that aberrant expression of mFRP-l/LylO allotypes in CDF1 mice-derived cells is due to the gene deletlon.. The project of FRP-1 dificient mice is in progress.
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