| Project/Area Number |
09680837
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kansai College of Oriental Medicine |
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Principal Investigator |
KIMURA Michio Kansai College of Oriental Medicine, Professor, 鍼灸学科, 教授 (50111745)
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| Co-Investigator(Kenkyū-buntansha) |
TOHYA Kazuo Kansai College of Oriental Medicine, Associate Professor, 鍼灸学科, 助教授 (90183491)
KURI Kiyonori Wakayama Medical College, Department of Otolaryngology, Associate Professor (40169975)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Suncus / Tubal Tonsil / Otitis Media / Animal Model / Monoclonal Antibody / Palatine Tonsil / Mucosal Immunity / Suncus IgA / 気道免疫 / M細胞 / 食虫目 / 中耳炎 |
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| Research Abstract |
A number of animal models were utilized for study of pathogenesis of Otitis Media ( OM ) using mostly conventional laboratory animals ; rat, mouce, guinea pig and chinchilla. Among these laboratory experiments, viral OM models were successfully used for influenza A virus using chin-chilla. The middle ear immunity of these animals is different from those of our human, because they lack tonsils. This did not simulate the human condition underlying reason for the chronic OM. Under such conditions, we here introduced Suncus ( suncus murinus ) a new laboratory animal which has tubal-tonsils in the middle ear and paratine ones in their fauces. Phylogenically the animal is closer to our primates. The size of suncus is almost same as those of young rats. Recently we have newly developed the anti-suncus T lymphocytes-, IgM- and IgG-monoclonal antibodies. Current data suggests that major middle ear immunoglobulins found in chronic OM include IgG, IgM and IgA, and immune protection is conerred largely by IgG and IgM. While the role of IgA in OM is not yet fully established, although thereis evidence that bacterial adherence may be prevented by secretory IgA. Delayed development in the serum bacterial antibody response is suggested to be the underlaying reason for OM susceptivility.
During the present study, we found that an anti-mouce IgA polyclonal antibody had a strong cross.
Using these IgM, IgG, IgA and T lympnocyte antibodies, the suncus middle ear immune response against artificial antigens (HRP, P6) were examined. And the obtained significant data were reorted the international symposia on animal model of OM.
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