| Project/Area Number |
09680840
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
TANAKA Kunio Asahikawa Medical College Associate Professor, 医学部, 助教授 (20041840)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Sinichi Asahikawa Medical College Professor, 医学部, 教授 (40091566)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | 31P-MRS / perfused liver / ATP / cholera toxin / forskolin / cyclic ATP / liver regeneration / intracellular signal transduction / リン-MRS / フォルスコリン |
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| Research Abstract |
The purpose of this study was to clarify the possibility to investigate the mechanism of the liver regeneration, which is the specific function of the liver, from the view point of the relation between phosphate metabolism and intracellular signal transduction by using 31P-MRS.The following results were obtained ;
1) The gradual decrease in ATP and inorganic phosphate (Pi) levels was observed dose dependently after the administration of cholera-toxin to the perfused rat liver. Significant changes in phosphomonoesters (PME) level and the ratio of ATP/Pi as the index of the energy status in the liver were not observed.
2) The decrease in ATP level was also observed after the administration of forskolin which is a unique diterpene activator of adenylate cyclase.
3) The decrease in ATP and Pi levels in the rat liver priming with an inhibitor of the protein kinase activity k-252b was significantly suppressed. The decrease in ATP and Pi levels in the rat. liver priming with the inhibitor H-89 which specifically inhibits the activation of cyclic AMP (cAMP) dependent protein kinase (Akinase) was also suppressed.
4) The concentration of cAMP in the perfusate after the perfusion of the liver was measured. The concentration of cAMP transiently increased dose dependently after the administration of cholera-toxin. The same changes in cAMP were also observed after the cholera-toxin infusion to the rat liver priming with the inhibitor of A kinase activity.
5) These results show that ATP may mainly be utilized for the protein phosphorylation after the activation of protein kinase. Following the protein phosphorylation, many kinds of chemical reactions are induced in relation to the physiologic organ functions such as the regeneration of the liver.
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