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病態局所での好中球の活性化機構とシグナル伝達

Research Project

Project/Area Number 09770104
Research Category

Grant-in-Aid for Encouragement of Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionOsaka City University

Principal Investigator

佐藤 英介  大阪市立大学, 医学部, 助手 (60211942)

Project Period (FY) 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Keywords好中球 / 活性酸素 / プライミング / 口腔内 / NF-kB / チロシンリン酸化酸素 / HL-60 / アンチセンス
Research Abstract

循環血液中の好中球の活性酸素産生能は低いが、起炎物質などでプライミングされると数十倍に活性化される。これまでに、腫瘍壊死因子(TNF-α)や顆粒球コロニー刺激因子(G-CSF)等によるプライミングにはチロシンキナーゼの活性化と115kDa蛋白チロシン残基のリン酸化が関与していることを報告してきた。今年度は口腔内好中球の、活性化に関わる因子について解析して以下の知見をえた。(1)健常人より末梢血好中球と口腔内好中球を単離し、NF-kBのp55とp65そしてIkBの発現をウエスタンブロッテングで解析した結果、いずれの好中球も発現が認められた。(2)転写活性をゲルシフトアッセイで解析した結果、無刺激では、末梢血好中球には活性が認められないが、ホーボルエステルなどで刺激すると活性化された。一方、口腔内好中球は、無刺激でも、強い活性が認められた。(3)蛍光Differential Display法を用いて、口腔内好中球に特異的に発現しているmRNAを解析したところ、数種のタンパク質の発現が見られた。これらのmRNAのうち、もっとも強く発現していたものを塩基配列を解析した結果、PAC418A9と70%のホモロジーの、未知のタンパク質である可能性が示唆された。(5)口腔内好中球は、自発性にミエロペルオキシダーゼとNOを放出するため、電気化学検出器を用いたHPLCによる解析の結果、ニトロチロシンよりもむしろクロロチロシンが生成される傾向が示されつつある。

Report

(2 results)
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Nishikawa,M.,Sato,E.F.,Kuroki,T..Utsumi.K..and Inoue.M.: "Role of Glutachione in the energy metabolism of tumor cells." Hepatology. 27. 422-426 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakanishi,K.,Nishi,Y.,Sato,E.F..Ishii.M..Hamada.T..Inoue.: "Termal injury induces thymocyte apoptosis in the rat." J.Trauma. 44・1. 143-148 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakahara,H,Sato,E.F.,Ishihara,R..Kanno.T..Yoshioka.T..: "Biochemical properties of human oral polymorphonuclear leukocytes." Free Radic.Res.28. 485-495 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishikawa,M.,Takeda,K.,Sato,E.F..Kuroki.T..Inoue.M.: "Nitric oxide regulates energy metabolism and bcl-2 expression in rat intestional epithelial cells" Am.J.Physiol.37・5. G797-801 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Morishita,S.,Sato.E.F..Takahashi.P.Y..Manabe.M.and: "Hypoglycemia-induced apoptosis in rat thymocytes in vivo." Dyabetes Reserch and Clinical Practice. 40. 1-7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamamoto,H.,Sasaki,J.,Matsuura.J..Nomura.T..Sato.: "Caracterization of the inner enamel epithelium in the enamel-free area based on the ability to secrete enamel protein demonstrated by in situ hybridization and immunohistochemistry" Acta Anatomica. 160. 232-238 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nagata,K.,Yu,H.,Nishikawa,M.,Kashiba.M..Sato.E.F..Tamura.: "Irreversible inhibition of respiration of Helicobacter pylori by nitric oxide" J.Biol.Chem.273・23. 14071-14073 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minaniyama,Y.,Takemura,S.,Tovokuni.S..Tanimoto.Y..Sato.: "Processed gain food inhibits hepatic injury in endotoxemic rats" J.Nutr.Sci.Vitaminol.31・4. 275-279 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sasaki,J.,Yamamoto,H.,Nomura,T..Matsuura.J..Seno.M..Sato.: "Multiple-labeling of oligonucleotide probes for in situ hybridization" Acta Histochem.Cytochem.31・4. 275-279 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishikawa,M.,Sato.E.F.,Kuroki,T..Utsumi.K..Inoue.M.: "Macrophage-derived Nitric oxide induces apoptosis of ascites hepatoma cells in vivo" Hepatology. 28・6. 1474-1480 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishikawa,M.,Sato,E.F.,Utsumi,K.,Inoue,M.: "Biological Oxidants and Antioxidants:Molecular Mechanisms and" AOCS PRESS, 54-64 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Utsumi,K.,Takehara,Y.,Inai,Y.,Yabuki,M.,Kanno,T.,Nishikawa,M.,Sato,EF.,Inoue,M.: "Mitochondria,Oxidants,and Aging" Academic Press, 57-72 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishikawa,M.;Sato,E.F.;Kuroki,T.;Inoue,M.: "Role of glutathione and nitric oxide in the energy metabolism of rat liver mitochondria" FEBS Lett.415(3). 341-5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamasu,S.:Sato,E.F.;Ogita,S.;Inoue,M.: "Role of glutathione metabolism and apoptosis in the regression of liver hemopoiesis" Free-Radic-Biol-Med. 23(1). 100-9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yu,H.;Sato,E.F.;Nagata,K.;Nishikawa,M.;Kashiba,M.;Arakawa,: "Oxygen-dependent regulation of the respiration and growth of Escherichia coli by nitric oxide" FEBS Lett.409(2). 161-5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yu,H.;Sato,E.F.;Minamiyama,Y.;Arakawa,T.;Kobayashi,K.;Inoue,M.: "Effect of nitric oxide on stress-induced gastric mucosal injury in the rat" Digestion. 58(4). 311-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shiraishi,I.;Sato,E.F.;Nishikawa,M.;Sendo,F.;Inoue,M.: "Mechanism of hypotensive shock induced by anti-neutrophil monoclonal antibody" Pathophysiology. 4:. 197-204 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morishita,S.;Nishi,Y.;Sato,E.F.;Manabe,M.;Yamaoka,K.;Inoue,M.: "Cold-induced apoptosis in rat thumocytes in vivo" Pathophysiology. 4. 213-219 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nishikawa,M.;Sato,E.F.;Kashiba,M.;Kuroki,T.;Utsumi,K.;Inoue,M.: ":Role of glutathione and nitric oxide-dependent regulation of energy metabolism in rat hepatoma cells" Hepatology. 27. 422-426 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakanishi,K.;Nishi,Y.;Sato,E.F.;Ishii,M.;Hamada,T.;Inoue,M.: "Termal injury induces thymocyte apoptosis in the rat" J.Trauma. 44(1). 143-148 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakahara,H.;Sato,E.F.;Ishihara,R.;Yabuki,M.;Inoue,M.;Utsumi,K.: "Biochemical Properties of human oral neutrophils" Free Radic.Res.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nishikawa,M.;Takeda,K,;Sato,E.F.;Kuroki,T.;Inoue,M.: "Nitric oxide regulates energy metabolism and bcl-2 expression in rat intestional epithelial cells" Am.J.Physiol.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morishita,S.;Nishi,Y.;Sato,E.F;Manabe,M.;Yamaoka,K.;Inoue,M.: "Hypoglycemia-induced apoptosis in rat thymocytes in vivo" Dyabetes Res.Clin.Prac.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagata,K.;Yu,H.;Kashiba,M.;Nishikawa,M.;Sato,E.F.;Utsumi,K.: "Irreversible inhibition of respiration of Helicobacter pylori by nitric oxide" J.Biol.Chem.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto,H.;Sasaki,J.;Matsuure,J.;Momura T.;Sato,E.F.;Shimokawa,H.: "Characterization of the inner enamel epithelium in the enamel-free area based on the ability to secrete enamel protein demonstrated by in situ hybridization" Acta Anatomica. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Minamiyama,Y.,Sato,Ef.,Takemura,S,,Inoue.M..: "Food and Free Radicals" Hiramatsu, M.Plenum press,New York, 137-140 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kashiba-Iwatsuki,M.,Kitoh,K.,Nishikawa,M..Sato,E.F.,Inoue.: "Oxygen Homeostasis and its dynamics" Ishimura,Y.,Shimada,H.,Springer-Verlag,Tokyo, 562-568 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Utsumi,K.,Takehara,Y.,Inai,Y.,Yabuki,M.,Kanno.T..Nishikawa.: "Mitochondria,Oxidants,and Aging" Packer,L.,Cadenas Academic Press, (in press) (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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