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Morphology and physiology of masticatory neurons in the trigeminal principal nucleus and supratrigemenal nucleus -intracellular recording and HRP injection technique-

Research Project

Project/Area Number 09832007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 咀嚼
Research InstitutionOsaka University

Principal Investigator

YOSHIDA Atsushi  Osaka Univ., Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (90201855)

Co-Investigator(Kenkyū-buntansha) NAGASE Yoshitaka  Osaka Univ., Faculty of Dentistry, Research Associate, 歯学部, 助手 (50252698)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsmastication / trigeminal / secondary neuron / cat / Nucleus principalis / jaw reflex / HRP / intracellular recoding / 細胞内電位記録法
Research Abstract

Although the principalis nucleus (Vp) contains trigeminothalamic and internuclear tract cells, the functional and morphological differences between the two kinds of neurons have remained unsettled.The present study was aimed to address these problems by using the intracellular horseradish peroxidase (HRP) injection technique in the cat.Of 20 neurons stained, seven and thirteen were located in the dorsomedial subnucleus (Vpd) and ventrolateral subnucleus (Vpv) of Vp, respectively.The Vpd neurons received input from the intraoral structures only but the Vpv neurons from the intraoral or facial structures.Nineteen neurons could be divided as class I and class II, based on the branching pattern of their stem axons Class I (eight neurons) had an ascending stem axon without branching.Class II was divided into two subclasses (IIa and IIb). Class IIa (eight neurons) had an ascending stem axon from which branches were given off.Their branches formed a local-circuit restricted to the lower brainstem.Class IIb (three neurons) had a stem axon that formed the local-circuit only.The dendritic morphology was indistinguishable between different classes of neurons and between the subdivisions.Although the dendritic arborization pattern was governed by the location of the somata, it was suggested to be also important elements for determining primary affrent arborizations.In the brainstem nuclei, the jaw-closing motor nucleus received the highest density of projections from class II neurons with the receptive field involving the periodontal ligaments.
The present study provided new findings that Vp neurons could be divided into three distinct populations, and suggests that each population exerts a distinct function with respect to sensory discrimination and/or sensorimotor reflexes.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Yoshida,A.: "Physiological and morphological properties of motoneurons and primary and secondary spindle afferents innervating the temporalis in the cat." J.Comp.Neurol.(印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukunishi,Y.: "Quantitative analysisi of the dendritic architectures of cat hypoglossal motoneurons stained intracellularly with horseradish peroxidase." J.Comp.Neurol.(印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida,A.: "Morphologic characteristics of physiologically defined neurons in the cat trigeminalnucleus principalis." J.Comp.Neurol.401. 308-328 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Moritani,M.: "Morphological differences of fast and slowly adapting lingual afferent terminations in the principal and oral nuclei in the cat." J.Comp.Neurol.396. 64-83 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kishimoto,H.: "The central distribution of synaptic contacts of primary and secondary jawmuscle spindle afferents in the trigeminal motor nucleus of the cat." J.Comp.Neurol.391. 50-63 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida,A.: "Neurobiology of Mastication-From Molecular to Systems Approach" Y.Nakamura (ed.),Excerpta Medica International Congress Series Volume number 1186,Elsevier Science (印刷中), (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigenaga,Y.: "Neurobiology of Mastication-From Molecular to Systems Approach" Y.Nakamura (ed.),Excerpta Medica International Congress Series Volume number 1186,Elsevier Science (印刷中), (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kishimoto, H., Y.C.Bae, A.Yoshida, M.Moritani, M.Takemura, S.Nakagawa, Y.Nagase, T.Wada, B.J.Sessle and Y.Shigenaga: "The central distribution of synaptic contacts of primary and secondary jaw-muscle spindle afferents in the trigeminal motor nucleus of the cat." J.Comp.Neurol.391. 50-63 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Moritani, M., A.Yoshida, S.Honma, Y.Nagase, M.Takemura and Y.Shigenaga: "Morphological differences of fast and slowly adapting lingual afferent terminations in the principal and oral nuclei in the cat." J.Comp.Neurol.396. 64-83 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida, A., T.Hiraga, M.Moritani, K.Chen, Y.Takatsuki, Y.Hirose, Y.C.Bae and Y.Shigenaga: "Morphologic characteristics of physiologically difined neurons in the cat trigeminal nucles principalis." J.Comp.Neurol.401. 308-328 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukunishi, Y., Y.Nagase, A.Yoshida, M.Moritani and Y.Shigenaga: "Quantitative analysisi of the dendritic architectures of cat hypoglossal motoneurons stained intracellularly with horseradish peroxidase" J.Comp.Neurol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida, A., N.Mukai, M.Moritani, Y.Nagase, Y.Hirose, T.Matsuya and Y.Shigenaga: "Physiological and morphological properties of motoneurons and primary and secondary spindle afferents innervating the temporalis in the cat." J.Comp.Neurol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida, A., K.Yasuda, Y.C.Bae, M.Moritani, Y.Hirose, H.Fukami, K.Chen, B.J.Sessle and Y.Shigenaga: "Neurobiology of Mastication-From Molecular to Systems Approach Principalis and oralis premotoneurons for the cat trigeminal motor nucleus." Excerpta Medica International Congress Series Volume number 1186, Y.Nakamura (ed.), Elsevier Science. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigenaga, Y., A.Yoshida, H.Kishimoto, N.Mukai, M.Moritani, Y.Nagase and Y.C.Bae: "Morphologies of masticatory motoneurons and muscle spindle afferents" Excerpta Medica International Congress Series Volume number 1186, Y.Nakamura (ed.), Elsevier Science. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Bae, Y.C., K.P.Park, H.J.Ihn, A.Yoshida, S.Honma and Y.Shigenaga: "Glutamate, GABA,and glycine immunoreactive terminals on cat masseter motoneurons." Excerpta Medica International Congress Series Volume number 1186, Y.Nakamura (ed.), Elsevier Science. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Honma, S., M.Moritani, L.F.Zhang, A.Yoshida and Y.Shigenaga.: "Ultrastructures of axon terminals contacting periodontal and spindle afferents." Excerpta Medica International Congress Series Volume number 1186, Y.Nakamura (ed.), Elsevier Science. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagase, Y., T.Nakamura, M.Moritani, S.Honma, A.Yoshida and Y.Shigenaga: "A quantiative analysis of the dendritic organization of cat trigeminal motoneurons." Excerpta Medica International Congress Series Volume number 1186, Y.Nakamura (ed.), Elsevier Science. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida,A.: "Physiological and morphological properties of motoneurons and primary and secondary spindle afferents innervating the temporalis in the cat." J.Comp.Neurol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukunishi,Y.: "Quantitative analysis of the dendritic architectures of cat hypoglossal motoneurons stained intracellularly with horseradish peroxidase." J.Comp.Neurol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida,A.: "Morphologic characteristics of Physiologically defined neurons in the cat trigeminal nucleus principalis." J.Comp.Neurol.401. 308-328 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Moritani,M.: "Morphological differences of fast and slowly adapting lingual afferent terminations in the principal and oral nuclei in the cat." J.Comp.Neurol.396. 64-83 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kishimoto,H.: "The central distribution of synaptic contacts of primary and secondary jaw-muscle spindle afferents in the trigeminal motor nucleus of the cat." J.Comp.Neurol.391. 50-63 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida,A.: "Neurobiology of Mastication-From Molecular to Systems Approach" Y.Nakamura(ed.),Excerpta Medica International Congress Series Volume number 1186,Elsevier Science(in press), (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shigenaga,Y.: "Neurobiology of Mastication-From Molecular to Systems Approach" Y.Nakamura(ed.),Excerpta Medica International Congress Series Volume number 1186,Elsevier Science(in press), (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.KISHIMOTO: "THE CENTRAL DISTRIBUTION OF SYNAPTIC CONTACTS OF PRIMARY AND SECONDARY JAW-MUSCLE SPINDLE AFFERENTS IN THE TRIGEMINAL MOTOR NUCLEUS OF THE CAT" J.Comp.Neurol.391. 50-63 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.C.BAE: "IDENTIFICATION OF γ-AMINOBUTYRIC ACID-IMMUNOREACTIVE AXON ENDINGS ASSOCIATED WITH MESENCEPHALIC PERIODONTAL AFFERENT TERMINALS AND MORPHOMETRY OF THE TWO TYPES OF TERMINALS IN THE CAT SUPRATRIGEMINAL NUCLEUS" J.Comp.Neurol.389. 127-138 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉田 篤: "目でみる脳科学、三叉神経[IV]-二次ニューロンから運動ニューロンへの投射-" ブレインサイエンス. 8. 355-358 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉田 篤: "目でみる脳科学、三叉神経[V]-セロトニン作動性線維の運動ニューロンへの投射-" ブレインサイエンス. 8. 233-237 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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