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CHANGE IN SPATIAL MEMORY AND HIPOCAMPAL EXPRESSION OF C-FOS PROTEIN AS A RESULTS OF IMPAIRED MASTICATION IN THE SENSCENCE-ACCELERATED MOUSE

Research Project

Project/Area Number 09832012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 咀嚼
Research InstitutionKanagawa Dental College

Principal Investigator

NISHIYAMA Katsuhiro  Kanagawa Dental College, DENTISTRY ASSISTANT PROFESSOR, 歯学部, 講師 (20084783)

Co-Investigator(Kenkyū-buntansha) WATANABE Kazuko  GIF UNIVERSITY MEDICINE ASSISTANT PROFESSOR, 医学部, 講師 (40158621)
SAITO Shigeru  Kanagawa Dental College, DENTISTRY PROFESSOR, 歯学部, 教授 (80084713)
川瀬 俊夫  神奈川歯科大学, 歯学部, 教授 (30084784)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsHippocampal / mastication / oncogene / MRI / c-Fos / Morris water maze / human / brain derived factor / 記憶 / アセチルコリン / 神経
Research Abstract

In the present study, we evaluated the effect of reduced mastication on the neuronal mechanism responsible for age-related memory impairment, by examining the effects of the cutting off of the upper molars (molarless) on hippocampal expression of c-fos protein (c-Fos) and spatial memory in the senescence-accelerated mouse (SAM-P8) and the senescence-resistant mouse (SAM-R1). In the Morris water maze test, the escape latency for intact SAM-P8 to find the hidden platform was longer in the first several days and thereafter almost the same as that for the SAM-R1 control. Immunohistochemical analysis following this behavioral test (on day 10) revealed no significant density of c-Fos-immunopositive cell nuclei in the hippocampal formation between these two groups. However, in molarless SAM-P8, both a marked increase in escape latency in the water maze and a significant decrease in c-Fos-immunoposive nuclei density were seen, the greatest effect being found in the CAl subfield, followed by the CA3 subfield, then the dentate gyrus. These changes became more pronounced with the increasing duration of the molarless condition. In contrast, the molarless SAM-R1 did not show any significant changes in maze leaning and c-Fos expression. Furthermore, the molarless condition had no effect on GFAP-positive cell density in the hippocampal formation in either SAM-R1 or SAM-PS mice. These results suggest that impaired mastication may cause the age-related reduction in hippocampal activity, linked to impairment of memory.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Watanabe, K.,: "A novel glutamate-mediated inhibitory mechanism linked with Ca^<2+>/calmodulin-dependent protein kinase II in identified Euhadra neurons." J.Neurobiol.32. 139-149 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe, K.,: "Decrease in the learning and memorial ability induced by reduction of masticatory movement in the senescence-accelerated mouse." Nerrosci, Res. 21. S178-179 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Karasawa, N: "Age-related changes in the catecholamine biosynthesis system in the brain of senescence-accelerated mouse-prone (SAM-P/8)." Neurosci, Res. 21. S180-181 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Karasawa, N: "Immunocytochemical study of catecholaminergic neurons in the senescence accelerated (SAM-P8) mouse brain." J.Neural Transm. 104. 1267-1275 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Onozuka, M: "Glial reaction linked with P70-inducced seizure activitiy in the cerebral cortex of rats." Neurosci, Res, Commun. 23. 111-117 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe, K., Yamamamoto, T., Ozono, S., Nagasaki, S., and Onozuka, M: "A novel glutamate-mediated inhibitory mechanism linked with Ca2+/calmodulin-dependent protein kinase II in identified Euhadra neurons." J.Neurobiol.32. 139-149 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe, K., Nagasaki, S., Yamamoto, T., Karasawa, N., Nagatsu, I., and Onozuka, M.: "Decrease in the learningand memorial ability induced by reduction of masticatory movement in the senescence-accelerated mouse." Neurosci.Res.(Suppl.). 21. S178 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Karasawa, N., Nagatsu, I., Watanabe, K., and Onozuka, M.: "Age-related changes in the catecholamine biosynthesis system in the brain of senescence-accelerated mouse-prone (SAM-P/8)." Neurosci.Res.(Suppl.). 21. S180 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Karasawa, N., Nagatsu, I., Sakai, K., Nagatsu, Y., Watanabe, K., and Onozuka, M.: "Immunocytochemical study of catecholaminergic neurons in the senescence accelerated (SAM-P8) mouse brain." J.Neural Transm.104. 1267-1275 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Onozuka, M., Watanabe, K., H,Takada., SM,Mirbod., Karasawa, N., Nagatsu, I., Nishiyama, K., and Ozono, S.: "Glial reaction linked with P70-inducced seizure activitiy in the cerebral cortex of rats." Neurosci.Res.Commun.23. 111-117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe, K., Nishiyama, K., Saito, S., Ozono, S., Kubo, K., Karasawa, N., Nagatsu, I., and Onozuka, M.: "Changes in spatial memory and hippocampal expression of c-fos protein as a result of impaired mastication in the senescence-accelerated mouse." Neurobiol.Aging.(Submitted.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe,K.,: "A novel glutamate-mediated inhibitory mechanism linked with Ca^<2+>/calmodulin-dependent protein kinase II in identified Euhadra neurons." J.Neurobiol.32. 139-149 (1979)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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