| Project/Area Number |
09836006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
免疫の制御機構
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
WAKATSUKI Yoshio Kyoto University, Faculty of Medicine, Lecturer, 医学研究科, 講師 (40220826)
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| Co-Investigator(Kenkyū-buntansha) |
KUDO Akira Tokyo Inst. Technology, Science, Professor, 生命理工学部, 教授 (70178002)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Immunology / B cell / differentiation / antibody / transcription factor / transgenic mouse |
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| Research Abstract |
In order to determine in vivo function of Pax-5/BSAP, function in the regulation of antibody secretion and B cell differentiation in particular, we investigated genomic configuration of Pax-5 gene and specificity of Pax-5 promotor activity in B cells. In addition, we have created transgenic mouse in which physiological regulation of Pax-5 expression is abrogated. By doing this, we studied the effect of sustained over expression of Pax-5 at the B cell differentiation to plasma cell, the differentiation stage at which native Pax-5 expression is turned off.
1, We have cloned a DNA fragment of 2Kb which spans exon 1A of Pax-5 and 5' intronic region.
2, No B cell specific regulatory elements were found in this region.
3, Novel region regulating B cell specific expression of Pax-5 is identified.
4, Novel transcription factors regulating Pax-5 expression was discovered.
5, Pax-5 transgenic mice were established. Unexpected phenotype of the mice was identified.
The project is now at the stage of publication.
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