• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Origin and Evolution of the Vertebrate Immune System

Research Project

Project/Area Number 09839029
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 自然史科学
Research InstitutionThe University of Tokyo (1998)
Nagoya City University (1997)

Principal Investigator

NONAKA Masaru  University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (40115259)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsComplement / MHC / Innate Immunity / Ascidian / Medaka / 補体 / ホヤ
Research Abstract

1) Molecular cloning and structural analysis of ascidian complement components :
cDNA clones for complement component MASP and C3 were isolated from the liver library of Japanese ascidian, Halocynthia roretzi. The deduced primary structures of these component indicated that they are ascidian counterparts of vertebrate MASP and C3. Antibodies against ascidian C3 inhibited an opsonic activity of ascidian body fluid to enhance phagocytosis of yeast by ascidian coelomocytes. These results indicate that the multi-component, opsonic complement system has been established by the time of urochordates emergence, much earlier than adaptive immunity.
2) Linkage analysis of mammalian MHC-encoded genes in Xenopus and Medaka fish :
Most mammalian MHC-encoded genes tested linked to each other in Xenopus, indicating the conservation of this linkage group between amphibians and mammals. In contrast, MHC class I, II and III genes showed a dispersed distribution all over Medaka fish chromosomes, except for a close linkage among the class IA, LMP2, LMP7 and TAP2 genes. Thus the class IA and its antigen processing/transport genes seem to be the core part of the MHC, indicating that the original physiological meaning of the MHC was to establish the class I antigen presentation system.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Salter-Cid,L.: "Expression of MHC class Ia and class Ib during ontogeny : high expression in epithelia and co-regulation of class Ia and lmp7 genes" J.Immunol.160. 2853-2861 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakao,M.: "Two diverged complement factor B/C2-like cDNA sequences from a teleost,carp (Cyprinus carpio)" J.Immunol.161. 4811-4818 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo,Y.: "Two lineages of mannose binding lectin-associated serine protease (MASP) in vertebrate" J.Immunol.161. 4924-49〓 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nonaka,M.: "Molecular genetics of the complement C3 convertases in lower vertebrates" Immunol.Rev.166. 59-65 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nonaka,M.: "Opsonic complement component C3 in the solitary ascidian,Halocynthia roretzi" J.Immunol.162. 387-391 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Salter-Cid, L., Nonaka, M.& Flajnik, M.F.: "Expression of MHC class Ia and class Ib during ontogeny : high expression in epithelia and co-regulation of class Ia and Imp7 genes." J.Immunol.160. 2853-2861 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakano, M., Fushitani, Y., Fujiki, K., Nonaka, M.& Yano, T.: "Two diverged complement factor B/C2-like cDNA sequences from a teleost, carp (Cyprinus carpio)." J.Immunol.161. 4811-4818 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo, Y., Takahashi, M., Nakao, M., Saiga, H., Sekine, H., Matsushita, M., Nonaka, M.& Fujita, T.: "Two lineages of mannose binding lectin-associated serine protease (MASP) in vertebrate." Immunol.161. 4924-4930 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nonaka, M., Kuroda, N., Naruse, K.& Shima, A.: "Molecular genetics of the complement C3 convertases in lower vertebrates." Immunol.Rev.166. 59-65 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nonaka, M., Azumi, K., Ji, X., Namikawa-yamada, C., Sasaki, M., Saiga, H., Dodds, A.W., Sekine, H., Homma, M.K., Matsushita, M., Endo, Y.& Fujita, T.: "Opsonic complement component C3 in the solitary ascidian, Halocynthia roretzi." J.Immunol.162. 387-391

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Salter-Cid,L.: "Expression of MHC class Ia and class Ib during ontogeny : high expression in epithelia and co-regulation of class Ia and lmp7 genes" J.Immunol.160. 2853-2861 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakao,M.: "Two diverged complement factor B/C2-like cDNA sequences from a teleost,carp(Cyprinus carpio)" J.Immunol.161. 4811-4818 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo,Y.: "Two lineages of mannose binding Lectin-associated serine protease(MASP)in vertebrate" J.Immunol.161. 4924-4930 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nonaka,M.: "Molecular genetics of the complement C3 convertases in lower vertebrates" Immunol.Rev.166. 59-65 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nonaka,M.: "Opsonic complement component C3 in the solitary ascidian,Halocynthia roretzi" J.Immunol.162. 387-391 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nonaka,M., Namikawa,C., Kato,Y., Sasaki,M., Satlar-Cid,L., Flajnik,M.: "Major hisdocompatibility complex gene mapping in the amphibian Xenopus implies a primordial organization." Proc.Natl.Acad.Sci.USA. 94. 5789-5791 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Namikawa,C., Naruse,K., Wada,H., Shima,A., Kuroda,N., Nonaka,M., Sasaki,M.: "Genetic linkage between the LMP2 and LMP7 genes in didalea fish,a teleost." Immunogenetics. 46. 431-433 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ji,X., Azumi,K., Sasaki,M., Nonaka,M.: "Ancient origin of the complement lectin pathway revealed by molecular cloning of woman binding protein-associated serine protease fian aurochedats the Japanese ascidian, Halocynthia roretzi" Proc.Nadl.Acad.Sci.USA. 94. 6340-6345 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nonaka,M., Namikawa,C., Sasaki,M., Salter-Cid,L., Flajnik,M.: "Evolulion of proteasome subunits S and LMP2 ; cDNA cloning and linkage analysis with MHC in lower vertebrates" J.Immunol.159. 734-740 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi