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Phylogenetic Relationships of Giant Salamander Species by Means of Analyzing Genomic Highly Repetitive DNA.

Research Project

Project/Area Number 09839035
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 自然史科学
Research InstitutionTOHO UNIVERSITY

Principal Investigator

KOHNO Sei-ichi  Toho University Science Professor, 理学部, 教授 (70057644)

Co-Investigator(Kenkyū-buntansha) KUBOTA Souichirou  Toho University Science Assistant, 理学部, 助手 (30277347)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsAndrias / Cryptobranchus / Cryptobranchid / Hin dIII / 5S rDNA / DNA sequence / transversion / repetitive DNA / オオサンショウウオ / チュウゴクオオサンショウウオ / アメリカオオサンショウウオ / 55rDNA / 塩素配列 / 種内変異 / 種間変異 / Hin dIII
Research Abstract

A living fossil, the giant salamander (Cryptobranchid) now inhabits in Japan, China and USA and is classified into two genera (Andrias and Cryptobranchus) and three species (A.japonicus, A.davidianus and C.alleganiensis) by morphological characteristics. The classification is very difficult and even a taxonomist of Urodela can not classify sometimes. The scientific data concerning this giant salamanders are poor and are required for preservation of them.
We tried to clarify the phylogenetic relationships of those three giant salamander species by means of analyzing the sequences of two genomic repetitive DNA.These are the fragments (about 120 bp) digested the genome DNA by a restriction enzyme, Hin dIII, and 5S rRNA genes (about 120 bp) with nontranscribed sparser (about 350 bp), from 8 and 7 specimens (A.j. : 5 and 4, A.d. : 2, C.a. : 1), respectively.
By use of the fragments digested by Hin dIII, we clarified divergence of two genera, but failed to clarify divergence of the two species in the genus Andrias. On the other hand, by use of 5S rDNA with nontranscribed spacer, we clarified the divergence not only the two genera but also the two species In the genus Andrias. The genetic distance of 5S rDNA in these species suggests that differentiations should be happened in these three species. We clarified that all four specimens of A.japonicus examined bear a C to G transversion at a 52-base mononucleotide tract in the 5S rRNA gene. We also found 7 substitutions in 5S rRNA gene with spacer, specific to C.alleganiens is. It should be the distinct molecular characteristics of these species.
We could not point out clearly the intraspecific variations according to the locality in two species. A.japonicus and A.davidianus.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Honma, Takako: "Analysis of phylogenetic relationships of three giant salamander species by means of highly repetitive DNA." Genes Genet.Syst.72. 415 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 本間貴子: "反復配列を指票としたオオサンショウウオ科3種の系統解折" 東邦大学理学研究科博士前期課程論文(1997年度), 40 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山浦秀介: "オオサンショウウオ科3種の5S rDNAを指標とした系統関係の推定" 東邦大学理学部生物学科卒業論文(1998年度), 19 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Honma, T., Tamamoto, H., Kuro-o, M., Kubota, S., Kohno, S.: "Analysis of phylogenetic relationships of three giant salamander species by analysis of highly repetitive DNA." Genes Genet.Syst.72. 415 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Honma, T.: "Molecular genetic analysis of highly repetitive DNA isolated from three species in the family Cryptobrachidae." Thesis of Master of Science, Toho University, Japan. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaura, S.: "Molecular genetic analysis of 5S rDNA from three species in the family Cryptobranchidae." Thesis of Bachelor of Science, Toho University, Japan. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Honma,Takako: "Analysis of phylogenetic relationships of three giant salamander specles by means of highly repetitive DNA." Genes Genet.Syst.72. 415 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 本間貴子: "反復配列を指標としたオオサンショウウオ科3種の系統解析" 東邦大学理学研究科博士前期課程論文(1997年度), 40 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山浦秀介: "オオサンショウウオ科3種の5SrDNAを指票とした系統関係の推定" 東邦大学理学部生物学科卒業論文(1998年度), 19 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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