細胞内情報伝達機構網の研究

• Project/Area Number: 09NP0601
• Research Category: Grant-in-Aid for Creative Basic Research
• Allocation Type: Single-year Grants
• Research Institution: Kobe University
• Principal Investigator: 西塚 泰美 神戸大学, 名誉教授 (10025546)
• Co-Investigator(Kenkyū-buntansha): 米澤 一仁 神戸大学, バイオシグナル研究センター, 教授 (70283900) ; 斎藤 尚亮 神戸大学, バイオシグナル研究センター, 教授 (60178499) ; 吉川 潮 神戸大学, バイオシグナル研究センター, 教授 (40150354) ; 小野 功貴 神戸大学, バイオシグナル研究センター, 教授 (10243297) ; 深見 泰夫 神戸大学, 遺伝子実験施設, 助教授 (00156746) ; 向井 秀幸 神戸大学, 自然科学研究科, 助教授 (80252758) ; 横山 光宏 神戸大学, 医学部, 教授 (40135794) ; 米原 伸 京都大学, ウイルス研究所, 教授 (00124503) ; 福井 泰久 東京大学, 大学院・農学生命科学研究科, 教授 (00181248) ; 山村 博平 神戸大学, 医学部, 教授 (90030882) ; 井上 圭三 東京大学, 大学院・薬学系研究科, 教授 (30072937) ; 淀井 淳司 京都大学, ウイルス研究所, 教授 (80108993)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Keywords: プロテインキナーゼC / 脂質メッセンジャー / ターゲッティング / PKN / CG-NAP / アミノ酸バランス / mTOR / チロシンキナーゼ / 情報伝達機構網 / 構造解析 / 創薬 / PAF-アセチルヒドロラーゼ / PLD(ホスホリパーゼD) / PLC(ホスホリパーゼC) / PKC(プロテインキナーゼC) / 核-細胞質間物質輸送 / PI 3キナーゼ / Fas / NO(一酸化窒素) / ホスホリパーゼA / 低分子量G蛋白質 / ストレス / ホスファチジルイノシトール3キナーゼ / 核局在化シグナル / チオレドキシン / 一酸化窒素合成酵素

Research Abstract

平成12年度に以下の成果をあげた。
1 プロテインキナーゼC(PKC)と情報クロストーク機構について
(1)PKCδ分子種のチロシンリン酸化の誘導におけるチロシンキナーゼの働き、(2)質量分析法によるPKCδ分子種のチロシンリン酸化部位の決定、(3)PKCδ分子種のチロシンリン酸化を介する活性制御、(4)PKCδ分子種を介するアポトーシス誘導とPKBとの拮抗作用、について明らかにした。
2 脂質メッセンジャーとPKCのターゲッティング機構について
(1)phospholipase A2によって産生された脂肪酸やリゾリン脂質のPKCターゲッティングへの関与、(2)基質蛋白質のリン酸化におけるPKCターゲッティングの必要性、(3)脂質メッセンジャー(アラキドン酸、セラミドなど)によるPKCターゲッティングと活性化機構、(4)ジアシルグリセロールキナーゼ(DGK)のターゲッティングによるPKCシグナリングの制御機構、を明らかにした。
3 PKC関連リン酸化酵素と細胞機能の調節について
(1)PKNと相互作用する蛋白質として、CG-NAP(centrosome and Golgi localized PKN-associated protein)を同定した。また、PKNが神経特異的転写因子NDRF/NeuroD2、癌遺伝子産物E6蛋白質に結合し、機能を制御することを見出した。(2)新規のPKNファミリーであるPKNβを同定した。(3)細胞周期制御におけるPKNの機能解析を行った。
4 病態におけるPKCシグナリングの解析について、
(1)アミノ酸バランスによるp70S6キナーゼの活性制御機構、(2)アミノ酸-mTORシグナル系とPKCの関連、を明らかにした。
5 ターゲッティングと細胞周期の調節
(1)A431細胞におけるShcおよびc-Src活性化とトランスロケーション、(2)受精におけるSrc型チロシンキナーゼの役割、を明らかにした。

Research Products

• [Publications] Konishi,H.: "Tyrosine phosphorylation sites of protein kinase C δ in the H_2O_2-treated cells and the regulation of its catalytic activity in vitro."Proc.Natl.Acad.Sci.USA. (印刷中).
• [Publications] Yamamoto,T.: "H_<>O_<>-induced tyrosine phosphorylation of protein kinase C δ by a mechanism independent of inhibition of protein-tyrosine phosphatase in CHO and COS-7 cells."Biochem.Biophys.Res.Commun.. 273・3. 960-966 (2000)
• [Publications] Okuno,S.: "Studies on the phosphorylation of protein kinase B by Ca^<2+>/calmodulin-dependent protein kinases."J.Biochem.. 127・6. 965-970 (2000)
• [Publications] Kitaura,J.: "Akt-dependent cytokine production in mast cells."J.Exp.Med.. 192・5. 729-740 (2000)
• [Publications] Oka,M.: "Regulation of melanogenesis through phosphatidylinositol 3-kinase-Akt pathway in human G361 melanoma cells."J.Invest.Dermatol.. 115・4. 699-703 (2000)
• [Publications] Oyasu,M: "Immunocytochemical localization of a neuron-specific thrombospondin-1-like protein, NELL2 : Light and electron microscopic studies in the rat brain."Mol.Brain Res.. 76. 151-160 (2000)
• [Publications] Sumioka,K.: "Induction of 55 kDa PKN cleavage product by ischemia/reperfusion model in the rat retina."Invest.Opthal.Vis.Sci.. 41・1. 29-35 (2000)
• [Publications] Sakai,N.: "Involvement of the actin cytoskeleton in the regulation of serotonin transporter (SET) activity : possible mechanism underlying SET regulation by protein kinase C."Neurochem.Int.. 36. 567-579 (2000)
• [Publications] Shirai,Y.: "Phospholipase A_2 and its products are involved in the purinergic receptor-mediated translocation of protein kinase C in CHO-K1 cells."J.Cell Sci.. 113. 1335-1343 (2000)
• [Publications] Akiyama,H.: "Blood-brain barrier formation of grafted human umbilical vein endothelial cells in athymic mouse brain."Brain Res.. 858. 172-176 (2000)
• [Publications] Tanii,H.: "Involvement of GABA neurons in allylnitrile-induced dyskinesia."Brain Res.. 887. 454-459 (2000)
• [Publications] Ueyama,T.: "cDNA cloning of an alternative splicing variant of protein kinase Cδ(PKCδIII), a new truncated form of PKCδ, in rats."Biochem.Biophys.Res.Commun.. 269・2. 557-563 (2000)
• [Publications] Shirai,Y.: "Subtype-specific translocation of diacylglycerol kinase α and γ and its correlation with protein kinase C."J.Biol.Chem.. 275・32. 24760-24766 (2000)
• [Publications] Nakagawa,N.: "ENH, containing PDZ and LIM domain, heart/skeletal muscle-specific protein, associates with cytoskeletal protein through its PDZ domain."Biochem.Biophys.Res.Commun.. 272. 505-512 (2000)
• [Publications] Shibaguchi,H.: "Role of synaptophysin in exocytic release of dopamine from Xenopus oocytes injected with rat brain mRNA."Cellular and Molecular Neurobiology. 20. 401-408 (2000)
• [Publications] Ohmori,S.: "Importance of PKC targeting for the phosphorylation of its substrate, myristoylated alanine-rich C-kinase substrate (MARCKS)."J.Biol.Chem.. 275・34. 26449-26457 (2000)
• [Publications] Larsen,E.C.: "Differential requirement for classic and novel PKC isoforms in respiratory burst and phagocytosis in RAW 264.7 cells."J.Immunol.. 165. 2809-2817 (2000)
• [Publications] Yokogawa,T.: "Evidence that 3'-phosphorylated polyphosphoinositides are generated at the nuclear surface : use of immunostaining technique with monoclonal antibodies specific for PI 3,4-P_2."FEBS Lett.. 473. 222-226 (2000)
• [Publications] Kondoh,T.: "In vivo gene transfer into periventricular region by electroporation."Neurologia medico-chirurgica.. 40. 618-623 (2000)
• [Publications] Kajimoto,T.: "Subtype-specific translocation of the d subtype of protein kinase C and its activation by tyrosine phosphorylation induced by ceramide in HeLa cells."Mol.Cell.Biol.. 21. 1769-1783 (2001)
• [Publications] Nakai,M.: "Amyloid β protein activates PKC-δ and induces translocation of myristoylated alanine-rich C kinase substrate (MARCKS) in microglia."Neurochem.Int.. (印刷中).
• [Publications] Mishima,K.: "Molecular mechanism for α2-adrenoceptor-mediated regulation of synoviocytes proliferation."Jpn.J.Pharmacol.,. (印刷中).
• [Publications] Yonezawa,K: "Nutritional regulation of gene and protein expression."Curr.Opin.Clinic.Nutri.Metab.Care. 3. 253-254 (2000)
• [Publications] Takahashi,T.: "Carboxyl-terminal region conserved among phosphoinositide-kinase-related kinases is indispensable for mTOR function in vivo and in vitro."Genes to Cells. 5. 765-775 (2000)
• [Publications] Sato K.-I.: "Fertilization signalling and protein-tyrosine kinases."Comp.Biochem.Physiol.B.. 126. 129-148 (2000)
• [Publications] Sato K.-I.: "Regulation of Xenopus oocytes, eggs, and embryos by protein-tyrosine phosphorylation."Curr.Top.Biochem.Res.. 2. 91-102 (2000)
• [Publications] Sato K.-I.: "Tyrosine kinase-dependent activation of phospholipase Cγis required for calcium transient in Xenopus egg fertilization."Dev.Biol.. 224. 453-469 (2000)
• [Publications] Sato K.-I.: "Adaptor protein Shc undergoes translocation and mediates up-regulation of the tyrosine kinase c-Src in EGF-stimulated A431 cells."Genes to Cells. 5. 749-764 (2000)
• [Publications] Sato K.-I.: "Hydrogen peroxide induces Src family tyrosine kinase-dependent egg activation process in Xenopus eggs."Develop.Growth Differ.. 43. 55-72 (2001)
• [Publications] Tokmakov,A.: "Calcium oscillations in Xenopus egg cycling extracts."J.Cell.Biochem.. (印刷中).
• [Publications] Dong,L.Q.: "Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton."Proc.Natl.Acad.Sci.USA. 97・10. 5089-5094 (2000)
• [Publications] Nagumo,H.: "Rho kinase inhibitor HA-1077 prevents Rho-medicated myosin phosphatase inhibitation in smooth muscle cells."Am.J.Physiol.Cell Physiol.. 278. 57-65 (2000)
• [Publications] Isagawa,T.: "Dual effects of PKNα and protein kinase C on phosphorylation of tau protein by glycogen synthase kinase-3β."Biochem.Biophys.Res.Commun.. 273・1. 209-212 (2000)
• [Publications] Gao,Q.: "PKN binds and phosphorylates human Papillomavirus E6 oncoprotein."J.Biol.Chem.. 275・20. 14824-14830 (2000)
• [Publications] Takahashi,M.: "Association of immature hypo-phosphorylated protein kinase Cε with an anchoring protein CG-NAP."J.Biol.Chem.. 275・44. 34592-34596 (2000)
• [Publications] Misaki,K.: "PKN delays mitotic timing by inhibition of Cdc25C : Possible involvement of PKN in the regulation of cell division."Proc.Natl.Acad.Sci.USA. 98. 125-129 (2000)
• [Publications] Taniguchi,T.: "Phosphorylation of tau is regulated by PKN."J.Biol.Chem.. (印刷中).
• [Publications] Matsumoto,M.: "Inhibition of insulin-induced activation of Akt by a kinase-deficient mutant of the ε isozyme of protein kinase C."J.Biol.Chem.. (印刷中).
• [Publications] Takaishi,H.: "Regulation of nuclear translocation of Forkhead transcription factor AFX by protein kinase B."Proc.Natl.Acad.Sci.USA. 96. 11836-11841 (1999)
• [Publications] Aoto,M.: "A 58-kDa Shc protein is present in Xenopus eggs and is phosphorylated on tyrosine residues upon egg activation."Biochem.Biophys.Res.Commun.. 258. 265-270 (1999)
• [Publications] Sato,K.-I.: "Evidence for the involvement of a Src-related tyrosine kinase in Xenopus egg activation."Dev.Biol.. 209. 308-320 (1999)
• [Publications] Kiriyama,H.: "Identification of the catalytic subunit of cAMP-dependent protein kinase from the photosynthetic flagellate, Euglena gracilis Z."FEBS Lett.. 450. 95-100 (1999)
• [Publications] Fukami,Y.: "Peptide inhibitors of MAPK pathway ; A structure-mimetic peptide corresponding to the conserved IDA (Inter-DFG-APE) region of the kinase domain."Pharmacol.Ther.. 82. 399-407 (1999)
• [Publications] Nakazawa.M.: "Biochemical analysis of the interaction between elongation factor 1α and α/β tubulins from a ciliate, Tetrahymena pyriformis."FEBS Lett.. 435. 29-34 (1999)
• [Publications] Tokmakov,A.: "Deregulation of MAPK at low pH due to a structural rearrangement of activation segment."Biochim.Biophys.Acta. 1476. 66-74 (1999)
• [Publications] Sato K.-I.: "Fertilization signalling and protein-tyrosine kinases."Comp.Biochem.Physiol.. (印刷中).
• [Publications] Sato K.-I.: "Regulation of Xenopus oocytes, eggs, and embryos by protein-tyrosine phosphorylation."Curr.Top.Biochem.Res.. (印刷中).
• [Publications] Yamazaki,M.: "Identification of the RhoA-binding domain of phospholipase D1."J.Biol.Chem.. 274. 6035-6038 (1999)
• [Publications] Takahashi,M.: "Characterization of a novel giant scaffolding protein, CG-NAP, that anchors multiple signaling enzymes to centrosome and the Golgi apparatus."J.Biol.Chem.. 274. 17267-17274 (1999)
• [Publications] Yoshinaga,C.: "Mutational Analysis of the Regulatory Mechanism of PKN : The Regulatory Region of PKN Contains an Arachidonic Acid-Sensitive Autoinhibitory Domain."J.Biochem.. 126. 475-484 (1999)
• [Publications] Oishi,K.: "Identification and characterization of PKN β, a novel isoform of protein kinase PKN."Biochem.Biophys.Res.Comm.. 261. 808-814 (1999)
• [Publications] Shibata H.: "Interaction of PKN with a neuron-specific basic helixloop-helix transcription factor, NDRF/NeuroD2."Mol Brain Res.. 74. 126-134 (1999)
• [Publications] Tanaka,M.: "Identification of myosin II as a binding protein to the PH domain of protein kinase B."Biochem.Biophys.Res.Commun.. 255. 169-174 (1999)
• [Publications] Fujita,E.: "Akt phosphorylation site found in human caspase-9 is absent in mouse caspase-9."Biochem.Biophys.Res.Commun.. 264. 550-555 (1999)
• [Publications] Konishi,H.: "Opposing effects of protein kinase C δ and protein kinase B a on H2O2-induced apoptosis in CHO cells."Biochem.Biophys.Res.Commun.. 264. 840-846 (1999)
• [Publications] Bandyopadhyay,G.: "Effects of transiently expressed atypical (ζ λ), conventional (α,β) and novel (δ,ε) protein kinase Cisoforms on insulinstimulated translocation of epitope-tagged GLUT4 glucose transporters in rat adipocytes : specific interchangeable effects of protein kinases C-ζ and C-λ."Biochem.J.. 337. 461-470 (1999)
• [Publications] Irie,K.: "Synthesis and phorbol ester-binding studies of the individual cysteine-rich motifs of protein kinase D."Bioorg.Med.Chem.Lett.. 9. 2487-2490 (1999)
• [Publications] Tanaka,K.: "Identification of protein kinase B (PKB) as a phosphatidylinositol 3,4,5-trisphosphate binding protein in Dictyostelium discoideum."Biosci.Biotechnol.Biochem.. 63. 368-372 (1999)
• [Publications] Standaert,M.L.: "Effects of knockout of the protein kinase C βgene on glucose transport and glucose homeostasis."Endocrinology. 140. 4470-4477 (1999)
• [Publications] Yamamoto,T.: "Involvement of EF hand motifs in the Ca2+-dependent binding of the pleckstrin homology domain to phosphoinositides."Eur.J.Biochem.. 265. 481-490 (1999)
• [Publications] Ogino,C.: "Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum."J.Biochem.. 125. 263-269 (1999)
• [Publications] Konishi,H.: "Activation of protein kinase B induced by H2O2 and heat shock through distinct mechanisms dependent and independent of phosphatidylinositol 3-kinase."J.Biochem.. 126. 1136-1143 (1999)
• [Publications] Fujimoto,K.: "Identification of protein kinase C phosphorylation sites involved in phorbol ester-induced desensitization of the histamine H1 receptor."Mol.Pharmacol.. 55. 735-742 (1999)
• [Publications] Kitamura,T.: "Insulin-induced phosphorylation and activation of cyclic nucleotide phosphodiesterase 3B by the serine-threonine kinase Akt."Mol.Cell.Biol.. 19. 6286-6296 (1999)
• [Publications] Takahashi,T.: "Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells."Am.J.Physiol.. 276. H1927-H1934 (1999)
• [Publications] Nakai,M.: "Amyloid beta Protein (25-35) Phosphorylates MARCKS through Tyrosine Kinase-Activated Protein Kinase C Signaling Pathway in Microglia."J.Neurochem.. 72. 1179-1186 (1999)
• [Publications] Murakami,N.: "An association study of a polymorphism of non-erythroid α-spectrin gene with schizophrenia."American Journal of Medical Genetics.. 88. 291-293 (1999)
• [Publications] Kuroda,S.: "Biochemical characterization and expression analysis of neural thrombospondin-1-like protein, NELL1 and NELL2."Biochem.Biophys.Res.Commun.. 265. 79-86 (1999)
• [Publications] Ren,Y.: "Immunocytochemical localization of serotonin and serotonin transporter (SET) in taste buds of rat."Mol.Brain Res.. 74. 221-224 (1999)
• [Publications] Shimada,F.: "The neuroprotective agent MS-153 stimulates glutamate uptake."Eur.J.Pharmacol.. 386. 263-270 (1999)
• [Publications] Sumioka,K.: "Induction of 55 kDa PKN cleavage product by ischemia/reperfusion model in the rat retina."Invest.Opthal.Vis.Sci.. 41. 29-35 (2000)
• [Publications] Sakai,N.: "Involvement of the actin cytoskeleton in the regulation of serotonin transporter (SET) activity : possible mechanism underlying SET regulation by protein kinase C."Neurochem.Int.. 36. 567-579 (2000)
• [Publications] Oyasu,M.: "Immunocytochemical localization of a neuron-specific thrombospondin-1-like protein, NELL2 : Light and electron microscopic studies in the rat brain."Mol.Brain Res.. 76. 151-160 (2000)
• [Publications] Shirai,Y.: "Phospholipase A2 and its products are involved in the purinergic receptor-mediated translocation of protein kinase C in CHO-K1 cells."J.Cell Sci.. (印刷中).
• [Publications] Akiyama,H.: "Blood-brain barrier formation of grafted human umbilical vein endothelial cells in athymic mouse brain."Brain Res.. (印刷中).
• [Publications] Ueyama,T.: "cDNA cloning of an alternative splicing variant of protein kinase Cδ (PKCδIII), a new truncated form of PKC, in rats."Biochem.Biophys.Res.Commun.. (印刷中).
• [Publications] Parekh,D.: "mTOR controls one of two kinase oathways acting upon nPKCδ and nPKCε."J.Biol.Chem.. 274. 34758-34764 (1999)
• [Publications] Isotani,S.: "Immunopurified mammalian target of rapamycin phosphorylates and activates p70 S6 kinase α in vitro."J.Biol.Chem.. 274. 34493-34498 (1999)
• [Publications] Nanahoshi,M.: "Alpha4 protein as a common regulator of type 2A-related serine/threonine protein phosphatases."FEBS Lett.. 446. 108-112 (1999)
• [Publications] Shigemitsu,K.: "Structural requirement of leucine for activation of p70 S6 kinase."FEBS Lett.. 447. 303-306 (1999)
• [Publications] Shigemitsu,K.: "Regulation of translational effectors by amino acid and mammalian target of rapamycin signaling pathways."J.Biol.Chem.. 274. 1058-1065 (1999)
• [Publications] Nakamura, S.: "Requirement of GM2 ganglioside activator for phospholipase D activation." Proc. Natl. Acad. Sci. USA. 95. 12249-12253 (1998)
• [Publications] Tokmakov, A.A.: "Inhibition of MAPK pathway by a synthetic peptide corresponding to the activation segment of MAPK" Biochem. Biophys. Res. Commun.252. 214-219 (1998)
• [Publications] Fukami, Y.: "Peptide inhibitors of MAPK pathway; A structure-mimetic peptide corresponding to the conserved IDA(Inter-DFG-APE) region of the kinase domain." Pharmacology & Therapeutics. in press.
• [Publications] Toda, S.: "Immunohistochemical expressions of growth factors in subacute thyroiditis, and their effects on thyroid folliculogenesis and angiogenesis in collagen gel matrix culture." J. Pathol.in press.
• [Publications] Yoshida, I.: "Light-regulated gene expression of S-adenosylmethionine decarboxylase in Pharbitis nil." J. Exp. Bot.49. 617-620 (1998)
• [Publications] Frohnmeyer, H.: "Different roles for calcium and calmodulin in phytochrome- and UV-regulated expression of chalcone synthase." Plant J.13. 762-763 (1998)
• [Publications] Nakayama, M.: "Cloning and expression of the soybean chlH gene encoding a subunit of Mg-chelatase and localization of the Mg^<2+> concentration-dependent chlH protein within the chloroplast." Plant Cell Physiol.39. 275-284 (1998)
• [Publications] Yoshida, I.: "Blue-and red-light regulation and circadian control of gene expression of s-adenosylmethionine decarboxylase in Pharbitis nil." J. Exp. Bot.in press.
• [Publications] Nishio, N.: "Possible role of alterration in pH, but not in ionic strength, in modulation of Ca^<2+>-dependent and diacylglycerol-induced association of protein kinase C-α with plasma membrane." Biochem. Mol. Biol. Int.46. 725-731 (1998)
• [Publications] Kim, H-S.: "Effect of α-tocopherol status on α-tocopherol transfer protein expression and its messenger RNA level in rat liver." Free Rad. Res.28. 87-92 (1998)
• [Publications] Yamaji, A.: "Lysenin, a novel sphingomyelin-specific binding protein." J. Biol. Chem.273. 5300-5306 (1998)
• [Publications] Wei, Y.: "Structure of a microbial homologue of mammalian platelet-activating factor acetylhydrolases:streptomyces exfoliatus lipase at 1.9A resolution." Structure. 6. 511-519 (1998)
• [Publications] Nomura, T.: "Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide β-1, 4-galactosyltransferase from rat brain." J. Biol. Chem.273. 13570-13577 (1998)
• [Publications] Manya, H.: "Switching of platelet-activating factor acetylhydrolase catalytic subunits in developing rat brain." J. Biol. Chem.273. 18567-18592 (1998)
• [Publications] Arita, M.: "Binding of α-tocopherylquinone, an oxidized form of α-tocopherol, to glutathione-S-transferase in the liver cytosol." FEBS Lett.436. 424-426 (1998)
• [Publications] Majima, M.: "Significant roles of inducible cyclooxygenase (COX)-2 in angiogenesis in rat sponge implants." Jpn. J. Pharmacol.75. 105-114 (1998)
• [Publications] Bisogno, T.: "Biosynthesis and degradation of bioactive fatty acid amides in human breast cancer and rat pheochromocytoma cells: implications for cell proliferation and differentiation." Eur. J. Biochem.254. 634-642 (1998)
• [Publications] Goparaju, SK.: "Anandamide amidohydrolase reacting with 2-〓 arachidonoylglycerol, another cannabinoid receptor ligand." FEBS Lett.422. 69-73 (1998)
• [Publications] Nakamura, M.: "Platelet-type arachidonate 12-lipoxygenase in mouse gastrointestinal tract." Acta Histochem. Cytochem.31. 71-75 (1998)
• [Publications] Yamamoto, S.: "Transcriptional regulation of fatty acid cyclooxygenases-1 and -2." Int. J. Tissue React.20. 17-22 (1998)
• [Publications] Nakamura, M.: "Involvement of tyrosine phosphorylation in inhibition of FMLP-induced PLD activation by N-acetyl-L-cysteine in differentiated HL-60 cells." J. Leuk. Biol.63. 781-789 (1998)
• [Publications] Kanoh, H.: "Molecular cloning of a gene encoding phospholipase D fromthe pathogenic and dimorphic fungus, Candida albicans." Biochem. Biophys. Acta. 1398. 359-364 (1998)
• [Publications] Leidich, S.D.: "Cloning and disruption of the Candida albicans phospholipase B gene, CaPLB1." J. Biol. Chem. 273. 26078-26086 (1998)
• [Publications] Wang, S.: "Molecular cloning and cell cycle-dependent expression of a novel NIMA-related protein kinase (TpNrK) in Tetrahymena cells." Biochem. J.334. 197-203 (1998)
• [Publications] Banno, Y.: "Evidence for the presence of multiple forms of sphingosine kinase in human platelets." Biochem. J.335. 301-304 (1998)
• [Publications] Mizutani, T.: "Changes in the expression of protein kinase C (PKC), phospholipases C (PLC) and D (PLD) isoforms in spleen,D brain and kidney of the aged rat: RT-PCR and Western blot analysis." Arch. Gerontol. Geriatr.105. 151-172 (1998)
• [Publications] Hayakawa, K.: "Decreased phospholipase D activities in ceramide-induced apoptosis of RBL-2H3 mast cells." Biomed. Res.19. 159-170 (1998)
• [Publications] Okamoto, H.: "Studies on blood compatibility of terpolymers composed of methylmethacrylate methoxypolyethyleneglycol methacrylate and dimethylsiloxane methacrylate." J. Biomater. Sci. Polymer Edn.9. 934-959 (1998)
• [Publications] Ito, Y.: "Possible involvement of mitogen-activated protein kinase in phospholipase D activation induced by H_2O_2, but not by carbachol, in rat pheochromocytoma PC12 cells." J. Neurochem.71. 2278-2285 (1998)
• [Publications] Nomura, T.: "Identification of GTP cyclohydrolase I activity in the ciliated protozoa, Tetrahymena pyriformis." Comp. Biochem. Physiol.B120. 753-760 (1998)
• [Publications] Nagata, K.: "Expression of GTP-binding proteins and protein kinase C isozymes in platelet-like particles derived from megakaryoblastic leukemia cells (MEG-O1)." Platelets. in press.
• [Publications] Kasai, T.: "Increased activity of oleate-dependent type phospholipase D during actinomycin D-induced apoptosis in Jurkat T cells." J. Immunol.in press.
• [Publications] Takenaka, K.: "Analysis for phospholipase C gene in patients with subarachnoid hemorrhage due to ruptured intracranial saccular aneurysm." Neurol. Res.in press.
• [Publications] Ohguchi, K.: "Phospholipase D development during differentiation of HL60 cells." Biochim. Biophys. Acta. in press.
• [Publications] Sugiyama, Y.: "Molecular cloning of a second phospholipase B gene, caPLB2 from Candida albicans." Med. Mycol.in press.
• [Publications] Nakashima, S.: "Phospholipase D development during differentiation of HL60 cells." Chem. Phys. Lipid, Possible role of phospholipase D (PLD) in cellular differentiation and apoptosis.in press.
• [Publications] Kobayasi, S.: "Catalytic asymmetric syntheses of antifungal sphingofungins and their biological activity as potent inhibitors of serine palmitoyltransferase (SPT)." J. Am. Chem. Soc.120. 908-919 (1998)
• [Publications] Saito, K.: "Genetic evidence that phosphatidylserine synthase II catalyzes the conversion of phosphatidylethanolamine to phosphatidylseine in Chinese hamster ovary cells." J. Biol. Chem.273. 17199-17205 (1998)
• [Publications] Shiozaki, M.: "Revised structure and synthesis of flavolipin." Tetrahedron. 54. 11861-11876 (1998)
• [Publications] Suzuki, T.: "Reduction of caveolin-1 expression in tumoreigenic human cell hybrids." J. Biochem.124. 383-388 (1998)
• [Publications] Ou, Z.L.: "Competitive reverse transcription-polymerase chain reaction for determination of rat CC and C chemokine mRNAs." Anal. Biochem.261. 227-229 (1998)
• [Publications] Hanada, K.: "Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the lcb1 protein with the lcb2 protein for serine palmitoyltransferase." J. Biol. Chem.273. 33787-33794 (1998)
• [Publications] Kawasaki, K.: "Isolation of a Chinese hamster ovary (CHO) cDNA encoding phosphatidylglycerophosphate (PGP) synthase, expression of which corrects the mitochondrial abnormalities of a PGP synthase-defective mutant of CHO-K1 cells." J. Biol. Chem.in press.
• [Publications] Yamamoto, H.: "Involvement of phospholipase D in lipopolysaccharide-induced nuclear factor kB activation in mouse macriohage-like J774.1 cells." J. Immunol.in press.
• [Publications] Fukasawa, M.: "Genetic evidence for ATP-dependent ER-to-Golgi trafficking of ceramide for sphingomyelin synthesis in CHO cells." J. Cell Biol.in press.
• [Publications] Uchida, K.: "Induction of apoptosis by phosphatidylserine." J. Biochem.123. 1073-1078 (1998)
• [Publications] Boronenkov, I.V.: "Phosphoinositide signaling pathways in nuclei are associated with nuclear speckles containing pre-mRNA processing factors." Mol. Biol. Cell. 9. 3547-3560 (1998)
• [Publications] Qin, S.: "Differential regulation of oxidative and osmotic stress-induced Syk activation by both autophosphorylation and SH2 domains." Biochemistry. 37. 5481-5486 (1998)
• [Publications] Oishi, I.: "A novel Drosophila nuclear protein serine/threonin kinase expressed in the germ line during its establishment." Mech. Dev.71. 49-63 (1998)
• [Publications] Yoneya, H.: "Antibodies directed against ZAP-70 cross react with a 66 kDa tyrosine kinase in the rat brain." Biochem. Biophys. Res. Commun.245. 140-143 (1998)
• [Publications] Liu, Z-J.: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." Mol. Cell. Biol.18. 3445-3454 (1998)
• [Publications] Qin, S.: "A deficiency in Syk enhances ceramide-induced apoptosis in DT40 lymphoma B cells." FEBS Lett.427. 139-143 (1998)
• [Publications] Rezaul, K.: "Invlvement of ractive oxygen intermediates in lectin-induced protein-tyrosine phosphorylation of Syk in THP-1 cells." Biochem. Biophys. Res. Commun.246. 863-867 (1998)
• [Publications] Tohyama, Y.: "Outside-in signaling of soluble and solid-phase fibrinogen through integrin αIIBβ3 is different and cooperative with each other in a megakaryoblastic leukemia cell, CMK." Blood. 92. 1277-1286 (1998)
• [Publications] Liu, Z-J.: "Functional cooperation of cyclin C and c-Myc in mediating homotypic cell adhesion via very late antigen-4 activation and vascular cell adnesion molecule-1 induction." Blood. 92. 1-13 (1998)
• [Publications] Shimohama, S.: "Differential expression of rat brain phospholipase C isozymes in development and aging." Biochem. Biophys. Res. Commun.243. 210-216 (1998)
• [Publications] Shimohama, S.: "Phospholipase C isozymes in the human brain and their changes in Alzheimer's disease." Neuroscience. 82. 999-1007 (1998)
• [Publications] He, H.: "Role of phosphatidylinositol 4,5-bisphosphate in Ras/Rac-induced disruption of the cortactin-actomyosin II complex and malignant Transformation." Mol. Cell. Biol.18. 3829-3837 (1998)
• [Publications] Suzuki, T.: "Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri H." EMBO J.15. 2767-2776 (1998)
• [Publications] Itoh, T.: "A novel phosphatidylinositol-5-phosphate 4-kinase (phosphatidylinositol-phosphate kinase IIγ) is phosphorylated in the endoplasmic reticulum in response to mitogenic signals." J. Biol. Chem.273. 20292-20299 (1998)
• [Publications] Suetsugu, S.: "The essential role of profilin in the assembly of actin for microspike formation." EMBO J.17. 6516-6526 (1998)
• [Publications] Miki, H.: "WAVE, a novel WASP-family protein involved in actin-polymerization induced by Rac." EMBO J.in press.
• [Publications] Nagano, K.: "A novel phospholipase Cδ4 splice variants as a negative regulator of PLC." J. Biol. Chem.in press.
• [Publications] Hayashi, T.: "The AMPA receptor interacts with and signals through the protein tyrosine kinase Lyn." Nature. in press.
• [Publications] Umemori, H.: "Stimulation of myelin basic protein gene transcription by Fyn tyrosine kinase for myelination." J. Neurosci.in press.
• [Publications] Tezuka, T.: "PSD-95 promotes Fyn-mediated tyrosine phosphorylation of the N-methyl-D-aspartate receptor subunit NR2A." Proc. Natl. Acad. Sci. USA. in press.
• [Publications] Sano, Y.: "CBP activates the intramolecular inhibition of ATF-2 function." J. Biol. Chem.in press.
• [Publications] Kohno, T.: "Homozygous deletion and frequent allelic loss of the 21q11.1-q21.1 region including the ANA gene in human lung carcinema." Gene, Chromosomes & Cancer. 21. 236-243 (1998)
• [Publications] Tsuda, M.: "Integrin-mediated tyrosine phosphorylation of SHPS-1 and its association with SHP-2." J. Biol. Chem.273. 13323-13229 (1998)
• [Publications] Takeda, H.: "Lysophosphatidic acid-induced association of SHP-2 with SHPS-1: roles of RHO, FAK, and a SRC family kinase." Oncogene. 16. 3019-3027 (1998)
• [Publications] Kametani, Y.: "Ex vivo evidence for asymmetric tyrosine phosphorylation of ZAP-70 on double-positive thymocytes in the positive selection process." Int. Immunol.10. 1203-1210 (1998)
• [Publications] Suzuki, T.: "Essential roles of Lyn in fibronectin-meiated filamentous-actin assembly and cell motility in mast cells." J. Immunol.161. 3964-3701 (1998)
• [Publications] Kimura, K.: "Regulation of the association of adducin with actin filaments by Rho-kinase and myosin phosphatase." J. Biol. Chem.273. 5542-5548 (1998)
• [Publications] Amano, M.: "Myosin II activation promotes neurite retraction during the action of Rho and Rho-kinase." Genes Cells. 3. 177-188 (1998)
• [Publications] Kuroda, S.: "Involvement of IQGAP1, a target of the small GTPases Cdc42 and Rac1, in the regulation of E-cadherin-mediated cell-cell adhesion." Science. 281. 832-835 (1998)
• [Publications] Ihara, K.: "Crystal structure of human RhoA in a dominantly active formcomplexed with a GTP analogue." J. Biol. Chem.273. 9656-9666 (1998)
• [Publications] Fujioka, M.: "A new isoform of human myosin phosphatase targeting/regulatory subunit (MYPT12): cDNA cloning, tissue expression, and cromosomal mapping." Genomics. 49. 59-68 (1998)
• [Publications] Goto, H.: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." J. Biol. Chem.273. 11728-11736 (1998)
• [Publications] Amano, M.: "Regulation of cytoskeleton and cell adhesions by the small GTPase Rho and its targets." Trend Cardio. Med.8. 162-168 (1998)
• [Publications] Hashimoto, R.: "Domain- and site-specific phosphorylation of bovine NF-L by Rho-associated kinase." Biochem. Biophys. Res. Commun.245. 407-411 (1998)
• [Publications] Taya, S.: "The Ras target AF-6 is a substrate of the Fam deubiquitinating enzyme." J. Cell Biol.142. 1053-1062 (1998)
• [Publications] Kaibuchi, K.: "Regulation of cytoskeletons and cell adhesions by the Rho family GTPases." Annu. Rev. Biochem.in press.
• [Publications] Torii, M.: "Transcription factors Mash-1 and Prox-1 delineate early steps in differentiation of neural stem cells in the developing central nervous system." Development.in press.
• [Publications] Kosako, H.: "Specific accumulation of Rho-associated kinase at the cleavage furrow during cytokinesis: cleavage furrow-specific phosphorylation of intermediate filaments." Oncogene. in press.
• [Publications] Oshiro, N.: "Phosphorylation of moesin by Rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures." J. Biol. Chem.273. 34663-34666 (1998)
• [Publications] Yasui, Y.: "Roles of Rho-associated kinase in cytokinesis; mutations in Rho-associated kinase phosphorylation sites impair cytokinetic segregation of glial cells." J. Cell Biol.in press.
• [Publications] Tatematsu, K.: "Transcriptional activity of RBCK1 protein (RBCC protein interacting with PKC 1): requirement of RING-finger and B-Box motifs and regulation by protein kinases." Biochem. Biophys. Res. Commun.247. 392-396 (1998)
• [Publications] Takeuchi, H.: "Phosphotyrosine binding domain of insulin receptor substrate-1 binds inositol compounds." Biochem. J.334. 211-218 (1998)
• [Publications] Yokozeki, T.: "Phatidic acid-dependent phosphorylation of a 29-kDa protein by protein kinase Cα in bovine brain cytosol." J. Neurochem.71. 410-417 (1998)
• [Publications] Kitamura, T.: "Requirement for activation of the serine-threonine kinase Akt (protein kinase B) in insulin stimulation of protein synthesis but not of glucose transport." Mol. Cell. Biol.18. 3708-3717 (1998)
• [Publications] Kuroda, S.: "Requirement of the serine/threonine kinase Akt for heat treatment-induced activation of p70 S6 kinase." Biochem. Biophys. Res. Commun.249. 781-785 (1998)
• [Publications] Irie, K.: "Studies of the molecular basis for protein kinase C (PKC) isozyme selective binding: the synthesis, folding, and phorbol ester binding of the cysteine-rich domains of all PKC isozymes." J. Am. Chem. Soc.120. 9159-9167 (1998)
• [Publications] Tokunaga, C.: "Molecular cloning and characterization of RBCK2, a splicing variant of a RBCC family protein, RBCK1." FEBS Lett.435. 11-15 (1998)
• [Publications] Standaert, M.: "Comparative effects of GTPγS and insulin on the activation of Rho, phosphatidylinositol 3-kinase, and protein kinase N in rat adipocytes-relationship to glucose transport." J. Biol. Chem.273. 7470-7477 (1998)
• [Publications] Takahashi, M.: "Cleavage of PKN by caspase-3 or related protease during apoptosis." Proc. Natl. Acad. Sci. USA. 95. 11566-11571 (1998)
• [Publications] Kawamata, T.: "A protein kinase, PKN, accumulates in Alzheimer neurofibrillary tangles and associated endoplasmic reticulum-derived vesicles and phosphorylates Tau protein." J. Neurosci.18. 7402-7410 (1998)
• [Publications] Hashimoto, T.: "Localization of PKN mRNA in the rat brain." Mol. Brain Res.59. 143-153 (1998)
• [Publications] Kitagawa, M.: "The role of PKN in the regulation of αB-crystallin expression via heat shock transcription factor 1." Biochem. Biophys. Res. Commun.in press.
• [Publications] Izumi, Y.: "A metalloprotease-disintegrin, MDC9/Meltrin-γ/ADAM9, and PKCδ are involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor." EMBO J.24. 7260-7272 (1998)
• [Publications] Suzuki, H.: "Recent Advances in Prostaglandin, Thromboxane, and Leukotriene Research" Sinzinger, H., Samuelsson, B., Vane, J.R., Paoletti, R., Ramwell, P., Wong, P.Y.-K., eds., Plenum Press, New York, 4 (1998)
• [Publications] Ueda, N.: "Eicosanoids and Related Compounds in Plants and Animals" Rowley, A.F., Kuhn, H., Schewe, T., eds., Portland Press, 21 (1998)
• [Publications] Yonehara, S.: "Apoptosis: Its Roles and Mechanism" Yamada, T. and Hashimoto, Y., eds., Business Center for Academic Societies Japan, Tokyo, 15 (1998)
• [Publications] Minami, Y.: "Redox Regulation of Cell Singaling and its Clinical Applications" Marcel Dekker Inc., NY, USA in press,
• [Publications] Kanaide, H.: "Methods in Molecular Biology Series, Calcium Signaling Protocols" Lambert, D., ed., Human Press Inc., U.S.A. in press,
• [Publications] Konishi,H.: "Activation of protein kinase C by tyrosine phosphorylation in response to H_2O_2" Proc.Natl.Acad.Sci.USA. 94. 11233-11237 (1997)
• [Publications] Hurley,J.H.: "Taxonomy and function of C1 protein kinase C homology domains." Protein Sci.6. 477-480 (1997)
• [Publications] Hullin-Matsuda,F.: "Ca^<2+>-independent cytosolic phospholipase A in HL60 cells differentiating to granulocytes." FEBS Lett.419. 117-120 (1997)
• [Publications] Kadotani,M.: "Characterization of tyrosine-phosphorylated δ isoform of protein kinase C isolated from Chinese hamster ovary cells." J.Biochem.121. 1047-1053 (1997)
• [Publications] Yoshida,H.: "Isolation and properties of a novel phospholipase A from rat brain that hydrolyses fatty acids at sn-1 and sn-2 positions." Ann.Clin.Biochem.(in press).
• [Publications] Akisue,T.: "Purification of a heat-stable activator protein for ADP-ribosylation factor-dependent phspholipase D." FEBS Lett.(in press).
• [Publications] Sato,K.-I.: "Phosphatidylinositol 4,5-bisphosphate stimulates phosphorylation of the adaptor protein She by c-Src." FEBS Lett.410. 136-140 (1997)
• [Publications] Tokmakov,A.A.: "Phosphorylation-sensitive secondary structure in a synthetic peptide corresponding to the activation loop of MAP kinase." Biochem.Biophys.Res.Commun.236. 243-247 (1997)
• [Publications] Sato,K.-I.: "Tyrosine residues 239 and 240 of She are phosphatidylinositol 4,5-bisphosphate-dependent phosphorylation sites by c-Sre." Biochem.Biophys.Res.Commun.240. 399-404 (1997)
• [Publications] Sato,K.-I.: "Involvement of protein-tyrosine phosphorylation and dephosphorylation in sperm-induced Xenopus egg activation." FEBS Lett.(in press).
• [Publications] Fukami,Y.: "Activation and translocation of a Sre-family protein-tyrosine kinase in fertilized Xenopus eggs." J.Reprd.Dev.43. 113-114 (1997)
• [Publications] Sato,K.-I.: "Possible involvement of protein-tyrosine phosphorylation and dephosphorylation in Xenopus egg fertilization." Zygote. (in press).
• [Publications] Fujitani,N.: "Immunocytochemical detection of enhanced expression of c-myc protein in the heart of cardiomyopathic hamster." Mol.Cell.Biochem.169. 73-78 (1997)
• [Publications] Toda,S.: "Transforming growth Factor-β1 induces a mesenchyme-like cell shape wiothout epithelial polarization in thyrocytes and inhibits thyroid folliculogenesis in collagen gel culture." Endocrinology. 138. 5561-5575 (1997)
• [Publications] Neuhaus,G.: "Phytochrome-regulated repression of gene expression requires calcium and cGMP." EMBOJ.16. 2554-2564 (1997)
• [Publications] Yamagata,H.: "Molecular cloning and characterization of a cDNA encoding early light-inducible protein from soybean(Glycine max L.)." Biosci.Biotech.Biochem.61. 2143-2144 (1997)
• [Publications] Yamagata,H.: "Molecular cloning of two cDNAs encoding small GTP-binding proteins from dark-adapted soybean suspension-cultured cells." Plant Physiol.115. 1730 (1997)
• [Publications] Yamagata,H.: "Molecular cloning and characterization of a cDNA encoding asparagine synthetase from soybean(Glycine max L.)cell cultures." Biosci.Biotech.Biochem.62. 148-150 (1998)
• [Publications] Suga,K.: "Down-regulation of protein kinase C-α detected in human colorectal cancer." Biochem.Mol.Biol.Int.44. 523-528 (1998)
• [Publications] Ho,Y.S.: "Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer." Nature. 385. 89-93 (1997)
• [Publications] Sato,T.: "Serine phospholipid-specific phospholipase A that is secreted from activated platelets." J.Biol.Chem.272. 2192-2198 (1997)
• [Publications] Murakami,M.: "Regualtory functions of phospholipase A2." Crit.Rev.Immunol.17. 225-283 (1997)
• [Publications] Adachi,H.: "Differential tissue distribution of the β-and γ-subunits of human cytosolic platelet-activating factor acetylhydrolase(isoform I)." Biochem.Biophys.Res.Commun.233. 10-13 (1997)
• [Publications] Emoto,K.: "Exposure of phosphatidylethanolamine on the surface of apoptic cells." Exp.Cell Res.232. 430-434 (1997)
• [Publications] Hosomi,A.: "Affinity for α-tocopherol transfer protein as a determinant of the biological activities of vitamin Eanalogs." FEBS Lett.409. 105-108 (1997)
• [Publications] Adachi,H.: "cDNA cloning and expression of chicken aminopeptidase H,possessing endopeptidase as well as aminopeptodase activity." Eur.J.Biochem.245. 283-288 (1997)
• [Publications] Yokota,T.: "Friedreich-like ataxia with retinitis pigmentosa caused by the His 101Gln mutation of the α-tocopherol transfer protein gene." Ann.Neurol.41. 826-837 (1997)
• [Publications] Murakami,M.: "Regulatory functions of phospholipase A2" Crit.Rev.Immunol.17. 225-283 (1997)
• [Publications] Adachi,T.: "PAF analogues capable of inhibiting PAF acetylhydrolase activity suppress migration of isolated rat cerebellar granule cells." Neurosci.Lett.235. 133-136 (1997)
• [Publications] Arita,M.: "α-Tocopherol transfer protein stimulates the secretion of α-tocopherol from a cultured liver cell line through a brefeldin A-insensitive pathway." Proc.Natl.Acad.Sci.USA. 94. 12437-12441 (1997)
• [Publications] Nakano,T.: "Cell adhesion to phosphatidylserine mediated by a product of growth arrest-specific gene 6." J.Biol.Chem.272. 29411-29414 (1997)
• [Publications] Matsuzawa,A.: "Protection against oxidative stress-induced cell death by intracellular platelet-activating factor-acetylhydrolase II." J.Biol.Chem.272. 32315-32320 (1997)
• [Publications] Adachi,H.: "Expression cloning of a novel scavenger receptor from human endothelial cells." J.Biol.Chem.272. 31217-31220 (1997)
• [Publications] Watanabe,M.: "Molecular cloning of cDNAs encoding α1,α2,and β subunits of rat brain platelet-activating factor acetylhydrolase." Biochim.Biophys.Acta. 1401. 73-79 (1997)
• [Publications] Tomo,K.: "Qualitative platelet 12-lipoxygenase abnormality in a patient with essential thrombocythemia." Thromb.Haemost.77. 294-297 (1997)
• [Publications] Ueda,N.: "Induction of cyclooxygenase-1 in a human megakaryoblastic cell line(CMK)differentiated by phorbol ester." Biochim.Biophys.Acta. 1344. 103-110 (1997)
• [Publications] Nakamura,M.: "Subcellular localization of arachidonate 12-lipoxygenase and morphological effect of its overexpression on murine keratinocytes." Cell Tissue Res.288. 327-334 (1997)
• [Publications] Suzuki,H.: "A selective inhibitor of arachidonate 5-lipoxygenase scavenging peroxide activator." Biochem.Pharmacol.54. 529-532 (1997)
• [Publications] Liu,Y.-W.: "Transcriptional activation of human 12-lipoxygenase gene promoter is mediated through Spl consensus sites in A43l cells." Biochem.J.324. 133-140 (1997)
• [Publications] Liu,Y.-W.: "Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells." Biochim.Biophys.Acta. 1344. 38-46 (1997)
• [Publications] Chen,B.-K.: "Overexpression of Ha-ras enhances the transcription of human arachidonate 12-lipoxygenase promoter in A431 cells." Biochim.Biophys.Acta. 1344. 270-277 (1997)
• [Publications] Mahmud,I.: "Prostaglandin D Synthase in human megakaryoblastic cells." J.Biol.Chem.272. 28263-28266 (1997)
• [Publications] Kawajiri,H.: "Diurnal change of arachidonate 12-lipoxygenase in rat pineal gland." Biochem.Biophys.Res.Commun.238. 229-233 (1997)
• [Publications] Kurahashi,Y.: "Reversible hydrolysis and synthesis of anandamide demonstrated by recombinant rat fatty-acid amide hydrolase." Biochem.Biophys.Res.Commun.237. 512-515 (1997)
• [Publications] Katayama,K.: "Distribution of anandamide amidohydrolase in rat tissues with special reference to amall intestine." Biochim.Biophys.Acta. 1347. 212-218 (1997)
• [Publications] De Petrocellis,L.: "Novel inhibitors of brain,neuronal,and basophilic anandamide amidohydrolase." Biochem.Biophys.Res.Commun.231. 82-88 (1997)
• [Publications] Goparaju,S.K.: "Anandamide amidohydrolase reacting with 2-arachidonoylglycerol,another cannabinoid receptor ligand." FEBS Lett.422. 69-73 (1998)
• [Publications] Ito,Y.: "Implication of Ca^<2+>-dependent protein tyrosine phosphorylation in carbachol-induced phospholipase D activation in rat pheochromocytoma PC12 cells." J.Neurochem.68. 419-425 (1997)
• [Publications] Ohguchi,K.: "Increased activity of small GTP-binding protein-dependent phospholipase D during differentiation in human promyelocytic leukemic HL60 cells." J.Biol.Chem.272. 1990-1996 (1997)
• [Publications] Nagata,K.: "Differential expression of low Mr GTP-binding proteins in human megakaryoblastic leukemia cell line,MEG-01,and thein possible involvement in the differentiation process." Thromb.Haemost.77. 368-375 (1997)
• [Publications] Hammond,S.M.: "Characterization of two alternately spliced forms of phospholipase D1." J.Biol.Chem.272. 3860-3868 (1997)
• [Publications] Banno,Y.: "Nuclear ADP-ribosylation factor(ARF)-and oleate-dependent phospholipase D(PLD)in rat liver cells." J.Biol.Chem.272. 5208-5213 (1997)
• [Publications] Nakashima,T.: "Purification of a factor from CSF in patient after SAH which induces the cytosolic free calcium elevation in vascular smooth muscle cells." Neurol.Res.19. 51-56 (1997)
• [Publications] Yoshimura,S.: "Differential expression of Rho family GTP-binding proteins and protein kinase C isozymes during C6 glial cell differentiation." Mol.Brain Res.45. 90-98 (1997)
• [Publications] Sakai,H.: "Identification of differentially expressed mRNAs during rat C6 glial cell differentiation by mRNA fingerprinting using arbitrarily primed PCR(RAP)." Neurosci.Lett.229. 93-96 (1997)
• [Publications] Yoshimura,Y.: "Changes in the activity and mRNA levels of phospholipase D during ceramide-induced apoptosis in rat glial cells." J.Neurochem.69. 713-720 (1997)
• [Publications] Ito,Y.: "Hydrogen peroxide-induced phospholipase D activation in rat pheochromocytoma PC12 cells:Possible involvement of Ca^<2+>-dependent protein tyrosine kinase." J.Neurochem.69. 729-736 (1997)
• [Publications] Ohguchi,K.: "Tyrosine phosphorylation of 100-115 kDa proteins by phosphatidic acid generated via phospholipase D activation in HL60 granulocytes." Biochim.Biophys.Acta. 1346. 301-304 (1997)
• [Publications] Takemura,O.S.: "Inhibition of N-formylmethionyl-leucyl-pyenylalanine-stimulated tyrosine phosphorylation and phospholipase D activation by quercetin in rabbit neutrophils." Biochem.Pharmacol.53. 1503-1510 (1997)
• [Publications] Kato,Y.: "Involvement of rho family proteins in prostaglandin F_<2α>-induced phospholipase D activation in the osteoblast-like cell line MC3T3-E1." Prostaglandins. 54. 475-492 (1997)
• [Publications] Nakamura,M.: "Effect of wortmannin and 2-(4-morpholinyl)-8-phenyl4H-1-benzopyran-4-one(LY294002)on N-formyl-methionyl-leucyl-phenylalanine-induced phospholipase D activation in differentiated HL60 cells." Biochem.Pharmacol.53. 1929-1936 (1997)
• [Publications] Adachi,T.: "Possible involvement of pertussis toxin-sensitive G protein in hepatocyte growth factor-induced signal transduction in cultured rat hepatocytes:pertussis toxin treatment inhibits activation of phospholipid signaling,calcium oscillation,and mitogen-activated protein kinase." Hepatology. 26. 295-300 (1997)
• [Publications] Liu,B.: "Prolonged activation of phospholipase D in Chinese hamster ovary cells expressing PAF receptor lacking cytoplasmic C-terminal tail." Biochem.J.327. 239-244 (1997)
• [Publications] Nakashima,S.: "Molecular cloning and chromosome mapping of rat phospholipase D genes rPldla,rPldlb and rPld2." Cytogenet.Cell Genet.(in press).
• [Publications] Nakashima,S.: "Increased mRNA expression of phospholipase D(PLD)isozymes during granulocytic differentiation of HL60 cells." Biochim.Biophys.Acta. (in press).
• [Publications] Sakai,H.: "mRNA fingerprinting analysis using arbitrarily primed PCR(RAP)of genes expressed during rat C6 glioma cell differentiation." Brain Tumor Pathol.(in press).
• [Publications] Dohjima,T.: "Preferential down-regulation of phospholipase C-β1(PLC-β1)during the early stage of differentiation in MC3T3-E1 osteoblast cell." Biomed.Res.(in press).
• [Publications] Sakai,H.: "Molecular cloning of a cDNA encodding a serine protease homologous to complement Cls precursor from rat C6 glial cells and its expression during glial differentiation." Gene.(in press).
• [Publications] Iwasaki-Bessho,Y.: "Decreased phospholipase D(PLD)activity in ceramide-induced apoptosis of human keratinocyte cell line HaCaT." J.Invest.Dermatol.(in press).
• [Publications] Ojio,K.: "Role of Rho family GTP-binding proteins in IgE receptor-mediated phospholipase D activation in mast cells." Biomed.Res.(in press).
• [Publications] Banno,Y.: "Stimulation by G protein βγ subunits of phospholipase Cβ isoforms in human platelets." Thromb.Haemost.(in press).
• [Publications] Yoshimura,S.: "Ceramide formation leads to caspase-3 activation during hypoxic PC12 cell death:Inhibitory effects of Bcl-2 on ceramide formation and caspase-3 activation." J.Biol.Chem.(in press).
• [Publications] Fukasawa,M.: "Involvement of heparan sulfate proteoglycans in the bioding step for phagocytosis of latex beads by Chinese hamster ovary cells." Exp.Cell Res.230. 154-162 (1997)
• [Publications] Shinoura,N.: "Brain tumors:detection with ^<11>C-choline PET^1." Radiology. 202. 497-503 (1997)
• [Publications] Muro,M.: "Role of CD14 Molecules in internalization of Actinobacillus actinomycetemcomitans by macrophages and subsequent induction of apoptosis." Infect.Immun.65. 1147-1151 (1997)
• [Publications] Shiozaki,M.: "The first syntheses of GLA-60 positional isomers and their biological activities." Bull.Chem.Soc.Jpn.70. 1149-1161 (1997)
• [Publications] Yamamoto,H.: "Involvement of diacylglycerol production in activation of nuclear factor-κB by a CD14-mediated lipopolysaccharide stimulus." Biochem.J.325. 223-228 (1997)
• [Publications] Nishijima,M.: "Mammalian cell mutants of membrane phospholipid biogenesis." Trends Cell Biol.7. 324-329 (1997)
• [Publications] Kuge,O.: "Cloning of a Chinese hamster ovary(CHO)cDNA encoding phosphatidylserine synthase PSS)II,overexpression of which suppresses the phosphatidylserine biosynthetic defect of a PSS I-lacking mutant of CHO-KI cells." J.Biol.Chem.272. 19133-19139 (1997)
• [Publications] Kuge,O.: "Phosphatidylserine synthase I and II of mammalisan cells." Biochim.Biophys.Acta. 1348. 151-156 (1997)
• [Publications] Yamamoto,H.: "Inhibitory effect of Curcumin on mammalian phospholipase D activity." FEBS Lett.417. 196-198 (1997)
• [Publications] Hanada,K.: "A mammalian homolog of the yeast LCBl encodes a component of serine palmitoyltransterase,the enzyme catalyzing the first step in sphingolipid synthesis." J.Biol.Chem.272. 32108-32114 (1997)
• [Publications] Shiozaki,M.: "Syntheses of 2,6-anhydro-3-deoxy-5-0-phosphono-3-tetradecanamido-4-0-[(R)-3-(tetradecanoyloxy)tetradecanoyl]-D-glycero-D-ido-heptonic Acid,its dimeric analogue,and relaed compounds." Tetrahedron. 53. 16041-16060 (1997)
• [Publications] Kuge,O.: "Control of phosphatidylserine biosynthesis through phosphatidylserine-mediated inhibition of phosphatidylserine synthase I in Chinese hamster ovary cells." Pro.Natl.Acad.Sci.USA. (in press).
• [Publications] Qin,S.: "Up-regulation of Syk activity during HL60 cell differentiation into granulocyte but not into monocyte/macrophage-lineage." Biochem.Biophys.Res.Commun.236. 697-701 (1997)
• [Publications] Oishi,I.: "A novel Drosophila receptor tyrosine kinase expressed specifically in the nervous system." J.Biol.Chem.272. 11916-11923 (1997)
• [Publications] Qin,S.: "Distinctive functions of Syk of and Lyn in mediating osmotic stress and ultraviolet C irradiation-induced apoptosis in chicken B cells." J.Biol.Chem.272. 17994-17999 (1997)
• [Publications] Rezaul,K.: "Platelet-activating factor stimulates calcium-dependent activation of protein-tyrosine kinase Syk in human B lymphocytes." Biochem.Biophys.Res.Commun.239. 23-27 (1997)
• [Publications] Sada,K.: "Relocation of Syk protein-tyrosine kinase to the actin filament network and subsequent association with Fak." Eur.J.Biochem.248. 827-833 (1997)
• [Publications] Tubokawa,M.: "Interleukin-3 activates Syk in a human myeloblastic leukemia cell line,AML193." Eur.J.Biochem.249. 792-796 (1997)
• [Publications] Miki,H.: "A tyrosine kinase signalling regulates WASP function,which is essential for megakaryocyte differentiation." Cell Grwoth Differ.8. 195-202 (1997)
• [Publications] Takenawa,T.: "Signaling through Grb2/Ash-control of the Ras pathway and the cytoskeleton." Cur.Topic Microbiol.Immunol.228. 325-342 (1997)
• [Publications] Houssa,B.: "Cloning of a novel human diacylglycerol kinase containing three cystein-rich domains,a proline-rich region and a pleckstrin homology domain with overlapping ras-associating domain." J.Biol.Chem.272. 10422-10428 (1997)
• [Publications] Sakisaka,T.: "Phosphatidylinositol 4,5-bisphosphate phosphatase regulates the rearrangement of actin filaments." Mol.Cell.Biol.17. 3641-3649 (1997)
• [Publications] Matuoka,K.: "A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motif." Biochem.Biophys.Res.Commun.239. 488-492 (1997)
• [Publications] Fukuoka,M.: "Identification of N-WASP homologs in human and rat brain." Gene. 196. 43-48 (1997)
• [Publications] Miki,H.: "Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP." Nature. 391. 93-96 (1998)
• [Publications] Yu,H.: "Phosphorylation of PLC-γ-1 on tyrosine residue 783 by PDGF regulates re-organization of the cytoskeleton." Biochem.J.(in press).
• [Publications] Yu,H.: "Phosphatidylinositol 4,5-bisphosphate reverses the inhibition of RNA transcription caused by histon H1." Eur,J.Biochem.(in press).
• [Publications] Nakashima,S.: "Role of signal-transducing phospholipase A2,C and D in platelet activation:in"Advances in Molecular and Cell Biology Series,Vol.17,The Platelet"" Lapetina,E.G.ed.,JAI Press Inc.,Greenwich, 22 (1997)
• [Publications] Nakashima,S.: "Platelet phospholipase C and D:in "Handbook of Experimental Pharmacology,Vol.126,Platelets and their Factors"" Bruchhausen,V.and Walter,U.eds.,Spring-Verlag,Heidelberg, 16 (1997)
• [Publications] Ohguchi,K.: "Phospholipase D development during differentiation of HL60 cells:in"Molecular Biology Intelligence Unit,Phospholipase D"" Daniel,L.W.ed.,R.G.Landes Company(in press),
• [Publications] Sada,K.: "Effect of lectins on protein kinase activity:in"Lectin Methods and Protocols"" Rhodes,J.M.and J.D.Milton,J.D.eds.,Humana Press Inc., 10 (1998)
• [Publications] Fukami,K.: "Regulation of α-actinin and vinculin functions by polyphosphoinositides:in"The 1998 Molecular Biology Intelligence Unit Book,G protein,Cytoskeleton and Cancer"" Aktories,K.ed,Springers and DG Landes(in press),
• [Publications] Takenawa,T.: "Ash/Grb2 downstream signalling-signals to cytoskeleton:in"Cytoskeleton and G proteins in the Regulation of Cancer"" The proceedings of the 17th international symposium on cancer(in press),
• [Publications] Ohno,S.: "The distinct biological potential of PKC isotypes:in"Protein Kinase C"" Parker,P.J.and Dekker,L.V.eds.,R.G.Lands Company,Springer, 19 (1997)
• [Publications] Yoneda,Y.: "Molecular mechanism of nucleocytoplasmic transport of proteins through the nuclear membrane:in"Membrane Proteins"" Hamasaki,N.,and Mihara,K.eds.,S.Karger AG,Switzerland, 8 (1997)
• [Publications] Imamoto,N.: "Import and export of proteins at the nucleus:in"Protein Targeting"" Phoenix,D.ed.,Portland Press,London(in press),
• [Publications] Wakasugi,H.: "The elevated serum level of thioredoxin in patients with malignant diseases and chronic inflammatory diseases:in"Oxidative Stress in Cancer,AIDS,and Neurodegeneratibe Diseases"" Montagnier,L,Olivier,R.,and Pasquier,C.,eds.Marcel Deller,Inc., 10 (1998)
• [Publications] Kobayashi,H.: "Lysenin,a novel bioactive protein isolated from coelomic fluid of the earthworm Eisenia foetida:structure,secretion and biological activity:in"Neurobiology:Prospect and Perspectives"" Springer-Verlag,Heidelberg, 15 (1997)