Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Koichi National Research Institute of Aquaculture, 養殖研究所・遺伝育種部, 主任研究官
SAITOH Kenji Tohoku National Fisheries Research Institute, 東北区・水産研究所, 室長
SUZUKI Nobuhiro National Research Institute of Aquaculture, 養殖研究所・栄養代謝部, 室長
YASUE Hiroshi National Institute of Animal Industry, 畜産試験場・育種部, 上席研究官
田 祥鱗 祥明大学校, 教授
伍 漢霖 上海水産大学, 教授
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Budget Amount *help |
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1999: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1998: ¥5,500,000 (Direct Cost: ¥5,500,000)
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Research Abstract |
Systematics and biogeography of Chinese and Korean bitterlings were studied. In 1998, we went to Shanghai City, Heilongjian and Fujian Provinces in China, and Kyonggi Province in Korea, where we studied classification, karyotypes and collected samples for DNA experiments, with Chinese and Korean investigators. In 1999, we went to Shanghai City, Anhui and Zhejiang Provinces in China, and Kyonggi, Kangwon, Chungchongbuk, Chungchongnam, Kyongsangbuk, Kyongsangnam, Chollabuk, Chollanam Provinces in Korea. As the results of the Expedition from 1998 to 1999, following points were found : 1) Bitterlings of 2 genera, 12 species-subspecies in China and 3 genera, 13 species-subspecies in Korea were collected. 2) Rhodeus ocellatus ocellatus and Acheilognathus sp. were collected for the first time in Heilongjian Province. 3) R. sp. collected from Namhan River system in Korea is similar to R. sericeus, but is different from R. sericeus by strong simple rays of dorsal and anal fins and nuptial color
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of males, and morphology of the anteriormost part of the yolk-sac of the newly hatched larva. They had the same chromosomal number, 2n=48, but might be different in the C- and the Ag-band patterns. 4) A bitterling having 2n=46 was found for the fienst time in Fujian Province. 5) The karyotype change from 2n=48 to 2n=46 in Rhodeus might have been caused by the inversion and the tandem fusion. 6) The clear karyotype of Tanakia signifer is the first report. It had a few acrocentric chromosome paris. This may be a characteristic in Tanakia. 7) A highly repetitive DNA family with partially tandem and partially interspersed elements was isolated from two subspecies of Chinese bitterlings R. atremius suigensis and R. ocellatus ocellatus. This repetitive family may be a useful landmark for future chromosomal studies. 8) According to the RFLP analysis of mtDNA in R. ocellatus from China, Korea and Japan, it seems that Japanese R. ocellatus introduced from mainland China shows polyphyly and some lines are distributed in China. Less
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