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Moleculer Phylogenetic Study of Cichlid Fishes in Africa

Research Project

Project/Area Number 10041161
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section海外学術
Research Field 遺伝
Research InstitutionTOKYO INSTITUTE OF TECHNOLOGY

Principal Investigator

OKADA Norihiro  Tokyo Institute of Technology Professor Graduate School of Biosciense & Biotechnology, 大学院・生命理工学研究科, 教授 (60132982)

Co-Investigator(Kenkyū-buntansha) OHSHIMA Kazuhiko  Tokyo Institute of Technology Associate Professor Graduate School of Biosciense & Biotechnology, 大学院・生命理工学研究科, 講師 (60282852)
YUMA Masahide  Kyoto University Associate Professor Center for Ecological Research, 生態学研究センター, 助教授
NISHIDA Mutsumi  University of Tokyo Professor Division of Moleculer Marine Biology, Ocean Research Institute, 海洋研究所, 教授
TAKAHASHI Kazuhiko  Tokyo Institute of Technology Research Assistant Graduate School of Bioscience & Biotechnology, 大学院・生命理工学研究科, 助手 (40302955)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsLake Victoria / Lake Malawi / Lake Tanganyika / Cichlid fish / Adaotive radiation / phylogeny / Cichlidae / SINE / レトロポゾン / アフリカ / マラウィ湖 / PCR
Research Abstract

Lake Tanganyika harbors numerous endemic species of extremely diverse cichlid fish that have been classified into twelve major taxonomic groups known as tribes. Analysis of SINE insertion data has been acknowledged as a powerful tool for the elucidation of phylogenetic relationships, and we applied this method in an attempt to clarify such relationships among these cichlids. We studied insertion patterns of 38 SINEs in total, 24 of which supported the monophyly of three clades. The other 14 loci revealed extensive incongruence in terms of the patterns of SINE insertions. These incongruence most likely stems from period of adaptive radiation. One possible explanation for this phenomenon in the extensive incomplete lineage sorting of alleles for the presence or absence of a SINE during successive speciation events during this period which took place about 5-10 million years ago.
Lake Malawi is home to more than 450 species of endemic cichlids, which provide a spectacular example of adaptive radiation. We identified six loci at which a SINE sequence had apparently been specifically inserted by retroposition in the common ancestor of all the investigated species of endemic cichlids in Lake Malawi. At another locus, unique sharing of a SINE sequence was evident among all the investigated species of endemic non-Mbuna cichlids with the exception of Rhamphochromis sp. We also characterized a locus that exhibited transspecies polymorphism with respect to the presence or absence of the SINE sequence among non-Mbuna species. This result suggests that incomplete lineage sorting and/or interspecific hybridization might have occurred or be occurring among the species in this group, which might potentially cause misinterpretation of phylogenetic data, in particular when a single-locus marker, such as a sequence in the mitochondrial DNA, is used for analysis.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Takahashi,K.,M.Nishida,M.Yuma,and N.Okada: "Retroposition of the AFC family of SINESs (short interspersed repetitive elements) before and during the adaptive radiation of cichlid fishes in Lake Malawi and related inferences about phylogeny."J.Mol.Evol.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi,K.,T.Terai,M.Nishida,and N.Okada: "A novel family of short interspersed repetitive elements (SINEs) form cichlid : The patterns of insertion of SINEs at orthologous loci support the proposed monphyly of four major groups of cichlid fishes in Lake Tanganyika."Mol.Biol.Evol.. 15. 391-407 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi, K., M.Nishida, M.Yuma, and N.Okada: "Retroposition of the AFC family of SINEs(short interspersed repetitive elements)before and during the adaptive rediation of cichlid fishes in Lake Malawi and related inferences about phylogeny"J.Mol.Evol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi, K., T.Terai, M.Nishida, and N.Okada: "A novel family of short interspersed repetitive elements(SINEs)from cichlids : The patterns of insertion of SINEs at orthologous loci support the proposed monphyly of four major groups of cichlid fishes in Lake Tanganyika."Mol.Biol.Evol.. 15. 391-407 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi,K.,M.Nishida,M.Yuma,and N.Okada: "Retroposition of the AFC family of SINESs (short interspersed repetitive elements) before and during the adaptive radiation of cichlid fishes in Lake Malawi and related inferences about phylogeny."J.Mol.Evol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takahashi,K.,T.Terai,M.Nishida,and N.Okada: "A novel family of short interspersed repetitive elements (SINEs) from cichlid : The patterns of insertion of SINEs at orthologous loci support the proposed monphyly of four major groups of cichlid fishes in Lake Tanganyika."Mol.Biol.Evol.. 15. 391-407 (1998)

    • Related Report
      2000 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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