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Epidemiological analysis of dengue virus in the Philippines

Research Project

Project/Area Number 10041201
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNagasaki University

Principal Investigator

IGARASHI Akira  Institute of Tropical Medicine, Nagasaki University, Professor, 熱帯医学研究所, 教授 (40029773)

Co-Investigator(Kenkyū-buntansha) TAKAGI Masahiro  Institute of Tropical Medicine, Nagasaki University, Professor, 熱帯医学研究所, 教授 (60024684)
HASEBE Futoshi  Institute of Tropical Medicine, Nagasaki University, Research Associate, 熱帯医学研究所, 助手 (20253693)
MORITA Kouichi  Institute of Tropical Medicine, Nagasaki University, Assistant Professor, 熱帯医学研究所, 講師 (40182240)
ESHITA Yuuki  Oita Medical University, Associate Professor, 感染予防医学, 助教授 (10082223)
NAGATAKE Tsuyoshi  Institute of Tropical Medicine, Nagasaki University, Professor, 熱帯医学研究所, 教授 (30164445)
NATIVIDAD Fi  セント, ルーク・メディカルセンター・研究及び生物工学部門, 部長
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1999: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1998: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsDF / DHF / IgM-ELISA / Philipines / FCM / Cytokine / Permeable factor / Vector / ケモカイン / 伝播 / IgM・ELISA / RT・PCR / 血管内皮透過性
Research Abstract

Seroepidemiological study was performed retrospectively on DF/DHF in Manila, the Philippines using serum samples which were kept at St. Luke's Medical Center since 1995. All serotypes of dengue viruses were prevalent in Manila and outbreaks occurred in 2 year-intervals. A rapid and sensitive quantitative assay for dengue virus based on flow cytometry (FCM) was developed using flavivirus cross reactive monoclonal antibody (FITC-6B6C-1). Dengue patient's peripheral blood leukocyte (PBL) were examined by FCM using FITC-6B6C-1 and phycoerythrin(PE) labeled anti-human leukocyte surface markers (CD3, CD14, CD16, CD19) to determine the dengue virus target cells in the blood. Clinical data of the dengue patients were collected such as blood test (Ht, anti-platelet antibody, RBC, WBC and platelet count), chest X-ray and DIC score to characterize DF and DHF patients. Besides, several cytokine levels were measured in dengue patients' sera to determine the factor related to disease severity. On the other hand, ecological studies were conducted on dengue virus vectors using ovi-traps in Tondo area (as an urban model) and Los Banos area (as a rural model) from Aug. 1998 to Jul. 1999.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Basu Dev Pandey, Igarashi A.: "Severity related molecular differences among nineteen strains of dengue type 2 virus"Microbiol. Immunol.. 44(3). 179-188 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 江下 優樹: "伝播昆虫の制御:遺伝子工学を用いた病原体耐性蚊の開発(地球規模の寄生虫対策の時代)"医学のあゆみ. 191(1). 98-103 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Basu Dev Pandey, Igarashi A.: "electron microscopic examination of Aedes albopictus clone C6/36 cells infected with dengue virus 2 at elevated incubation temperature"Acta Viologica. 42. 35-39 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 五十嵐 章: "東南アジアにおける日本脳炎とデング/デング出血熱"日本獣医師会雑誌. 51. 397-405 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 五十嵐 章: "感染症シリーズ 5.デング熱/デング出血熱"医療. 52. 326-329 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 五十嵐 章: "デング熱/デング出血熱"臨床検査. 42. 529-534 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 五十嵐 章: "ウイルス性出血熱 外来診療のすべて"メディカルビュー社(高久史麿 総監修). 2 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 五十嵐 章: "アルボウイルス感染症.知っておきたい現代感染症事情2"医歯薬出版社(中山宏明、多田 功、南嶋洋一 編集). 10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Basu Dev Pandey and Igarashi A.: "Severity related molecular differences among nineteen strains of dengue type 2 virus"Microbiol. Immunol.. 44(3). 179-188 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Basu Dev Pandey and Igarashi A.: "Electron microscopic examination of Aedes albopictus clone C6/36 cells infected with dengue virus 2 at elevated incubation temperature"Acta Viologica. 42. 35-39 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mangada M.N.M., Igarashi A.: "Molecular and in vivo analysis of eight dengue type 2 viruses isolated from patients exhibiting different disease severities"Virology. 244. 458-466 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Basu Dev Pandcy et al.: "Severity Related Molecular Differences among Nineteen Strains of Dengue Type 2 Virus"Microbiology and Immunology. 44(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mangada,M.N.M.Igarashi,A.: "Molccular and in vitro analysis of eight dengue type 2 viruses isolated from patients exhibiting different discase severities." Virology. 244. 458-466 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Pandey.B.Igarashi,A.: "Electron inicroscopie examination of Aedes albopictus clone C6/36 cells infected with dengue virus 2 of elevated incubation temperature." Acta Virology. 42. 35-39 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 五十嵐 章: "デング・デング出血熱" 臨床検査. 42. 529-534 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 五十嵐 章: "感染症シリーズ 5.デング熱/デング出血熱" 医療. 52. 326-329 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 五十嵐 章: "東南アジアにおける日本脳炎とデング熱/デング出血熱" 日本獣医師会雑誌. 51. 397-405 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 五十嵐 章: "ウイルス性出血熱 外来診療のすべて" メディカルビュー社(高久史麿 総監修), 2 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 五十嵐 章: "アルボウイルス感染症.知っておきたい現代感染事情2" 医歯薬出版社(中山宏明、多田 功、南嶋洋一 編集), 10 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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