| Project/Area Number |
10044203
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Mie University |
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Principal Investigator |
CHINZEI Yasuo Mie. Univ., Fac. of Med., Professor, 医学部, 教授 (60024709)
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| Co-Investigator(Kenkyū-buntansha) |
YUDA Masao Mie. Univ., Fac. of Med., Assistant, 医学部, 助手 (90293779)
MIURA Ken Mie. Univ., Fac. of Med., Lecturer, 医学部, 講師 (60219582)
ANDO Katsuhiko Mie. Univ., Fac. of Med., Associate Professor, 医学部, 助教授 (90024710)
WALKER V. クイーンズ大学, 生物, 教授
WYATT G. クイーンズ大学, 生物, 教授
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1999: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | Juvenile Hormone / ecdysone / metamorphosis / transcription factor / GRF / ECR / USP / JH receptor / リセプター / 遺伝子発現 / 転写調節因子 / シアノプロテイン / ヴィテロジェニン |
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| Research Abstract |
Insect juvenile hormone (JH) is an important hormone for insect to regulate metamorphosis, diapause, polymorphism, reproduction and so on. Molecular mechanism of mode of action of JH have been studied, and shown to regulate the expression of genes related to those physiological phenomena. However, JH receptor has not been identified and isolated.
A PCR approach has been used to isolate, form Bombyx mori, a cDNA encoding a novel orphan receptor (GRF) that is most closely related to Bombyx bFTZ-F1 and to the vertebrate germ cell nuclear factor. The major GRF mRNA is detected in most tissues as an 8-kb transcript whose amount follows the circulating ecdysteroid concentration with a delay. The expression pattern of GRF is similar to that of the Bombyx homologue of the Drosophila early late gene DHR3, and precedes that of bFTZ-F1 in all stages and tissues examined. The GRF protein is thus likely to be required in many tissues, but in a temporally restricted manner suggesting that GRF has a well-defined function in the ecdysteroid-induced transcription cascade. The GRF protein binds in vitro to a single oestrogen receptor half-site AGGTCA preceded by a 50 -TCA extension, and is therefore a potential co-regulator of the orphan receptors bFTZ-F1 and DHR39.
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