Molecular mechanisms of mutagenesis caused by transletion DNA synthesis
| Project/Area Number |
10044208
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
MAKI Hisaji Nara Institute of Science and Technology, Grad. Sch. of Biol. Sci., Professor, バイオサイエンス研究科, 教授 (20199649)
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| Co-Investigator(Kenkyū-buntansha) |
UMEZU Keiko Nara Institute of Science and Technology, Grad. Sch. of Biol. Sci., Research Associate, バイオサイエンス研究科, 助手 (20223612)
AKIYAMA Masahiro Nara Institute of Science and Technology, Grad. Sch. of Biol. Sci., Associate Professor, バイオサイエンス研究科, 助教授 (80273837)
関 峰秋 奈良先端科学技術大学院大学, バイオサイエンス研究所, 教務職員 (40304167)
松本 吉博 Fox Chase Cancer Center, Associate
FUCHS Robert CNRS, URR9003, Director
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | DNA polymerase / Carciniogen / Mutagen / DNA replication / DNA repair / Error-pronerepair / SOS response / SOS repair |
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| Research Abstract |
The DNA replication apparatus stops when it meets DNA lesions on template. Cell recognizes such a replication fork block and starts to operate several cellular functions that overcome the replication fork block. It has been known that post-replicative recombination repair and SOS repair are induced upon DNA replication fork block in E.coli cells. Most recently, these functions appeared to be operating also in eukaryotic cells. Aim of this project was to clarify molecular mechanisms of translesion DNA synthesis in E.coli and higher eukaryote.
1.Assay for mutagenesis in in vitro DNA replication using E.coli replicative apparatus : We have established several systems for DNA replication in vitro reconstituted from purified replicative enzymes and succeeded in determining forward mutations caused by the DNA replication. It appeared that base substitution mutations were induced at several hot spots by DNA replication errors resulting from spontaneous DNA damages. Furthermore, the most frequent replication errors by the replicative apparatus were found to be those leading to single base frameshifts. This type of replication errors were highly specifically made at runs of same nucleotides in the template sequence.
2.Analyses of natural pausing sites for DNA chain elongation using in vitro DNA replication system : We investigated pausing sites for DNA chain elongation by DNA polymerase III holoenzyme of E.coli using in vitro system with M13 single-stranded phage DNA as template. 8 strong pausing regions were found, and each of them consists of several pausing sites. About half of the pausing sites are able to form stable hair-pin structures.
3.Identification of factors involved in translesion DNA synthesis in Xenopus laebis oocyte extracts : Using synthetic oligomer DNA that contains DNA lesion at a particular site, we have searched enzymaticactivities for translesion DNA synthesis. At least three different pathways were found.
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Report
(3 results)
Research Products
(9 results)
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[Publications] Shingo Fujii, Masahiro Akiyama, Kazuhiro Aoki, Yutaka Sugaya, Kumiko Higuchi, Mina Hiraoka, Youhei Miki, Naotoshi Saitoh, Kaoru Yoshiyama, Keiichi Ihara, Mineaki Seki, Eiichi Ohtsubo and Hisaji Maki: "DNA Replication Errors Produced by the Replicative Apparatus of Escherichia coli"J. Mol. Biol.. 289. 835-850 (1999)
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