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Chromosomal characterization of Japano-Chinese plants

Research Project

Project/Area Number 10044209
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionHiroshima University

Principal Investigator

KONDO Katsuhiko  Hiroshima Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (00110817)

Co-Investigator(Kenkyū-buntansha) TAKAMIYA Masayuki  Kumamoto Univ., Faculty of Science, Associate Professor, 理学部, 助教授 (70179555)
DEGUCHI Hironori  Hiroshima Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (60117017)
HOSHINO Takuji  Okayama Univ.of Science, Professor, 総合情報学部, 教授 (10122392)
OGINUMA Kazuo  Kochi Women's Univ., Faculty of Human Life and Environ.Sci., Professor, 生活科学部, 教授 (30106794)
HIZUME Masahiro  Ehime Univ., Faculty of Education, Professor, 教育学部, 教授 (30116967)
陳 之瑞  中国科学院, 植物研究所, 教授
洪 徳元  中国科学院, 植物研究所, 教授
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2000: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1999: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1998: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsConservation biology / Endangered plant species of Japano-Chinese element / Conservation lines / Molecular cytogenetics / Molecular systematics / ISH / GISH / Flora of Japan / 染色体学 / 日本 / 中国 / 野生植物 / 絶滅危惧植物種 / 染色体 / 中華人民共和国 / 保全
Research Abstract

During the course of this three-year-grant-project form 1998-2000, we have studied on conservation and chromosomal characterization by molecular cytogenetics and molecular systematics in certain plant species common to Japan and China. We have visited and studied plant habitats in Jilin, Heilongjian, Nei Mongol Zizhiqu, Shaanxi, Gansu, Xinjiang Uygur Zizhiqu and Sichuan Provinces, China and have investigated and collected certain species and genera in the families of bryophytes, pteridophytes, Asteraceae (Compositae), Cyperaceae, Liliaceae, Orchidaceae, Ranunclaceae, Butulaceae, Hamamelidaceae, Pinaceae, Saxifragaceae, Rosaceae, Theaceae, Labiatae, Scrphulariaceae and so on for own researches. Those species were firstly counted their chromosome numbers and were, then, determined their karyotypes. Furthermore, we characterized those plants by in situ hybridization (ISH) including fluorescent in situ hybridization (FISH), genomic in situ hybridization (GISH), DNA sequencing, and so on. W … More e have successfully, very firstly marked by ISH using 5S rDNA certain super small chromosomes in woody species in three genera of the Betulaceae. This methodology may promise to analyze populations of woody plants which have commonly super small chromosomes. Multi-color FISH improvement in some members of the Pinaceae has been rapidly progressed. The most significant research progress has been made in Chrysanthemum sensu lato. Intergeneric hybridizations in various cross combinations were artificially made and immature embryos were harvested and rescued in tissue culture condition to obtain hybrid plants which were used for GISH.Thus, GISH in chrysanthemum hybrid chromosomes made it possible to discover new phenomena on somatic reciprocal translocations of chromosome segments, visualization and isolation of chromosomes in resting nuclei and arien chromosomes. These technologies may be useful for further investigations of chromosomes. Mosla, the Labiatae has been detected in molecular systematics and showed very interesting interspecific relationships among the Japano-Chinese species. Since all of the plant species collected during the project operation have been established cultivation lines and conserved for laboratory base, they would be investigated deeply, genetically and clarified and justified their systems in flora of Japan and China, and then, they would become important biological research materials. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (58 results)

All Other

All Publications (58 results)

  • [Publications] Tsuneo Funamoto: "A chromosome study of tree Parnassia species collected in the Qin Ling Mountains, Shaanxi Province, China"Chromosome Science. 2. 111-115 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Hizume: "Fluorescence chromosome banding in a Chinese larch, Larix chinensis Beissn"Chromosome Science. 2. 95-98 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuneo Funamoto: "A chromosome study of five Saxifraga species collected in the Qin Ling Mountains, Shaanxi Province, China"Chromosome Science. 2. 145-150 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuhiko Kondo: "Fluorescence genomic in situ hybridization identifies recipocal translocation of somatic chromosomes and origin of extra chromosome by an artificial, intergeneric hybrid between"Chromosome Science. 3. 15-19 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiromori Deguchi: "Genetic differentiation within sibling species of Conocephalum conicum (Marchantiales : Hepaticae) in East Asia"Bryological Research. 7・8. 249-256 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuhiko Kondo: "Isolation of a particular chromosome of Ajiania remotipinna in a chromosome complement of an artificial F1 hybrid of Dendranthema lavandulifolia x Ajania remotipinna by use of"Chromosome Science. 3・1. 21-28 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masahiro Hizume: "Identification of chromosomes in two Chinese spruce species by multicolor fluorescence in situ hybridization"Chromosome Science. 3・1. 37-41 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuhiko Kondo: "Chromosome studies in chrysanthemum flora of Taiwan. 1. Dendranthema horaimontana(Masam.) S.S.Ying and D.morii (Hayata) Kitam."Chromosome Srience. 3・1. 49-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuhiko Kondo: "Karyotype stability of Smilacina japonica"Chromosome Science. 3・2. 79-82 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuo Oginuma: "Karyotype stability of Smilacina japonica"Chromosome Science. 3・2. 83-86 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuneo Funamoto: "A cytotaxonomy of six species of Chrysosplenium collected in the Qin Ling Mountains in Shaanxi Province, China"Chromosome Science. 3・2. 87-91 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisakazu Ogura: "A karyological study of Allium grayi Regel and A.chinense G.Don in Sichuan Province, China"Chromosome Science. 3・3. 119-122 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisakazu Ogura: "Polyploidy distribution and karyotype variation in several wild population of Allium grayi Regel (Liliaceae) in Okayama Prefecture, Japan"Cytologia. 65. 419-427 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuo Oginuma: "Chromosomal evolution of three genera in the Betulaceae concerning polyploidy revealed by in situ hybridization using 5S rDNA"Chromosome Science. 4・2(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuneo Funamoto: "A comparative chromosomal study of five species of Chrysosplenium collected in the northern part of Sichuan Province, China"Chromosome Science. 4・2(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shi-ling Zhou: "Genetic diversity and phylogeny of Mosla japonica (Labiatae) complex revealed by randomly amplified polymorphic DNA(RAPD) markers"Acta Phytotaxonomica Sinica. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuneo Funamoto: "A cytological observation of Rodgersia aesculifolia Batalin (Saxifragaceae) collected in Shaanxi and Sichuan Provinces, China"Chromosome Science. 4(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuhiko Kondo: "Molecular cytogenetic identification of the parental genomes in the intergeneric hybrid between Leucanthemella linearis and"Caryologia.. 54(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Funamoto, T.: "A chromosome study of three Parnassia species collected in the Qin Ling Mountains, Shaanxi Province, China"Chromosome Science. 2. 111-115 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hizume, M.: "Fluorescence chromosome banding in a Chinese larch, Larix chinensis Beissn"Chromosome Science. 2. 95-98 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Funamoto, T.: "A chromosome study of five Saxifraga species collected in the Qin Ling Mountains, Shaanxi Province, China"Chromosome Science. 2. 145-150 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo, K.: "Fluorescence genomic in situ hybridization identifies reciprocal translocation of somatic chromosomes and origin of extra chromosome by an artificial, intergeneric hybrid between Dendranthema japonica and Tanacetum vulgare"Chromosome Science. 3. 15-19 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Deguchi, H.: "Genetic differentiation within sibling species of Conocephalum conicum (Marchantiales : Hepaticae) in East Asia"Bryological Research. 7. 249-256 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo, K.: "Isolation of a particular chromosome of Ajania remotipinna in a chromosome complement of an artificial F_1 hybrid of Dendranthema lavandulifolia x Ajania remotipinna by use of genomic in situ hybridization"Chromosome Science. 3. 21-28 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hizume, M.: "Identification of chromosomes in two Chinese spruce species by multicolor fluorescence in situ hybridization"Chromosome Science. 3. 37-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo, K.: "Chromosome studies in chrysanthemum flora of Taiwan. 1. Dendranthema horaimontana (Masam.) S.S.Ying and D.morii (Hayata) Kitam."Chromosome Science. 3. 49-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo, K.: "Karyotype stability of Smilacina japonica"Chromosome Science. 3. 79-82 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Oginuma, K.: "Karyomorphology of three species of Spiraea (Rosaceae) in China"Chromosome Science. 3. 83-86 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Funamoto, T.: "H.A cytotaxonomy of six species of Chrysosplenium collected in the Qin Ling Mountains in Shaanxi Province, China"Chromosome Science. 3. 87-91 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogura.H.Z.and Hong, D.: "A karyological study of Allium grayi Regel and A.chinense G.Don in Sichuan Province, China"Chromosome Science. 3. 119-122 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogura, H.Miyamoto, T.: "Polyploidy distribution and karyotype variation in several wild populations of Allium grayi Regel (Liliaceae) in Okayama Prefecture, Japan"Cytologia. 65. 419-427 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Oginuma, K.Kondo, K.: "Chromosomal evolution of three genera in the Betulaceae concerning polyploidy revealed by in situ hybridization using 5S rDNA"Chromosome Science. 4 (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Funamoto, T.: "H.A comparative chromosomal study of five species of Chrysosplenium collected in the northern part of Sichuan Province, China"Chromosome Science. 4 (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhou, S.: "Genetic diversity and phylogeny of Mosla japonica (Labiatae) complex revealed by randomly amplfied polymorphic DNA (RAPD) markers"Acta Phytotaxonomica Sinica. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Funamoto, T.H.and Ogura, H.: "A cytological observation of Rodgersia aesculifolia Batalin (Saxifragaceae) collected in Shaanxi and Sichuan Provinces, China"Chromosome Science. 4 (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo, K.: "Molecular cytogenetic identification of the parental genomes in the intergeneric hybrid between Leucanthemella linearis and Nipponanthemum nipponicum during meiosis and mitosis"Caryologia. 54 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisakazu Ogura: "Polyploidy distribution and karyotype variation in several wild population of Allium grayi Regel (Liliaceae) in Okayama"Cytologia. 65. 419-427 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kazuo Oginuma: "Chromosomal evolution of three genera in the Betulaceae concerning polyploidy revealed by in situ hybridization using 5S rDNA"Chromosome Science. 4・2(in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsuneo Funamoto: "A comparative chromosomal study of five species of Chrysosplenium collected in the northern part of Sichuan Province, China"Chromosome Science. 4・2(in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shi-liang Zhou: "Genetic diversity and phylogeny of Mosla iaponica (Labiatae) complex revealed by randomly amplified polymorphic DNA (RAPD) markers"Acta Phytotaxonomica Sinica. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsuneo Funamoto: "A cytological observation of Rodgersia aesculifolia Batalin (Saxifragaceae) collected in Shaanxi and Sichuan Provinces, China"Chromosome Science. 4・2(in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katsuhik Kondo: "Molecular cytogenetic identification of the parental genomes in the intergeneric hybrid between Leucanthemella linearis and"Caryologia. 54(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hisakazu Ogutra: "A karyological study of Allium grayi and A・chinense G・Don in Sichuan Province, China"Chromosome Science. 3・3. 119-122 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masahiro Hizume: "Molecular cytogenetic studies on sex chromosomes and proximal heterochromatin containing telomere-like sequence in Cycas revoluta"Chromosome Science. 2・2. 63-72 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masahiro Hizume: "Fluorescence chromosome banding in a Chinese larch,Larex chineie Beissn"Chromosome Science. 2・2. 95-98 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuneo Funamoto: "A karyomorphological comparison of four Saxifraga species collected in the western part of Sichuan Province,China"Chromosome Science. 2・2. 103-109 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuneo Funamoto: "A chromosome study of three Parnassia species collected in the Qin Ling Mountains,Shaanxi Province,China"Chromosome Science. 2・2. 111-115 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuneo Funamoto: "A chromosome study of five Parnassia species collected in the Qin Ling Mountains,Shaanxi Province,China"Chromosome Science. 2・3. 145-150 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuhiko Kondo: "Fluorescence genomic in situ hybrikization identifies reciprocal translocation and origin of extra chromosome by an artificial,intergeneric hybrid between Dendranthema japonica and Tanacetum vulgare"Chromosome Science. 3・1. 15-19 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Magdy Hussein Abd El-Twab: "Isolation of a particular chromosome of Ajania remotipinna in a chromosome complement of an artificial F_1 hybrid of Dendranthema lavandulifolia X Ajania remotipinna by use of genomic in situ hybridization"Chromosome Science. 3・1. 21-28 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masahiro Hizume: "Identification of chromosomes in two Chinese spruce spesies by multicolor fluorescence in situ hybridization"Chromosome Science. 3・1. 37-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuhiko Kondo: "Chromosome studies in chrysanthemum flora of Taiwan 1.Dendranthema horaimontana (Masam.)S.S.Ying and D.morii (Hayata) Kitam."Chromosome Science. 3・1. 49-54 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Misao Itouga: "Genetic differntiation within sibling speciesof Conocephalum conicum (marchantiales:Hepaticae)in East Asiz"蘚苔類研究(Bryol.Res.). 7・8. 249-256 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nirikazu Tagashira: "Karyotype stability of Smilacina japonica"Chromosome Science. 3・2. 79-82 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kazuo Oginuma: "Karyomorphology of three species of Sporaea (Rosaceae)in China"Chromosome Science. 3・2. 83-86 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuneo Funamoto: "A chtotaxonomy of six species of Chrysosplenium collected in the Qin Ling Mountains in Shaanxi Province,China"Chromosome Science. 3・2. 87-91 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masahiro Hizume: "Fluorescence chromosome banding in a Chinese larch,Larix chinensis Beissn." Chromosome Science. 2・2(in press). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuneo Funamoto: "A Chromosome study of Parnassia species collected in the Qin Ling Mountains,Shaanxi Province,China" Chromosome Science. 2・2(in press). (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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