| Project/Area Number |
10044213
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
遺伝
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
KOMANO Teruya Tokyo Metropolitan University, Dept Biology, Professor, 理学研究科, 教授 (00087131)
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| Co-Investigator(Kenkyū-buntansha) |
FURUYA Nobuhisa Tokyo Metropolitan University, Dept Biology, Assistant, 理科研究科, 助手 (50244413)
井上 すみ子 ジョンソン医科大学, 生化学部, 準教授
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Myxobacteria / Development / Spore formation / fruA gene / fruB gene / Protein kinases |
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| Research Abstract |
Myxobacteria are unique gram-negative bacteria which undergo multicellular development. When nutrients are depleted in a solid medium, cells aggregate to form mounds which eventually convert to fruiting bodies. Rod-shaped cells differentiate to round or ovoid spores. During the development of Myxococcus xanthus, five cycles of intercellular signal exchanges are known to occur. The fruA gene encodes a putative 25-kDa transcription factor essential for the development of M.xanthus. In the present study, we have demonstrated the presence of a putative repressor X for fruA expression by gel shift experiments. Binding competition experiments suggested the X recognition sequence. Structure of protein P15, expression of which was inhibited by the fruA mutation, was analyzed by mass spectroscopy. Insertion of kanamycin-resistance DNA fragment into ORF134 resulted in failure of development, suggesting the presence of ORF134-fruB operon responsible for M.xanthus development. Two promoter structures were found for this operon : One promoter works during vegetative growth, while both two promoters work during development. M.xanthus lonD was found to be a heat shock gene. Expression of lonD was independent on sigBCE genes encoding an Escherichia coli RpoH homolog, but dependent on hsfA and sigA encoding a RpoD homolog. The hsfA gene encodes a putative effecter of a two-component system, which was phosphorylated by HsfB kinase. Phosphorylated-HsfA bound to promoter sequence for lonD gene. M.xanthus carries at least 13 eukaryotic-like protein Ser/Thr pkn kinases. Many gene products were found to interact with Pkn kinases by two-hybrid experiments.
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