| Project/Area Number |
10044225
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University (1999)
Hokkaido University (1998) |
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Principal Investigator |
IKUTA Kazuyoshi Research Institute for Microbial Diseases, Osaka University Professor, 微生物病研究所, 教授 (60127181)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Etsuo Institute of Immunological Science, Hokkaido University Associate Professor, 免疫科学研究所, 助教授 (00160903)
IWAHASHI Kazuhiko Azabu University, Dept. of Environmental Health Associate Professor, 環境保健学部, 助教授 (00232695)
LUFTIG Ronal LSU Medical Center, Professor
RICHIT Juerg Univ. of Giessen, Inst. of Virology, Associate
鐘 照華 ハルビン医科大学, 微生物, 副教授
ARUNAGIRI Ch Royal Childrens Hospital Schizophrenia R, Head
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | HIV-1 / Borna Disease Virus / Subtype / Subtype B / Subtype E / Molecular clone / Nef / Psychiatric disease / HIV / BDV / AIDS脳症 / 遺伝子変異 / 単クローン抗体 / 潜伏感染 / トランスジェニックマウス |
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| Research Abstract |
1) HIV-1 easily induce mutations and therefore HIV-1 can be classified into several subtypes. However, most of the HIV-1 research has been worked on subtype B which distribute in Western countries and Japan. To understand AIDS pathogenesis, comparative studies among different subtypes of HIV-1 are effective.
We have obtained two isolates (named 95TNIH022 and 95TNIH047) of HIV-1 subtype E by the culture of peripheral blood mononuclear cells (PBMCs) from infected Thai carriers. In addition, we could prepare their full-length DNA by using DNA fragment amplified by PCR using total DNAs from the infected PBMCs. Full-length sequences of the two subtype E showed mosaic HIV-1 of subtypes A/E, as reported previously. These DNAs in plasmid (p95TNIH002 and p95tnih047) were found to be infectious. Further, we showed that there were low or no antibody responses to Nef in subtype E-infected individuals, whereas abut 30-40% of the subtype B-infected individuals induced antibodies to Nef, although these antibodies were reactive only against subtype B, but not subtype E, Nef protein.
2) In Costa Rica, we found that several cases in horses and patients with schizophrenia were positive for BDV infection. In addition, we found that BDV was positive in Chinese patients with schizophrenia.
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